Olympus BX51 Instructions Manual

This instruction manual is for the Olympus System Microscopes Models BX51 and BX52. To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of this micro- scope, we recommend that you study this manual thoroughly before operating the microscope. Retain this instruction manual in an easily accessible place near the work desk for future reference.

3-2 Focusing Block ...................................................................................................................................................................................... 11 1 Voltage Indication; 2 Using the Light Intensity Preset Switch; 3 Using the Filters 21-23 24-25 26 27-29 Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to assemble the microscope yourself, please read section 7, “ASSEMBLY” (pages 27 to 29) carefully. IMPORTANT — Be sure to read this section for safe use of the equipment. — 1 NOMENCLATURE 2 3 USING THE CONTROLS 3-1 Base................................................................................................................................................................................................................. 8-10 3-3 Stage............................................................................................................................................................................................................ 12-14 3-4 Observation Tube...................................................................................................................................................................... 15-17 3-5 Condenser.......................................................................................................................................................................................... 18-19 1 Centering the Condenser; 2 Compatibility of Objectives and Condensers 3-6 Immersion Objectives................................................................................................................................................................. 20 1 Using Immersion Objectives 3-7 Objectives with Correction Collar ............................................................................................................................. 20 4 TROUBLESHOOTING GUIDE 5 SPECIFICATIONS 6 OPTICAL CHARACTERISTICS 7 ASSEMBLY PROPER SELECTION OF THE POWER SUPPLY CORD ................................................................. 30-31 1 Replacing the Fine Adjustment Knob; 2 Adjusting the Coarse Adjustment Knob Tension; 3 Pre-focusing Lever 1 Placing the Specimen; 2 Adjusting the X- and Y-Axis Knob Tension; 3 Rotating the Stage; 4 Adjusting the Stage Height 1 Adjusting the Interpupillar Distance; 2 Adjusting the Diopter; 3 Using the Eye Shades; 4 Using Eyepiece Micrometer Disks; 5 Selecting the Light Path; 6 Adjusting the Tilt 1-3 — See this section for the replacement of the light bulb. —

12V50WHAL-L (LIFE JC) This microscope employs a UIS (Universal Infinity System) optical design, and should be used only with UIS eyepieces, objectives and condensers for the BX2 series. (Some of the modules designed for the BX series are also usable. For details, please consult Olympus or the catalogues.) Less than optimum performance may result if inappropriate accessories are used. 1. Install the microscope on a sturdy, level table or bench so as not to block the air vents on the underside of the base. Do not place the microscope on a flexible surface, as this could result in blocking the air vents and cause overheating or a fire. 2. The surfaces of the lamp housing on the rear of the microscope will become extremely hot during operation. When installing the microscope, make sure to allow ample free space (10 cm or more) around and in particular above the lamp housing. 3. When installing the microscope, route the power cord away from the lamp housing. Should the power cord come in contact with the hot lamp housing, the power cord could melt and cause electric shock. 4. To avoid potential shock hazards and burns when replacing the light bulb, set the main switch @ to “ ” (OFF) then disconnect the power cord from the wall outlet in advance. Whenever you replace the bulb during use or right after use, allow the lamp socket ‚ and bulb to cool before touching. (Fig. 1) #The microscope also incorporate a fuse (this should be replaced by the manufacturer or an authorized agent). 5. Always use the power cord provided by Olympus. If no power cord is provided, please select the proper power cord by referring to the section “PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of this instruction manual. If the proper power cord is not used, product safety performance cannot be warranted. 6. Always ensure that the grounding terminal of the microscope and that of the wall outlet are properly connected. If the equipment is not grounded, Olympus can no longer warrant the electrical safety performance of the equipment. 7. Never insert metallic objects into the air vents of the microscope frame as this could result in electrical shock, personal injury and equipment damage. @

Before use, carefully read the instruction manual. Improper use could result in personal injury to the user and/or damage to the equipment. Indicates that the main switch is ON. Indicates that the main switch is OFF. Warning engraving position Lamp housing (Warning against high temperature) Fig. 2 1 Getting Ready Safety Symbols The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment in the safest possible manner. Warnings Warning engraving is placed at parts where special precaution is required when handling and using the microscope. Always heed the warnings. 1. A microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact. 2. Do not use the microscope where it is subjected to direct sunlight, high temperature and humidity, dust or vibrations. (For the operating condi- tions, refer to section 5, “SPECIFICATIONS”.) 3. When moving the microscope, carefully carry it with the grasping part on the rear of the arm and the base as shown in Fig. 2 (Weight: approx. 16 kg). #If a module such as a photographic device is installed, the micro- scope system will be very heavy. Remove the intermediate attach- ment before carrying. #Damage to the microscope will occur if you grasp it by the stage, coarse/fine adjustment knob or binocular section of the observa- tion tube. 4. The BX51/52 series can be used with up to two intermediate attach- ments (e.g. a U-CA magnification changer, U-EPA2 eyepoint adjuster, etc.). For restrictions when using two intermediate attachments, make sure to read the instruction manual provided with the respective intermediate attachments.

1. Clean all glass components by wiping gently with gauze. To remove fingerprints or oil smudges, wipe with gauze slightly moistened with a mixture of ether (70%) and alcohol (30%). Since solvents such as ether and alcohol are highly flammable, they must be handled carefully. Be sure to keep these chemicals away from open flames or potential sources of electrical sparks –– for example, electrical equip- ment that is being switched on or off. Also remember to always use these chemicals only in a well-ventilated room. 2. Do not attempt to use organic solvents to clean the microscope components other than the glass components. To clean them, use a lint-free, soft cloth slightly moistened with a diluted neutral detergent. 3. Do not disassemble any part of the microscope as this could result in malfunction or reduced performance. 4. When not using the microscope, keep it covered with a supplied dust cover. 3 Caution If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the equipment may also be damaged. Always use the equipment as outlined in this instruction manual. The following symbols are used to set off text in this instruction manual. : Indicates that failure to follow the instructions in the warning could result in bodily harm to the user and/or damage to equipment (including objects in the vicinity of the equipment). # : Indicates that failure to follow the instructions could result in damage to equipment. } : Indicates commentary (for ease of operation and maintenance).

Diopter adjustment ring (Page 15) Slide holder (Page 12) Aperture iris diaphragm ring (Page 19) X-axis knob (Page 13) Y-axis knob (Page 13) Pre-focusing Lever (Page 11) Filter mount (Page 9) Field iris diaphragm ring (Page 18) Allen screwdriver Condenser height adjustment knob (Page 18) Voltage indicator LED Main switch (Page 1) Light intensity preset switch (Page 8) Light intensity preset adjustment screw (Page 8) Fine adjustment knob (Page 11) Coarse adjustment knob (Page 11) Coarse adjustment tension adjustment ring (Page 11) Brightness adjustment knob (Page 8) (Lamp voltage adjustment knob) (accommodation position) (Detachable) } If you have not yet assembled the microscope, read section 7, “ASSEMBLY” (pages 27 to 29). Transmitted light specification models BX51TF or BX52TF microscope frame. Filter knobs (Page 9) Optional filter knob (Page 9) Light path selector knob (Page 16) ND6, ND25 and LBD from the front to the rear

BX51TRF and BX52TRF microscope frames NOTE * For the reflected illuminators and reflected lamp housings, please also refer to their instruction manuals. The controls are identical to those of the transmitted light specification models except for the transmitted/ reflected light switch and the reflected light system (vertical illuminator, reflected lamp housing). Reflected light illuminator* · Universal illuminator (BX-URA2) · Fluorescence illuminator (BX-RFA) Reflected lamp housing* · 100 W mercury burner housing (U-LH100HG) · 100 W mercury apo-burner housing (U-LH100HGAPO) · 75 W xenon lamp housing (U-LH75XEAPO) · 100 W halogen lamp housing (U-LH100-3) · System lamp housing (U-ULH, halogen bulb specification) (for exclusive use with the halogen light source)

Set the main switch to “ I ” (ON) and adjust the brightness. @Main switch ²Brightness adjustment knob Set the transmitted light (BX51TRF/BX52TRF only) ³Transmitted/reflected light switch Engage only the LBD filter. |LBD filter knob Select the light path (trinocular tube). ƒLight path selector knob Place the specimen on the stage. …Slide holder †X-/Y-axis knobs Engage the 10X objective in the light path. ‡Revolving nosepiece Bring the specimen in focus. ŠCoarse/fine adjustment knobs Adjust the interpupillary distance. Adjust the diopter. Adjust the light axis. ‰Binocular tube ‹Diopter adjustment ring ŒCondenser height adjustment knob ™Condenser centering knob Adjust the aperture iris and field iris diaphragms. šAperture iris diaphragm ring ›Field iris diaphragm ring Engage the objective to be used in the light path and bring the specimen in focus. ‡Revolving nosepiece Adjust the brightness. ²Brightness adjustment knob Engage the required filters. œFilters (P. 5) (P. 9) (P. 8)

œ › Œ Š

3-1 Base Fig. 3 @ Fig. 4 @ ² ² 1 Voltage Indication (Fig. 3) 1. Turn the brightness adjustment knob @ clockwise to increase the volt- age and make illumination brighter. 2. The numerals to the right of the lamp voltage indicator LEDs ² indicate the voltage. 2 Using the Light Intensity Preset Switch (Fig. 4) }The light intensity preset switch @ makes it possible to limit the light intensity to a preselected level regardless of the position of the bright- ness adjustment knob. The light intensity preset has been preset to optimum brightness for photography when the built-in LBD filter is engaged (approx. 9 V with marking). 1. Press the light intensity preset switch @ to the ON position. (The face of the switch lights when it is ON.) 2. Using a small flat-blade screwdriver, turn the preset adjustment screw ² to obtain the required light intensity. Turning the screw clockwise increases brightness. 3. When the light intensity preset switch is set to OFF, the brightness returns to the level set by the brightness adjustment knob. #While the light intensity preset switch is ON, turning the light bright- ness adjustment knob does not affect brightness. 3 Using the Filters (Figs. 5 - 10) }You can place a filter in the light path with one of the following methods. · Push in the knob of the desired built-in filter to engage it in the light path. (Page 9) · Place a filter on the filter mount on the base and engage in the light path. (Page 9) · Insert a filter in the U-FC filter cassette, attach it on the filter mount and engage it in the light path by sliding the filter lever. (Page 9)

Fig. 5 @ ² ³ | Each of filter knobs @ to | can be engaged into the light path by press- ing the knob in. Pressing the button again disengages the filter from the light path. Filter Type/Purpose @ ND6 (Neutral Density filter for light adjustment, transmittance 6%) ND25 (Neutral Density filter for light adjustment, transmittance 25%) LBD (for color balancing, daylight filter) OP (optional) filter set* * The set can be ordered at your local Olympus representative. ² ³ | Mounting a Single Filter (Fig. 6) A filter with a diameter of 45 mm ƒ can be placed on the filter mount on the base. If you need to place multiple filters, please purchase the U-FC filter cassette. # Even when a filter cassette is used, a filter with thickness of no more than 3 mm can still be placed on the filter mount. Using the Filter Cassette (Figs. 7 - 10) Loading Filters into Filter Cassette }The filter cassette accommodates filters with a diameter of 45 mm and thickness of 2.7 mm or less. }The filter cassette has two filter levels on the right side and one on the left side. 1. Move all filter levers to the OUT position except for the one belonging to the slot into which the filter is to be inserted. 2. Slide the lever … to the IN position. Make sure that it clicks securely into place. (Fig. 7) 3. Holding the lever in the position shown in Fig. 7, put the filter into the cassette by inserting it in the direction indicated by the arrow. 4. Insert the other two filters in the same manner.

45G-530, 45G-533, 45IF550 Green 45Y-48 Yellow 45O-560 Orange Fig. 8 Fig. 9 Fig. 10 Usable Filters Applications B&W contrast filter ‡ † Š ‰ ‹ Mounting the Filter Cassette 1. Fully loosen the filter cassette clamping screw †. (Fig. 8) 2. Align the key ‡ on the bottom surface of the filter cassette with the positioning slot Š on the filter mount, then snap the filter cassette into place from above. 3. Rotate the filter cassette to align its sides with the base. (Fig. 9) 4. Align the clamping screw † with the positioning hole ‰ on the filter mount, then tighten the screw to fasten the filter cassette. #When the filter cassette is installed, the stage may hit it when low- ered. Therefore, exercise caution when lowering the stage with the filter cassette installed. Using the Filter Cassette (Fig. 10) Up to three of the above filters can be loaded in the filter cassette. Mov- ing the levers ‹ on the left and right sides of the cassette to the IN position brings the corresponding filter into the light path.

Fig. 11 2 (Fig. 12) Fig. 12 @ @ @ ² ³ 1 Replacing the Fine Adjustment Knob (Fig. 11) Fig. 13 }The fine adjustment knob is designed detachable to prevent interference with hand during manipulation of the fine adjustment knob or X-/Y-axis knobs. Usually attach the knob on the opposite side to the X- and Y-axis knobs. 1. Using the Allen screwdriver, loosen the clamping screw @ and remove the fine adjustment knob ². 2. Remove the seal from the fine adjustment knob screw hole on the other side and attach the knob by reversing the removal procedure. 3. Attach a provided seal on the screw hole of the removed fine adjustment knob ³. }The fine adjustment knob can be operated with your fingertip or finger surface at the same time as manipulating the X- or Y-axis knob. Adjusting the Coarse Adjustment Knob Tension #Adjust the coarse adjustment knob tension using the tension ad- justment ring @. The coarse adjustment knob tension is preadjusted for easy use. How- ever, if desired, you can change the tension using the tension adjust- ment ring @. Turning the ring in the direction of the arrow increases tension, and vice versa. The tension is too low if the stage drops by itself of focus is quickly lost after adjustment with the fine adjustment knob. In this case, turn the ring in the direction of the arrow to increase tension. 3 Pre-focusing Lever (Fig. 13) }The pre-focusing lever ensures that the objective does not come in con- tact with the specimen and simplifies focusing. After focusing on the specimen with the coarse adjustment knob, turn this lever @ in the direction of the arrow and lock; the upper limit on coarse adjustment movement is set at the locked position. After changing a specimen, refocusing is easily accomplished by rotat- ing the coarse adjustment knob to reach the pre-focused position, then making fine adjustments with the fine adjustment knob. }Stage movement with the fine adjustment knob is not locked. #If you want to lower the stage to the lower limit, release the lock in advance.

#The dimensions of the slide glass should be 26 x 76 mm with thick- ness of 0.9 to 1.2 mm, and the cover glass should have thickness of 0.17 mm. #When observing very large specimens, remove the slide holder and place the specimen directly on the stage. Microscopy with Double-Slide Holder (Fig. 14) 1. Turn the coarse adjustment knob @ to lower the stage. 2. Open the spring-loaded curved finger ² on the slide holder and place one or two specimen slides on the stage from the front. 3. After placing the sides as far as they will go, gently release the curved finger. Microscopy with Single-Slide Holder (Fig. 15) The specimen side can easily be placed by sliding it into the slide holder from the front. Using an Oil Immersion Objective Adsorption of immersion oil can cause the specimen to drift. In such cases, it is recommended to use the optional BH2-SCB-3 specimen clip ³ for oil immersion objectives. (Fig. 16) Using an Oil Immersion Condenser It may be effective to use the optional U-SVRO (right-hand knob) or U- SVLO (left-hand knob) slotted stage which prevents close contact be- tween the specimen and stage.

(Fig. 17) Fig. 17 @ ² ³ | 1. Hold the X-axis knob @ and slide up the Y-axis knob ² up to expose the adjustment knobs. 2. Turning the X-axis adjustment knob ³ or Y-axis adjustment knob | clock- wise (in the direction of the arrow) increases the tension and counter- clockwise decreases it. #If the tension is adjusted to tight, a creaking sound may be heard during stage travel, and the stage stopping accuracy may be imper- iled. After long hours of use, the stage guide may be devi- ated and the stage travel range may be decreased. However, this is not malfunction and can be corrected easily as described below. [Treatment] Horizontal direction: Hold the specimen holder and move the stage guide to the left and right so that it hits the stoppers. Vertical direction: Hold the upper stage and move it to the front and rear so that it hits the stoppers. Stage Feed Knob Rubber Caps (Optional) }When the X- and Y-axis knobs are fitted with the rubber caps, the knobs can be adjusted without slipping and fine adjustment is possible by holding the knobs with a very light force. The knob rubber caps also reduce fatigue after long hours of operation. The U-SHGT thick type (thickness 5 mm) and U-SHG thin type (thickness 2 mm) knob rubbers are available. To attach the knob rubbers: First fit the larger knob rubber to the Y-axis (upper) knob from below it, then fit the smaller knob rubber to the X-axis (lower) knob from below it.

Clockwise Counterclockwise Right hand knobs 230° 20° Left hand knobs 20° 230° Fig. 19 Fig. 20 @ @ ² ³ 3 Rotating the Stage (Fig. 18) 1. Slightly loosen the stage clamping screw @. 2. The stage can be rotated both clockwise and counterclockwise by the stage clamping screw. #A click may be heard and felt during rotation. However, this is due to the construction of the substage and does not indicate a malfunc- tion. }The angle of rotation varies depending on the positions of the X- and Y- axis knobs. 4 Adjusting the Stage Height (Figs. 19 & 20) }By lowering the position of the substage, the microscope will accommo- date specimens with maximum height of 35 mm. This is useful when observing metallurgical specimens and other thick objects. 1 Lower the stage to the lower limit, then remove the stage from the micro- scope. 2 Using the Allen screwdriver, loosen the substage bracket clamping screw @ and remove the substage. 3. Turn the coarse adjustment knob and raise the focusing block ³ to where the stopper screw ² on the arm becomes visible. 4. Using the Allen screwdriver, loosen and remove the upper stopper screw ². 5. Reattach substage bracket and stage.

Fig. 21 Fig. 22 Fig. 23 Fig. 24 @ ² 1 Adjusting the Interpupillar Distance (Fig. 21) While looking through the eyepieces, adjust for binocular vision until the left and right fields of view coincide completely. The index dot · indicates the interpupillary distance. }Note your interpupillary distance so that it can be quickly duplicated. 2 Adjusting the Diopter (Figs 22 & 23) 1. Looking through the eyepiece without the diopter adjustment ring, rotate the coarse and fine adjustment knobs to bring the specimen into focus. 2. Looking through the eyepiece with the diopter adjustment ring, turn only the diopter adjustment ring @ to focus on the specimen. (Fig. 22) Using a Finder Eyepiece 1. Looking through the right eyepiece with your right eye, turn the top of the eyepiece ² until a clearly defined double crossline can be seen in the field of view. (Fig. 22 & 23) 2. Looking through the right eyepiece, rotate the coarse and fine adjust- ment knobs to bring the specimen and double crossline into simulta- neous focus. 3. Looking through the left eyepiece with your left eye, turn the diopter ad- justment ring @ to focus on the specimen. 3 Using the Eye Shades (Fig. 24) When Wearing Eyeglasses Use with the eye shades in the normal, folded-down position. This will prevent the eyeglasses from being scratched. When Not Wearing Eyeglasses Extend the folded eye shades in the direction of the arrow to prevent extraneous light from entering between the eyepieces and eyeglasses.

lar eyepieces Observation of dark specimens Middle position 20% for binocular eyepieces, 80% for TV/photogra- phy Observation of bright specimens, photography, TV observation Pulled out 100% for TV/ photography Photography, TV observation @ @ ² 4 Using Eyepiece Micrometer Disks (Fig. 25) Eyepiece micrometer disks can be inserted into WH10X-H (or WH10X) eyepieces. Use 24 mm diam. x 1.5 mm thick micrometer disks. Following Fig. 25, unscrew the micrometer mounting frame ² from the eyepiece and place a micrometer disk @ into the mounting frame. The engraving on the micrometer disk should face downward into the mi- crometer mounting frame. Screw the micrometer mounting frame back into the eyepiece sleeve. Selecting the Light Path (with the U-TR30-2 or U-SWTR-2) Slide the light path selector knob @ to select the desired light path.

(Fig. 27) }Adjust the height and tilt of the observation tube to obtain the most com- fortable viewing position. Holding the binocular section with both hands, raise or lower it to the desired position. #Never attempt to force the binocular section past the upper or lower stop position. Applying excessive force could destroy the limiting mechanism. }The U-TBI3 can be used in combination with only one intermediate at- tachment. }If you need photography using the U-TBI3, use the U-TRU trinocular tube. With the U-ETBI (Fig. 28) The U-ETBI is an ergonomic observation tube with normal field capable of tilting adjustment with an erect image and the eyepiece position can be adjusted by 45 mm toward the front and rear.

(Figs. 29 & 30) 1. Turn the condenser height adjustment knob @ to raise the condenser to its upper limit. 2. Focus on the specimen using the 10X objective. #When using the U-SC3 swing-out condenser, move the top lens into the light path. 3. Rotate the field iris diaphragm ring ² in the direction of the arrow so that the diaphragm image comes inside the field of view. 4. Manipulate the condenser height adjustment knob @ to focus on the diaphragm image 5. Turn the two condenser centering screws ³ to move the iris diaphragm image to the center of the field of view. 6. Gradually open the field iris diaphragm. The condenser is properly cen- tered if the iris image is centered and inscribed in the field of view. 7. During actual use, open the field diaphragm slightly until its image cir- cumscribes the field of view. Effects of Field Iris Diaphragm (Fig. 30) The field iris diaphragm restricts the diameter of the beam of light enter- ing the objective and thus excludes extraneous light, improving image contrast. The diameter of the field iris should e adjusted for objective power to the extent that it just circumscribes the field of view. (See “Com- patibility of Objectives and Condensers” on the next page.) Fig. 30

2X Applicable (FN 26.5) by moving top lens out of the light path.* (FN 26.5) 4X Applicable to FN 22 10-60X Applicable (FN 26.5) Applicable (FN 26.5) Engage top lens in light path (FN 26.5) 100X Applicable (FN 26.5) N.A. scale @ ² Objective pupil 70-80% 30-20% Aperture Iris Diaphragm (Figs. 31 & 32) · The aperture iris diaphragm determines the numerical aperture of the illumination system. Matching the numerical aperture of the illumination system with that of the objective provides better image resolution and contrast, and also increases the depth of focus. · Since the contrast of microscope specimens is ordinarily low, setting the condenser aperture iris diaphragm to between 70% and 80% of the N.A. of the objective in use is usually recommended. If necessary, adjust the ratio by removing the eyepiece and looking into the eyepiece sleeve while adjusting the aperture iris diaphragm ring @ until the image shown in Fig. 31 is seen. }Using the numerical aperture scale: Set the condenser numerical aperture scale to about 80% of the NA value ² of the respective objective. (Fig. 32) Example: With the UPlanFI40X (NA 0.75), set the scale to 0.75 x 0.8 = 0.6. 2 Compatibility of Objectives and Condensers * When using the U-SC3 swing-out condenser together with a 1.25X to 4X objective, fully open the condenser aperture iris diaphragm and use the field iris diaphragm in the base as aperture diaphragm. With the 1.25X to 2X objectives, the surroundings of the field of view may become dark. }To obtain better illumination in photomicrography using a 1.25X to 4X objective, use of the U-ULC-2 is recommended.

Fig. 33 3-7 Objectives with Correction Collar Fig. 34 @ 1 Using Immersion Objectives (Fig. 33) 1. Focus on the specimen with a lower-power objective. 2. Place a drop of provided immersion oil onto the specimen at the area to be observed. 3. Turn the revolving nosepiece to engage the immersion objective, then focus using the fine adjustment knob. #Since air bubbles in the oil will affect the image quality, make sure that the oil is free of bubbles. a. To check for bubbles, remove the eyepiece and fully open the field and aperture iris diaphragms, then look at the exit pupil of the objective inside the observation tube. (The pupil should appear round and bright.) b. To remove bubbles, turn the revolving nosepiece to repeatedly defocus and refocus the oil immersion objective. }f the condenser engraving shows a numerical aperture (NA) of 1.0 or higher, the number applies only when oil is applied between the slide glass and the top surface of the condenser. When oil is not present, the NA is about 0.9. 4. After use, remove oil from the objective front lens by wiping with gauze slightly moistened with an ether (70%)/alcohol (30%) mixture. Caution in use of immersion oil If immersion oil enters your eyes or contacts with your skin, immediately take the following treatment. Eyes: Rinse with fresh water (for 15 minutes or more). Skin: Rinse with water and soap. If the appearance of the eyes or skin is altered or pain persists, immedi- ately see your doctor. }If the cover glass thickness is not 0.17 mm, the objectives cannot mani- fest their performances. If a correction collar equipped objective is used in this case, the difference in thickness can be compensated for by ad- justing the collar. Adjustment Procedure · If the cover glass thickness is known, set the correction collar @ to that value. (Fig. 34) · If the cover glass thickness is unknown, adjust the correction collar @ and fine adjustment knob alternately until the positioning with the high- est resolution is obtained. #Be careful not to touch the correction collar @ when turning the revolving nosepiece.

please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list, please contact your local Olympus representative for assistance. 1. Optical System 28 3 Problem Cause Remedy Page a) Bulb does not light. Bulb is burned out. Replace bulb. Power cord is unplugged. Plug power cord into the power outlet. Transmitted/reflected light switch is set to (reflected light). (TRF models only) Set the switch to (transmitted light illumination). b) Bulb operates, but field of view re- mains dark. Aperture and field iris diaphragms are not opened wide enough. Adjust them to proper sizes. Condenser is lowered too much. Adjust the condenser height position. Light path selector knob is set to the position. Move the knob to the or posi- tion. c) Field of view is obscured or not evenly illuminated Light path selector knob is in an inter- mediate position. Set the knob according to the obser- vation method. Revolving nosepiece is not correctly en- gaged. Make sure that the revolving nosepiece clicks properly into place. Condenser is not attached properly. Re-attach it. Revolving nosepiece is not attached properly. Push the side dovetail all the way until it is stopped. An objective that falls outside of the condenser’s illumination range is used. Use a condenser to match the pur- pose. Condenser is not properly centered. Center the condenser. Field iris diaphragm is stopped down too far. Open the field iris diaphragm until it circumscribes the field. Bulb is not mounted correctly. Push the pins of halogen bulb all the way until the stop position. d) Dirt or dust is visible in the field of view. Dirt/dust on the eyepiece Clean thoroughly. Dirt or the to surface of the condenser Dirt/dust on the specimen e) Visibility is poor. · Image is not poor. · Contrast is poor. · Details are indistinct. · Image glares. A non-UIS objective is used. Use only UIS series objectives with this microscope. Condenser is lowered too far. Adjust the condenser height position. Aperture iris diaphragm is stopped down too far. Open aperture iris diaphragm. Revolving nosepiece is not mounted properly. Push the slide dovetail all the way until it is stopped. Correction collar on correction collar equipped objective is not properly ad- justed. While focusing, turn the correction collar to find the best position. Front lens of objective is dirty. Clean objective. Immersion oil is not being used with an oil immersion objective. Use immersion oil. 18/19 18 16 16 28 19 18 18 28 18 19 –– 20 20 3 29 5 –– –– 26

20 –– Remove the bubbles. Recommended immersion oil is not used. Use the provided immersion oil. Dirt/dust on specimen. Clean it. Dirt/dust on condenser Inappropriate object side or cover glass thickness. Replace with glass of recommended thickness. f ) One side of image is blurred.. Objective is not correctly engaged in light path. Make sure that revolving nosepiece clicks into place correctly. Revolving nosepiece is not correctly mounted. Push slide dovetail all the way until it is stopped. Stage is not correctly mounted. Re-attach it. Specimen is not correctly mounted on stage. Place specimen correctly on top of stage and secure it with slide holder. g) Image appears to waver. Revolving nosepiece is not corrected mounted. Push slide dovetail all the way until it is stopped. Objective is not correctly engaged in light path. Make sure that revolving nosepiece clicks into place correctly. Condenser is not properly centered. Center the condenser. h) Field of view becomes only slightly brighter when the voltage is raised. Condenser is not properly centered. Center the condenser. Condenser is lowered too far. Adjust the condenser height position. 2. Electrical System a) Bulb intermittently lights and goes out. Bulb is nearly burned out.. Replace bulb. A connector is not properly connected. Check all connectors. b) Bulb burns out almost immediately. Wrong type of bulb is being used. Use correct bulb type. c) Brightness does not change when you turn light intensity adjustment knob. Light intensity preset button is set to ON. Press button to OFF. d) All voltage indicator LEDs light and voltage cannot be varied with light intensity adjustment knob. Bulb is not installed. Install bulb. Bulb is burned out. Replace bulb. Lamp socket is not connected. Connect lamp socket correctly. e) Lamp voltage cannot be varied with light intensity adjustment knob. Bulb is burned out. Replace bulb. 3. Coarse/Fine Adjustment a) Coarse adjustment knob is hard to turn. Tension adjustment ring is tightened ex- cessively. Loose ring. You are trying to raise stage without coarse adjustment knob while pre-focus- ing lever is kept locked. Unlock pre-focusing lever b) Stage drifts down by itself or focus is lost during observation. Tension adjustment ring is too loose. Tighten ring. 20 3 12 –– –– –– 12 –– –– 18 18 18 28 28 8 28 28 11 28 28 11 11 e) Visibility is poor. · Image is not poor. · Contrast is poor. · Details are indistinct. · Image glares.

When adjusting stage height, you forgot to reattach upper stopper screw. Reattach upper stopper screw. d) Coarse adjustment will not go all the way up. Pre-focusing lever is locked at a too low height. Unlock pre-focusing lever. e) Coarse adjustment will not go all the way down. Condenser holder is too low. Raise condenser holder. f ) Objective makes contact with specimen before focus is obtained. –– 11 Specimen is mounted upside down. Mount specimen correctly. 18 4. Observation Tube a) Field of view of one eye does not match that of the other. Interpupillary distance is incorrect. Adjust interpupillary distance. Incorrect diopter adjustment. Adjust diopter. Different eyepieces are used on left and right. Change on eyepiece to match the other so that both sides are the same type. Your view is not accustomed to micro- scope observation. Upon looking into eyepieces, try look- ing at overall field before concentrat- ing on specimen range. You may also find it helpful to look up and into dis- tance for a moment before looking back into microscope. 5. Stage a) Image shifts when you touch stage. Stage is not properly mounted. Clamp stage. b) Specimen stops midway on the X- axis traverse. Specimen is not correctly positioned. Place specimen correctly. c) Horizontal and/or X- and Y-axis knobs are too tight or too loose. Tension of horizontal and/or X- and Y- axis knobs is too high or too low. Adjust tension. d) Stroke has reduced. Stage guide is deviated. Use treatment method described on page 13. 15 15 –– –– 14 12 13 13 ––

UIS (Universal Infinity System) optical system 2. Illumination Built-in transmitted Koehler illumination 12V, 100W long-life halogen bulb (pre-centered) 12V100WHAL-L (PHILIPS 5761) or 12 V, 50 W long-life halogen bulb (pre-centered) 12V50WHAL-L (LIFE JC) (Average life time: Approximately 2,000 hr. when used as directed) Light intensity voltage range: 1.0 to 12.0 V DC (continuous) Light intensity preset button (voltage adjustment range: 1.0 to 12.0 V DC) Rated voltage: 100-120/220-240 V , 1.8/0.8 A, 50/60 Hz Power consumption: 140 W 3. Focusing Stage height movement by roller guide (rack & pinion) Stroke per rotation: 0.1 mm (fine), 17.8 mm (coarse) Full stroke range: 25 mm Upper limit stopper Tension adjustment on coarse focus adjustment knob. 4. Revolving nosepiece Type U-5RE-2 U-D6RE U-D7RE U-P6RE 5-position revolv- ing nosepiece Universal 6-posi- tion revolving nosepiece Universal 7-posi- tion revolving nosepiece Universal 6-posi- tion centered revolving nose- piece Installable mod- ules None Transmitted DIC prism, transmitted light analyzer 5. Observation tube Type U-BI30-2 U-TBI3 U-TR30-2 U-SWTR-2 Widefield binocu- lar tube Widefield trinocu- lar tube Widefield tilting binocular Super widefield trinocular Field No. 22 26.5 Tube inclination 30° 5°-35° continuous 30° Interpupillary distance adjust- ment 50 mm to 76 mm Light path selec- tor None 3 steps: @ BI 100% ² BI 20%, Photo 80% ³ Photo 100% 6. Stage Type U-SVRB-4 U-SVLB-4 Common-axis knobs on bottom right. Common-axis knobs on bottom left. Rectangular ceramic-coated, wire-driven stage Size 156 mm (D) x 191 mm (W) Movement mechanism X- and Y-axis knobs with adjustable tension. Movement range: 52 mm in vertical (Y) direction, 76 mm in horizontal (X) direction. Specimen hold- ers (single slide holder) U-HLS4 U-HLST4 U-HRS4 U-HRST4 Specimen hold- ers (double slide holder) U-HLD4 U-HLDT4 U-HRD4 U-HRDT4

1.40 Aperture iris dia- phragm With numerical aperture scale Objective range 4X (for FN 22 widefield), 10X - 100X (for FN 26.5 super widefield) 1.25X (for FN 22 widefield), 2X - 100X (for FN 26.5 super widefield) 10X - 100X (for FN 26.5 super widefield) 8. Operating environment · Indoor use. · Altitude: Max. 2000 meters · Ambient temperature: 5° to 40°C (41° to 104° F) · Maximum relative humidity: 80% for temperatures up to 31°C (88°F), decreasing linearly through 70% at 34°C (93°F), 60% at 37°C (99°F), to 50% relative humidity at 40°C (104°F). · Supply voltage fluctuations; Not to exceed ±10% of the normal voltage. · Pollution degree: 2 (in accordance with IEC664) · Installation/Overvoltage category: II (in accordance with IEC664)

binations of eyepieces and objectives. The figure on the right shows the performance data engraved on the objectives. Name Numerical aperture NOTE Magnification Mechanical tube length Cover glass thickness Refer to the latest catalogue or consult your local Olympus representative for the updated information on the eyepieces and objectives that can be combined with this microscope. Indicates the objective is provided with a correction collar. Optical character Magni- fication N.A. W.D. (mm) Cover glass thick- ness Reso- lution (µm) Eyepiece Remark Objective WH10X (FN22) WH15X (FN14) Total mag. Depth of focus (µm) Field of view Total mag. Depth of focus (µm) Field of view Ach/Ach-P Achromat/Achromat for Polarized Light (FN 22) · 60X and 100XOI are only available with Ach. Iris Plan/Plan-P Plan Achromat/ Plan Achromat for Polarized Light (FN 22) · Plan-P has 4X only. UPlan-Fl/UPlan Fl-P Universal Semi- Apochromat/ Universal Plan Semi-Apochromat for Polarized Light (FN26.5) · 60X OI and 100X OI are only available with UPlan Fl. UPlan Apo Universal Plan Apochromat (FN26.5) Plan Apo Plan Apochromat (FN26.5) 10X 0.25 6.1 — 1.34 100X 28.0 2.2 150X 20.9 1.4 20X 0.40 3.0 — 0.84 200X 6.09 1.1 300X 4.64 0.7 40X 0.65 0.45 0.17 0.52 400X 3.04 0.55 600X 2.35 0.35 60X 0.80 0.15 0.17 0.42 600X 1.76 0.37 900X 1.39 0.23 100XO 1.25 0.13 — 0.27 1000X 0.69 0.22 1500X 0.55 0.14 100XOI 0.60-1.25 0.13 - 0.27 1000X 0.69 0.22 1500X 0.55 0.14 4X 0.10 22.0 — 3.36 40X 175 5.5 60X 85.8 3.5 10X 0.25 10.5 — 1.34 100X 28.0 2.2 150X 20.9 1.4 10XCY 0.25 10.5 — 1.34 100X 28.0 2.2 150X 20.9 1.4 20X 0.40 1.2 0.17 0.84 200X 6.09 1.1 300X 4.64 0.7 40X 0.65 0.56 0.17 0.52 400X 3.04 0.55 600X 2.35 0.35 50XOI 0.50-0.90 0.20 — 0.37 500X 1.75 0.44 750X 1.30 0.28 100XO 1.25 0.15 — 0.27 1000X 0.69 0.22 1500X 0.55 0.14 4X 0.13 17.0 — 2.58 40X 127 5.5 60X 92.9 3.5 10X 0.30 10.0 — 1.12 100X 22.4 2.2 150X 16.5 1.4 20X 0.50 1.6 0.17 0.67 200X 7.00 1.1 300X 5.22 0.7 40X 0.75 0.51 0.17 0.45 400X 2.52 0.55 600X 1.93 0.35 60XOI 0.65-1.25 0.10 0.17 0.27 600X 0.98 0.37 900X 0.60 0.23 100XO 1.30 0.10 0.17 0.26 1000X 0.66 0.22 1500X 0.52 0.14 100XOI 0.60-1.30 0.10 0.17 0.26 1000X 0.66 0.22 1500X 0.52 0.14 4X 0.16 13.0 — 2.1 40X 99.5 5.5 60X 71.7 3.5 10X 0.40 3.1 0.17 0.84 100X 15.9 2.2 150X 11.5 1.4 20X 0.70 0.65 0.17 0.48 200X 4.65 1.1 300X 3.39 0.7 40X 0.85 0.2 0.11-0.23 0.39 400X 2.14 0.55 600X 1.62 0.35 40XOI 0.5-1.00 0.12 - 0.34 400X 1.70 0.55 600X 1.30 0.35 60X 0.90 0.20 0.11-0.23 0.37 600X 1.50 0.37 900X 1.17 0.23 100XOI 0.5-1.35 0.10 0.17 0.25 1000X 0.62 0.22 1500X 0.49 0.14 1.25X 0.04 5.1 — 8.38 12.5X 872 17.6 18.75X 639 11.2 2X 0.08 6.0 — 4.19 20X 398 11.0 30X 287 7.0 40X 0.95 0.14 0.11-0.23 0.35 400X 1.86 0.55 600X 1.40 0.35 60XO 1.40 0.10 0.17 0.24 600X 0.85 0.37 900X 0.64 0.23 100XO 1.40 0.10 0.17 0.24 1000X 0.59 0.22 1500X 0.47 0.14 UMPlan Fl No Cover Universal Plan Semi-Apochromat (FN26.5) 40X 0.75 0.63 0 0.45 400X 1.66 0.55 600X 1.27 0.35 ND filter Iris Iris Iris Iris Iris Iris ND filter UApo Universal Apochromat (FN22) MPlan Apo No Cover Plan Apochromat (FN26.5) Plan Fl Plan Semi-Apochromat (FN26.5) UPlan Apo-W Universal Plan Apochromat (Water- Immersion) (FN26.5) 100XO 1.40 0.10 0 0.24 1000X 0.59 0.22 1500X 0.47 0.14 10XCY 0.30 9.90 — 1.12 100X 22.0 2.2 150X 16.4 1.4 100X 0.95 0.20 0.14-0.20 0.35 1000X 1.0 0.22 1500X 0.83 0.14 20X/340 0.75 0.55 0.17 0.45 200X 4.3 1.1 300X 3.10 1.4 20XW/340 0.70 0.40 0.17 0.48 200X 4.08 1.1 300X 3.37 1.4 40X/340 0.90 0.20 0.11-0.23 0.37 400X 2.0 0.55 600X 1.50 0.35 40XW/340 1.15 0.26 0.13-0.25 0.29 400X 1.29 0.55 600X 1.08 0.35 40XOI/340 0.65-1.35 0.10 0.17 0.25 400X 1.2 0.55 600X 0.88 0.35 Iris 10XW 0.40 0.50 0.17 0.84 100X 14.0 2.2 150X 11.4 1.4 60XW 1.20 0.25 0.15-0.20 0.28 600X 0.90 0.37 900X 0.78 0.23

assembly. The BX51TRF and BX52TRF microscope frames are assembled in the same procedures as the BX51TF and BX52TF frames. The module numbers shown in the following diagram are merely the typical examples. For the modules with which the module numbers are not given, please consult your Olympus representative or the catalogues. #When assembling the microscope, make sure that all parts are free of dust and dirt, and avoid scratching any parts or touching glass surfaces. Assembly steps enclosed in will be detailed on the subsequent pages. }Most assembly operations are possible by using the Allen screwdriver ( ) provided with the microscope. However, the assembly of the reflected light illuminator requires the use of the provided Allen wrench ( ) for clamping the internal screws (to ensure the performance, we recommend that you have your Olympus representative assemble or remove this module). ASSEMBLY 7-1 Assembly Diagram Eyepiece WH10X (FN 22) 35WH10X (FN 22) SWH10X-H (FN 26.5) 35SWH10X (FN 26.5) Observation tube U-BI30-2 (FN 22) U-TR30-2 (FN 22) U-TBI3 (FN 22) U-SWTR-2 (FN 26.5) U-ETBI (FN 22) Intermediate attachment U-EPA2 U-DO3 U-TRU, etc. Tube clamping screw Revolving nosepiece U-5RE-2 U-D6RE U-D7RE U-P6RE Specimen holder clamping knobs Specimen holder U-HLD-4 U-HRD-4 U-HLS-4 U-HRS-4 UIS series objective Stage U-SVRB-4 U-SVLB-4 U-SRG Stage clamping knob Condenser U-AC2 U-SC3 U-AAC U-ULC-2 U-UCD8 Reflected light illuminator BX-URA2 BX-RFA Microscope frame BX51TRF BX52TRF Power cord 100 W halogen bulb 12V100WHAL-L 12V50WHAL-L 100 W halogen lamp housing U-LH100-3 Lamp socket clamping screw Microscope frame BX51TF BX52TF Condenser clamping knob Reflected lamp housing Revolving nosepiece clamping screw

Fig. 35 Fig. 36 Fig. 38 7-2 Detailed Assembly Procedures @ ² ³ ³ @ ² 1 Installing the Bulb (Figs. 35 - 37) }Use only the designated bulb 12V100WHAL-L (PHILIPS 7724) or 12V50WHAL-L (LIFE JC). 1. Fully loosen the lamp housing clamping screw @ on to of the lamp housing cover with the provided Allen screwdriver. 2. Lift the lamp housing cover ²upward to remove it. 3. Turn the lamp socket ³ by 90° in the direction indicated by the arrow. 4. Holding the bulb ƒ with gloves or a piece of gauze, depress the bulb clamping lever | and insert the bulb pins … fully into the pin holes † on the lamp socket. Gently release the bulb clamping lever to the original position to secure the bulb. To prevent reduced bulb life or cracking, do not touch the bulb with bare hands. If fingerprints are accidentally left on the bulb, wipe the bulb with a soft cloth. 5. Side the lamp housing cover onto the housing base from the above. Tighten the clamping screw @ while pressing downward on the cover. (Fig. 35) The bulb and the lamp socket are areas near these will be extremely hot during and right after use. Set the main switch to “ ” (OFF), disconnect the power cord from the wall outlet, then allow the old bulb and lamp socket to cool before replacing the bulb with a new of the designated type. 8 Attaching the Condenser (Fig. 38) 1. Turn the coarse adjustment knob @ to raise the stage to its upper limit. 2. Turn the condenser height adjustment knob ² to lower the condenser holder to its lowest position. 3. Fully loosen the condenser clamping knob ³. 4. Position the condenser so that the aperture scale is located in front. Gently slide the condenser along the dovetail all the way until it is stopped. }When attaching the U-SC3 swing-out condenser, align the positioning pin at the back of the condenser with the groove in the condenser sleeve. #When attaching a condenser with swing-out top lens, swing out the top lens out of the way before inserting the condenser. 5. Tighten the condenser clamping knob and gently raise the condenser holder to its highest position. Fig. 37 | … ƒ †

to excessive force. Make sure that the main switch @ is set to “ ” (OFF) before con- necting the power cord. Always use the power cord provided by Olympus. IF no power cord is provided with the microscope, please select the proper power cord by referring to section “PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of this instruction manual. 1. Connect the power cord plug ² to the AC receptacle ³. The power cord should be connected to a grounded, 3-conductor power outlet. If the power outlet is not grounded properly, Olympus can no longer warrant the electrical safety performance of the equip- ment. 2. Plug the power cord plug | into the wall outlet ƒ. Should the power cord come in contact with the hot lamp housing, the power cord could melt and cause electric shock. Be sure to route the power cord away from the lamp housing. Fig. 38 Fig. 39 Fig. 40 @ ² @ ² ³ | ƒ 9 Attaching the Eyepiece (Fig. 39) #When using the U-BI30-2 binocular tube, eyepieces with built-in mi- crometer disk cannot be attached. #When using a finder eyepiece or an eyepiece with focusing mirror, attach it to the right-hand eyepiece sleeve. When doing so, make sure that the eyepiece positioning pin @ fits into the notch ² on the eyepiece sleeve. #The super-widefield trinocular observation tube is equipped with a positioning notch on both eyepiece sleeves. Make sure that the positioning pins on both eyepieces fits into the respective notches. Attaching the Power Cord (Figs. 40 & 41) 10

If no power supply cord is provided, please select the proper power supply cord for the eqipment by referring to “ Specifications ” and “ Certified Cord ” below: CAUTION: In case you use a non-approved power supply cord for Olympus products, Olympus can no longer warrant the electrical safety of the eqipment. Specifications Voltage Rating Current Rating Temperature Rating Length Fittings Configuration 125V AC (for 100-120V AC area) or, 250V AC (for 220-240V AC area) 6A minimum 60°C minimum 3.05 m maximum Grounding type attachment pulg cap. Opposite terminates in molded-on IEC con- figuration appliance coupling. Table 1 Certified Cord A power supply cord should be certified by one of the agencies listed in Table 1 , or comprised of cordage marked with an agency marking per Table 1 or marked per Table 2. The fittings are to be marked with at least one of agencies listed in Table 1. In case you are unable to buy locally in your country the power supply cord which is equivalent and authorized agencies in your country. Country Agency Certification Mark Country Agency Certification Mark Argentina Australia Austria Belgium Canada Denmark Finland France Germany Ireland IRAM SAA ÖVE CEBEC CSA DEMKO FEI UTE VDE NSAI Italy Japan Netherlands Norway Spain Sweden Switzerland United Kingdom U.S.A. IMQ MITI KEMA NEMKO AEE SEMKO SEV ASTA BSI UL

APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS Approval Organization Printed or embossed Harmoniza- tion Marking (May be located on jacket or insulation of internal wir- ing) Alternative Marking Utilizing Black-Red-Yellow Thread (Length of color section in mm) Black Red Yellow Comite Electrotechnique Belge (CEBEC) Verband Deutscher Elektrotechniker (VDE) e.V. Prüfstelle Union Technique de d´Electricite´ (UTE) Instituto Italiano del Marchio di Qualita´ (IMQ) British Approvals Service for Electric Cables (BASEC) N.V. KEMA SEMKO AB Svenska Elektriska Materielkontorollanstalter Österreichischer Verband für Elektrotechnik (ÖVK) Danmarks Elektriske Materielkontrol (DEMKO) National Standards Authority of Ireland (NSAI) Norges Elektriske Materiellkontroll (NEMKO) Asociacion Electrotecnica Y Electronica Espanola (AEE) Hellenic Organization for Standardization (ELOT) Instituto Portugues da Qualidade (IPQ) Schweizerischer Elektro Technischer Verein (SEV) Elektriska Inspektoratet CEVEC USE IEMMEQU BASEC KEMA-KEUR SEMKO <ÖVE> NEMKO ELOT l np l SEV SETI 10 30 10 30 10 10 30 30 10 10 30 50 10 10 30 10 30 30 10 10 50 30 10 50 30 10 30 30 30 50 10 10 70 30 10 70 30 30 70 10 10 90 10 30 90 10 30 90 Underwriters Laboratories Inc. (UL) SV, SVT, SJ or SJT, 3 X 18AWG Canadian Standards Association (CSA) SV, SVT, SJ or SJT, 3 X 18AWG This device complies with the requirements of both directive 89/336/EEC concerning electromagnetic compatibility and directive 73/23/EEC concerning low voltage. The CE marking indicates compliance with the above directives.

Printed in Japan 2000 03 M 070–@ 2-43-2,Hatagaya, Shibuya-ku, Tokyo, Japan Postfach 10 49 08, 20034, Hamburg, Germany 2 Corporate Center Drive, Melville, NY 11747-3157, U.S.A. 491B River Valley Road, #12-01/04 Valley Point Office Tower, Singapore 248373 2-8 Honduras Street, London EC1Y OTX, United Kingdom. 104 Ferntree Gully Road, Oakleigh, Victoria, 3166, Australia