AmScope B100 Operation Instruction Manual

Operation Instruction To ensure the safety and obtain satisfactory performance, please study this operation instruction thoroughly before your operation.

16 Contents 1.Purpose……………………………………………………………………………2 2.Specifications………………………………………………………………………2 3.Installation…………………………………………………………………………4 4.Operation……………………………………………………………………………4 5.Maintenance…………………………………………………………………………8 6.Troubleshooting……………………………………………………………………10 7.Outfits……………………………………………………………………………13

Quadplex Nosepiece Objective Clamp Condenser Up- down Knob Coaxial Coarse And Electric Base Eyepiece Main Body Stage Stage Moving Konb Condenser Handle of Iris Aperture Power Switch And Light Collector Fig.1 B200 Binocular Biological Microscope Fine Focusing Knob Light Controller Condenser Up- down Knob Coaxial Coarse And Electric Base Eyepiece Nosepiece Objective Fine Focusing Knob Monocular Head Main Body Clamp Stage Condenser Light Controller Power Switch And Light Controller Fig.2 B100 Monocular Biological Microscope

microscope with original style, steady structure, convenient operation and clear image is suitable for observing various biological specimens, and mostly applied in school, college, hospital and family. 2. Specifications 2.1 Total magnifications Objective Total Magnifications Eyepiece 4X 10X 40X 100X 10X 40X 100X 400X 1000X 16X 64X 160X 640X 1600X 2.2 Objectives Objective Numerical Aperture (N.A.) Objective Field Resolving Power Working Distance Field Number ф18 Field Number ф20 Achromatic Objective Plan Objective 4X 0.10 4.5mm 5mm 2.8µm 13mm 16mm 10X 0.25 1.8mm 2mm 1.1µm 6.3mm 2mm 40X (S) 0.65 0.45mm 0.5mm 0.42µm 0.44mm 0.66mm 100X (Oil) (S) 1.25 0.18mm 0.2mm 0.22µm 0.3mm 0.42mm

2.3.1 Mechanical tube length: 160mm 2.3.2 Conjugate distance: 195mm 2.3.3 Head: Monocular, Inclined 30°, Rotable 360°, Eyepiece can be fixed, Anti-fungal systems. Seidentopf binocular head 30°, Rotable 360°, Interpupillary adjustable distance is 55-75mm, Diopter adjustable range ±5, Anti-fungal systems. 2.3.4 Nosepiece: Quadplex nosepiece 2.3.5 Mechanical stage: Size 142mm×134mm X-Y travel 50mm×70mm 2.3.6 Focusing systems: Coaxial coarse and fine focusing knobs, Coarse stroke 22mm, Fine division 2µm, Condenser up-down range 25mm 2.3.7 Condenser: Abbe condenser, N.A. 1.25, Adjustable aperture, Aperture center is adjustable. 2.3.8 Mirror: Mirror diameterф52mm (optional) 2.3.9 Filter: Built in blue filer, ф38.5mm 2.3.10 Anti-fungal systems: Eyepiece and objective is anti-fungal, anti-fungal system built in head. 2.3.11 Electric components: Input voltage AC85-265V, 50/60Hz Output voltage DC1.2-6V 6V/20W halogen lamp

Fuse 2A ф5×20 3. Installation B series microscope is packed integrate, and it is convenient for installation. Open the package carton, and insert eyepiece into the eyepiece tube of the head (eyepiece for monocular microscope has been inserted before packing). If objectives don’t be fixed, please take objectives out from objectives packing box, and drive them into the holes of nosepiece orderly and tightly according to the times. Take out the oil bottle and dust cover, and place it properly for convenient use. 4. Operation 4.1 Electric components Make sure the supply voltage meets the instrument’s requirement, then plug in it. Rotation potentiometer with power switch used by the instrument is for protecting lamp. Mirror Bracket Mirror Base Fig.4 Mirror Clamp HandleSpecimen Clamp Condenser Vertical Moving Knob Horizontal Moving Knob Handle of Iris Aperture Condenser Up- down Knob Fig.3 Stage And Condenser

Please place specimen on the stage, and the face with cover glass should be up, then clip it with clamp. Clipping specimen should be careful in order to avoid spoiling glass. Place specimen on the stage levelly, and make it into optical path by adjusting vertical moving knob and horizontal moving knob. 4.3 Condenser (Shown in Fig.3) 4.3.1 Condenser up-down Turn the condenser up-down knob to adjust the distance between condenser front and the specimen, and change the equal illumination to obtain the best brightness. 4.3.2 Iris aperture adjustment Stir the handle of iris aperture of condenser to adjust size of iris aperture for adjusting the contrast of the specimen. 4.4 Interpupillary distance and diopter adjustment (Shown in Fig.5) 4.4.1 Interpupillar distance is different for everyone, so interpupillary distance should be adjusted before using binocular microscope. Please take the two eyepiece tubes to turn until the bright rings observed by two eyes are in superposition entirely. ▲B100 monocular microscope don’t need to be adjusted. 4.4.2 Diopter adjustment As focusing for binocular, user should observe right eyepiece with right eye, and make the right eyepiece clear by focusing adjustment, then observe the left eyepiece, at the same time, adjust the diopter ring of the left eyepiece tube to make the image of Diopter Ring Eyepiece Interpupillary Distance Scale Fig.5 Interpupillary Distance Adjustment And Diopter Ring Eyepiece Tube

4.5 Focusing adjustment Usually 10X objective should be used for focusing adjustment first. Take 10X objective into bright path, then observe the specimen with right eyepiece by right eye and turn coarse focusing knob to find the image, then turn slowly fine focusing knob to make the image clear. All objectives of our microscope are precisely adjusted, and approximate clear image should be found with the other objectives after getting the clear image with 10X objective. If the image isn’t enough clear with the other objectives, please turn fine focusing knob slightly to find the satisfactory image. ▲Don’t turn left and right coarse and fine focusing knobs with different direction with power at the same time, if so, the focusing system will be damaged. ▲Don’t directly pull objective to turn nosepiece when user changes the different objectives, if so, optical quality of microscope possibly be affected. The right way is to take the tooth-like part of the nosepiece to turn it, and make the objective into correct position and into the bright path. 4.6 Usage of the tension adjustment ring (Shown in Fig.6) The tension adjustment ring can adjust the tension of the coarse and fine focusing unit to prevent the stage from sliding down automatically and to improve the comfort Fig.6 Coarse A F F nd ine ocusing Left Coarse Left Fine Right Fine Right Coarse Tension Focusing Knob Focusing Knob Focusing Knob Adjustment Ring Focusing Knob Knobs And TensionAdjustment Ring

counterclockwise makes tension increase. 4.7 Usage of immersion oil objective The 100X objective of B series microscope can be used for observation even if without immersion oil. However, adding moderate immersion oil between the front lens of 100X objective and the cover glass of specimen can make the image clearer. Please pay attention that air bubble and impurity can’t be in the immersion oil, otherwise, the image would be affected. First of all, take 40X objective which has been focused well out from bright path, then take 100X objective into bright path. At this time, nosepiece or stage moving knob should be turned slightly, and also slightly turn fine focusing knob to clear away the air bubble of immersion oil, otherwise, the air bubble would affect the image badly. ▲After immersion oil used, the oil of specimen and the microscope surface should be soon cleaned by absorbent cotton, lens paper, gauze or soft cotton cloth with moderate mixture of pure industrial alcohol and ether (proportion 1:4) ▲Standard thickness 0.17mm cover glass should be chosen when high times objective used, and thickness error should be within 0.01mm, otherwise, image definition would be affected. 4.8 Mirror (Shown in Fig.4) Mirror of B series microscope is optional, and it is used when electrical power isn’t supplied. Mirror doesn’t be suggested as illumination source when 100X objective used.

reflecting face to the light source, and make reflecting face and horizontal face as 45° approximately to make the light to specimen as possible. The mirror has two sides, one is a plane mirror, and the other one is a concave one. Concave mirror is suitable for large-area outside light source, such as bright sky. 4.9 Points of attention after using 4.9.1 Power of microscope should be turned off after using, and the plug should be pulled down. If immersion oil used, please clean objective and specimen soon. Finally, cover microscope with dust cover. 4.9.2 Please take eyepiece and objectives out from microscope if user will stop using it for a long time, and place eyepiece and objectives into drier with drying agent. Cover microscope with dust cover. 5.Maintenance 5.1 Clean microscope 5.1.1 Don’t touch the lens with hand, Dust on lens should be cleaned by soft brush or absorbent cotton or cleaned by absorbent cotton, lens paper with the mixture of alcohol and ether (proportion 1:4). 5.1.2 Alcohol and ether all are burnt easily, please take them away from fire. Be careful for turn on and off power. 5.1.3 Don’t clean painted metal and galvanizing metal with organic solvent such as alcohol, ether or the mixture of the both. Silicon cloth or soft cleaning preparation is suggested to clean it.

5.2 Environment of using and placing 5.2.1 Microscope should be used and placed in a cool, dry, non-dust, non-shake and non-corrosive gases environment. 5.2.2 Microscope should be used in environment of indoor temperature 0°-40°C and maximum relative humidity 85%. 5.2.3 Removing humidity equipment is suggested to be installed when microscope used in heavy humidity area to avoid fungus and mist damage instrument. 5.2.4 Please pay attention to prevent microscope from violent shake and vibration in application and in carrying. Don’t drag it on the surface of worktable to avoid damage microscope and worktable. U screw Knurled n Lamp Adjustment Knurled Pull Over Bulb Housing Cover, Replace Bulb, Close Cover The Lamp Cover Thumb Screw Thumb Screw Fig.7 Replacement of Bulb 5.3 Replacement of bulb (Shown in Fig.7) 5.3.1 Turn off power, and pull out plug. 5.3.2 Wait the bulb become cool. ▲ Please be sure that the bulb is cool, then follow by the operations. 5.3.3 Lay aside the microscope reliably, unscrew the knurled thumb screw of the

5.3.4 Pull over the lamp housing cover. 5.3.5 Pull out the bulb should be replaced, hold a new bulb with silk cloth to avoid fingerprint and dust affect bulb brightness and service life, and insert fully the contact pins into the bulb socket. 5.3.6 Close the lamp housing cover, and screw the knurled thumb screw. ▲After working for above 10 hours continuously, better cut off the microscope about 30 minutes. 5.4 Replacement of fuse (Shown in Fig.8) 5.4.1 Cut off power of microscope, and pull out the plug. 5.4.2 Unscrew fuse cap in the back of base, take out old fuse. 5.4.3 Replace a new fuse, then screw the fuse cap. 5.5 Stop to use microscope, please cut off power, cover the dust cover, and place it in a cool and dry environment. 6.Troubleshooting In the period of using B series microscope, if there is any trouble occurs, please referring to the following sheet listing some common troubleshooting，and resolve them. Power Wire Fuse Cap Fig.8 Replacement of Fuse

Plug is unreliable Plug in again Bulb is broken Change bulb Fuse is broken Change fuse Bulb is flickering or brightness is unsteady Bulb is unstable Insert it again Bulb is broken Replacing bulb Brightness of view field isn’t enough or is uneven Bulb specification doesn’t meet the requirement Replacing bulb Brightness isn’t adjusted correctly Adjust rotation potentiometer Objective isn’t in correct position Make the objective in correct position The size of iris aperture is too small Adjust the size of iris aperture Lens (objective, eyepiece, condenser, light collector) has dust Clean it Position of condenser is too low Higher condenser Image isn’t clear Cover glass of specimen doesn’t meet the requirement Use required thickness cover glass (0.17mm) Cover glass of specimen isn’t in up direction Place specimen correctly

isn’t enough) Surface of objective lens is dirty (especially it is easy for the front lens of 40X objective to dip in immersion oil) Clean it Immersion oil isn’t used for 100X objective (oil) Use immersion oil Immersion oil doesn’t meet the requirement Use immersion oil supplied by us There is bubble in immersion oil Clear the bubble way Size of iris aperture isn’t proper Adjust the size of iris aperture Position of condenser is too low Readjust the position of condenser One side of image is dark or image is moving as focusing Objective isn’t in correct position Make the objective in correct position Specimen isn’t placed correctly Place specimen levelly on stage and clip it with clamp Objective touches specimen as changing low times objective to high times objective Cover glass of specimen isn’t in up direction Place specimen correctly Cover glass doesn’t meet the requirement Use required thickness cover glass (0.17mm) Image observed by two eyes aren’t in superposition entirely. Interpupilary distance isn’t adjusted correctly Adjust interpupilary distance according to two eyes It is easy for eyes to be tired during observing Diopter isn’t adjusted correctly Readjust diopter

Items Specification B102 B103 B104 B202 B203 B204 Order No. Eyepiece WF 10X-18mm ● ● ● ●● ●● ●● WF10 WF 16X-13mm ○ ○ ○ ○○ ○○ ○○ WF16 WF 10X-18mm (With Pointer) ○ ○ ○ ○ ○ ○ WF10P WF10X-18 mm (Reticule 0.1 mm) ○ ○ ○ ○ ○ ○ WF10R WF 5X-20 mm ○ ○ ○ ○○ ○○ ○○ WF5 Achromatic Objective 4X/0.10 ● ● ● ● ● B-A4 10X/0.25 ● ● ● ● ● B-A10 40X/0.65 (S) ● ● ● ● ● B-A40 100X/1.25(Oil)(S) ○ ○ ● ○ ● B-AO100 Plan Objective 4X/0.10 ○ ○ ○ ○ ● B-P4 10X/0.25 ○ ○ ○ ○ ● B-P10 40X/0.65 (S) ○ ○ ○ ○ ● B-P40 100X/1.25 (Oil) (S) ○ ○ ○ ○ ● B-PO100 Monocular Head Inclined 30°, Rotatable 360° ● ● ● Mo-1 Seidentopf Binocular Head Inclined 30°, Rotatable 360°, Interpupilary Distance: 55-75mm ● ● ● Bi-1 Nosepiece Quadplex, Coaxial Coarse and Fine Focusing Knobs ● ● ● ● ● ● N-4 Fixed Stage Clips ● ● ○ S-1 Attachable Mechanical Stage Travel: 30mm×50mm ○ ○ ● ○ ○ S-2 Mechanical Stage Travel: 50mm×70mm ● ○ ● ● S-Ma

Diaphragm) ● ○ CS Abbe N.A. 1.25 (Iris Diaphragm) ● ● ● ● ● Ca Dark Field Ring Available For 4X-40X Objective ○ ○ ○ ○ ○ D-1 Base Mirror Built In Blue Filter ○ ○ ○ ○ ○ ○ Mo Incandescent Lamp 110V/220V, 20W Spare Lamp:1pc Built In Blue Filter ● ○ E1 Halogen Lamp 6V 20W Wide Voltage: 85V-265V Spare Lamp: 1 pc Built In Blue Filter ● ● ● ● ● E2 LED Illumination Systems ○ ○ ○ ○ ○ ○ LED Fuse Spare Fuse: 2 pcs ● ● ● ● ● ● Note: “●”In Table Is Standard Attachment. “○” Is Optional Accessories Packing Size: 340mm X 243mm X 425mm Gross Weight: 7 kgs Net Weight: 5.5 kgs