Rainin Pipet-Lite Manual 

volume ranges from 0.1 µL to 20 mL Pipet-Lite 200 µL shown Pipet-Lite Magnetic Assist Pipette ™

LLC. Pipet-Lite pipettes are manufactured under U.S. Patent Nos. 5,614,153, 5,700,959, and 5,849,248. LTS Lite Tip Ejection System is protected by U.S. patents 6,168,761 B1 and 6,171,553 B1. Other national patents pending. Table of Contents Introduction..................................................................1 Setting Volume..............................................................2 Filter............................................................................3 Tip Selection and Mounting ............................................3 Tip Immersion Depth .....................................................4 Operation.....................................................................4 Pipetting Guidelines.......................................................5 Pre-Rinsing Recommended............................................5 Reverse-Mode Pipetting .................................................5 Pipetting Liquids of Varying Density.................................6 Temperature Considerations ...........................................6 Autoclaving ..................................................................6 Tip Ejector Arm Removal................................................7 Pipet-Lite Storage..........................................................7 Troubleshooting and Repairs..........................................8 Service, Calibration, and Repair......................................9 Specifications .............................................................10 Replacement Parts ......................................................11 Contacting RAININ............................................Back Page Figures Figure 1 Pipet-Lite Pipette........................................1 Figure 2 Filter Orientation.........................................3 Figure 3 Removing the Tip Ejector Arm......................7 Line Drawings Pipet-Lite 2 µL ................................................12 Pipet-Lite 10 µL ................................................12 Pipet-Lite 20 µL ................................................12 Pipet-Lite 100 µL ................................................12 Pipet-Lite 200 µL ................................................12 Pipet-Lite 1000 µL ................................................13 Pipet-Lite 2000 µL ................................................13 Pipet-Lite 5000 µL ................................................13 Pipet-Lite 10 mL ................................................13 Pipet-Lite 20 mL ................................................13

Plunger button / volume adjustment Tip ejector button Stainless steel tip ejector arm Volume lock Digital volume indicator Body: high-impact, low thermal conductivity polymer Shaft Disposable tip Introduction Pipet-Lite is an air-displacement pipette which incorporates major ergonomic improvements to reduce the risk of repeti- tive strain injury and pain – low force seals and lower spring forces contribute to a lighter feel. A magnet is used to help sense and hold the piston in the zero position, which reduces static force on the operator’s hand. In the models with LTS™ shafts, the patented LiteTouch™ Tip Ejection System reduces total pipetting forces by up to 70%. Tip ejector arm

shown at left below so the volume setting mechanism is unlocked and free to turn. 2. With the mechanism unlocked, orient Pipet-Lite so you are looking at the digital volume indicator, then rotate the plunger button to change volume – counter-clockwise to increase, and clockwise to decrease volume. The volume indicator is read from the top down. 2–20 µL: Black – µL. Red – tenths, hundredths of µL. 100–200 µL: All digits black – whole µL. 1000–5000 µL: Red – mL. Black – tenths, hundredths of mL. 10 mL: Red – mL. Black – tenths of mL 20 mL: Red – mL. Black – tenths of mL. 3. To eliminate errors due to mechanical backlash: when set- ting the desired volume, first turn the knob 1⁄3 turn above the desired volume. Then turn the knob slowly clockwise until the desired volume is displayed. Always dial down to the desired volume. 4. Turn the volume lock clockwise (see diagram above) to prevent accidental changes to the volume setting. Example volumes for the 200 µL model are shown below (note the intermediate setting at the right). 2 Red digits Black digits

Pipet-Lite Range (µL) Increment Volume Adjustable Recommended µL 2 µL 0 to 2 0.1 to 2 0.002 10 µL 0 to 10 0.5 to 10 0.02 20 µL 0 to 20 2 to 20 0.02 100 µL 0 to 100 10 to 100 0.2 200 µL 0 to 200 20 to 200 0.2 1000 µL 0 to 1,000 100 to 1,000 2.0 2000 µL 0 to 2,000 200 to 2,000 2.0 5000 µL 0 to 5,000 500 to 5,000 5.0 10 mL 0 to 10 mL 1 mL to 10 mL 20.0 20 mL 0 to 20 mL 2 mL to 20 mL 20.0 Filter Pipet-Lite 5000 µL, 10 mL, and 20 mL pipettes use a filter in the end of the shaft to help prevent liquid entering the shaft and contaminating the piston, should the plunger snap up during aspiration. Using such a filter is particularly important when pipetting large volumes. Replace the filter if it gets wet. Figure 2 Filter Orientation The 5000 µL and 10 mL pipettes use the same filter oriented as shown in the diagram above: 5000 µL: insert the small diameter into the shaft. 10 mL: insert the large diameter into the shaft. Filter part numbers are 6190-164 (pack of 100) and 6190- 165 (pack of 1000). The filter for 20 mL is a cylinder. Filter part numbers: 6190- 221 (pack of 100) and 6190-222 (pack of 500). Tip Selection and Mounting Always use RAININ tips with Pipet-Lite pipettes. Pipet-Lite pipettes are calibrated with RAININ tips, and performance to published specifications can only be guaranteed when RAIN- IN tips are used. To mount a tip, press the Pipet-Lite shaft into the end of the tip with light force. The tip will seal properly on the shaft with minimal force — do not use more force than is required. 3

The recommended depth for tip insertion into the sample for each Pipet-Lite volume is shown below. Pipet-Lite Volume Range Immersion Depth 2 µL 0.1 - 2 µL 1-2 mm 10 µL 0.5 - 10 µL 1-2 mm 20 µL 2 - 20 µL 2 - 3 mm 100 µL 10 - 100 µL 2 - 3 mm 200 µL 20 - 200 µL 2 - 4 mm 1000 µL 100 - 1000 µL 2 - 4 mm 2000 µL 200 - 2000 µL 2 - 6 mm 5000 µL 500 - 5000 µL 6 - 10 mm 10 mL 1 mL - 10 mL 6 - 10 mm 20 mL 2 mL - 20 mL 6 - 10 mm Tip immersion depth is critical. If these depths are exceeded, the volume measured may be inaccurate, possibly out of specification. Tip angle is also important. Hold the pipette within 20 degrees of vertical. Operation Before pipetting valuable samples, it is a good idea to prac- tice aspirating and dispensing water before pipetting with actual samples. 1. Set the desired volume as described on page 2. 2. Attach a new RAININ tip. Press the shaft into the tip with only sufficient force to make a good seal. 3. Press the plunger button to the FIRST STOP, and hold it in this position. The magnetic latch will help you sense and hold this position. 4. Holding Pipet-Lite vertically, place the tip into the sample to the proper depth and relax your thumb pressure on the plunger. The light piston spring will move the piston upward, aspirating sample. Do not let go of the plunger button, or the piston may snap up quickly, resulting in inaccurate measurement. 5. Pause briefly (longer for macrovolume pipettes) to ensure that the full volume of sample is drawn into the tip. 6. Withdraw the tip from the sample. If any liquid remains on the outside of the tip, wipe it carefully with a lint-free tis- sue, taking care not to touch the tip orifice.

vessel and press the plunger slowly, past the FIRST STOP, to blowout (bottom of stroke.) Wait: 1 second for 2–200 µL volumes, 1-2 seconds for 1000 µL and larger. (Longer for viscous solutions.) 2. Still holding the plunger, withdraw the tip, sliding it along the wall of the vessel. Release the plunger. 3. Press the tip ejector button lightly to discard the tip. Use a new tip for each sample to prevent carry-over. Repeat for the next pipetting cycle. Pipetting Guidelines Pipet-Lite pipettes incorporate several new features which enhance pipetting consistency. You should also maintain: 1. Consistent pickup and dispense rhythm. 2. Consistent speed and smoothness when pipetting. 3. Consistent pressure on the plunger button at the FIRST STOP. 4. Consistent immersion depth. See table on page 4. 5. Pipette vertically, or within 20° of vertical. 6. Don’t invert or lay the pipette flat with liquid in the tip. Pre-Rinsing Recommended Some solutions may leave a film on the inside tip wall. This film remains relatively constant in successive pipettings with the same tip, so excellent precision can be obtained by re- filling the tip and using the refilled volume as the sample. Successive samples from this same tip will exhibit good reproducibility. Reverse Mode Pipetting Another way of reducing error due to film retention, especially useful for more viscous liquids, is reverse mode pipetting. The operating sequence is reversed: 1. Mount a disposable tip on the pipette shaft. 2. Press the pushbutton fully to the SECOND STOP. 3. Immerse the tip in liquid and allow the button to return slowly to the fully UP position. Wait a moment for the liq- uid column to reach equilibrium in the tip. 5

touching the orifice. 5. To dispense, rest the end of the tip against the vessel wall and press the plunger to the first stop. Hold this position a few seconds, or long enough for the liquid column to reach equilibrium again. 6. Remove the tip from the receiving vessel without blowing out the remaining liquid. 7. Return excess sample in the tip to the original sample container, if desired. Discard the used tip. Pipetting Liquids of Varying Density Pipet-Lite lets you compensate for solutions of density much different from water, by setting the volume slightly higher or lower than that required. The compensation amount must be determined empirically. For example, if pipetting 10 µL of CsCl solution, you deter- mine that the volume delivered is actually 8.5 µL (≥5 sam- ples). Change the volume setting to 11.8 µL and repeat the measurements. If the volumes delivered are still not close enough to 10 µL, make another slight volume adjustment until the measurements are as desired. Temperature Considerations Warm or cold liquids can be measured with good precision by using a consistent pipetting rhythm. This will help mini- mize any differences in heating or cooling effects within the pipette. Use a new tip each time for best accuracy and precision when measuring samples with temperatures greatly different from ambient, and do not pre-rinse. As with any air-displace- ment pipette, you will get best results if there is no delay between picking up the sample and dispensing it. Autoclaving Autoclavable parts of Pipet-Lite are the shaft and the tip ejector: 121°C, 1 bar, 15-20 minutes. Do not autoclave the complete pipette or any parts other than the shaft and the tip ejector.

Three types of tip ejector are used and all types can be removed with minimum effort - do not use force. For models up to 2000 µL, press in the quick-release tabs on the ejector arm and pull the ejector down. For 5000 µL and 10 mL models, grasp the top of the ejector arm and pull outward then downward. For the 20 mL model, pull off the lower part of the ejector arm; the upper part stays in place. (Replace by aligning the rod with the hole in the upper part and pressing firmly.) To replace the ejector arm (except 20 mL), insert the shaft through the large opening, align the top with the tip ejector pushrod, and push until the ejector arm snaps in place. Pipet-Lite Storage After use, store the pipette in a clean safe place. Pipet-Lite is a precision instrument and should be treated with the level of care appropriate for laboratory instrumentation. Three types of hanger are available to hold your Pipet-Lite conveniently when not in use. CR-7: Free-standing carousel holds seven pipettes. HU-M3: Set of three individual magnetic Hang-Ups™ for mounting on ferrous surfaces. HU-S3: Three Hang-Ups attached to a clamp which fits onto a shelf. 7 Figure 3 Removing the Tip Ejector Arm

WARNING: When removing the shaft from the pipette body, make sure the spring, seal and o-ring do not fall off the piston, espe- cially on the smaller models. Sample Splash (liquid inside the mechanism) 1. Remove the tip ejector arm. Also refer to the appropriate drawing on page 12 or 13. 2. On pipettes up to 1000 µL, unscrew the shaft coupling (B) and remove the shaft (C). For 2000 µL, unscrew the shaft. For 5000 µL, 10 mL and 20 mL unscrew the lower part of the shaft. Note the way the spring, seal and O-ring fit on the piston. 3. Inspect the seal assembly and piston for contamination. The piston should be shiny and free of corrosion. Clean with distilled water or isopropyl alcohol. Dry with a lint- free tissue and reassemble after inspecting the interior of the shaft for any contamination. For 5000 µL, 10 mL and 20 mL models, apply a small amount of grease to the seal. 4. If piston corrosion or staining is evident, do not use the instrument. Call 800-662-7027 for Service. Leaks, Inaccuracy, Abnormal Stroke 1. Loose shaft. Tighten coupling by hand. 2. Split or cracked shaft. Remove the tip ejector and inspect the shaft. Replace the shaft if necessary. If the shaft was dropped, remove it to see if the piston is bent. If so, return the instrument for service. 3. Worn seal and / or o-ring. All models up to 2000 µL incorporate a polyethylene seal and o-ring. Examine the seal and o-ring, replacing them as necessary. Pull off the old seal and o-ring, position the new seal and o-ring on the piston assembly as shown in the drawings on pages 12-13, and reassemble the pipette. Note: It is NOT necessary to recalibrate Pipet-Lite after changing seals. 4. Do not lubricate any components*. Pipet-Lite uses a dry sealing system. *Except 5000µL, 10 mL, and L-20ML models. These pipettes require a small amount of grease to be applied to the o-ring to make the seal. 8

After pipetting concentrated acids or highly corrosive solu- tions, disassemble Pipet-Lite and inspect and clean the pis- ton assembly, shaft, and seal with distilled water. Dry all components thoroughly and reassemble. Extensive contact with corrosive fumes may result in prema- ture seal wear and damage to the piston. Exposure of inter- nal components to corrosive fumes can be reduced by using RAININ tips with aerosol barrier filters. Service, Calibration and Repair RAININ Pipette Repair and Calibration facilities: California: 7500 Edgewater Drive, Oakland CA 94621 Tel. 800-662-7027, Fax 510-564-1683 Massachusetts: Rainin Road, Woburn, MA 01801 Tel. 800-662-7027, Fax 781-935-7631 Japan: 4-1-11, Bunkyo-Ku, Tokyo 113-0033 Tel. (03) 5689-8311, Fax (03) 5689-2670 METTLER TOLEDO Pipette Repair and Calibration facilities: Belgium: N.V. Mettler-Toledo s.a., B-1932 Zaventem Tel. (02) 334 02 11, Fax (02) 334 03 34 Germany: Mettler-Toledo GmbH, D-35353 Giessen Tel. (0641) 50 70, Fax (0641) 507 128 Denmark: Mettler-Toledo A/S, DK-2600 Glostrup Tel. (43) 270 800, Fax (43) 270 828 Spain: Mettler-Toledo S.A.E., E-08038 Barcelona Tel. (93) 223 76 00, Fax (93) 223 02 71 France: Mettler-Toledo s.a., F-78222 Viroflay Tel. (01) 309 717 17, Fax (01) 309 716 16 Italy: Mettler-Toledo S.p.A., I-20026 Novate Milanese Tel. (02) 333 321, Fax (02) 356 29 73 Netherlands: Mettler-Toledo B.V., NL-4004 JK Tiel Tel. (0344) 638 363, Fax (0344) 638 390 Sweden: Mettler-Toledo AB, S-12008 Stockholm Tel. (08) 702 50 00, Fax (08) 642 45 62 Service is also available in many other countries through authorized RAININ distributors. See www.rainin-global.com. Note: It is recommended to use only genuine RAININ replace- ment parts such as seals and shafts. It is NOT necessary to recalibrate the pipette after changing the seal or shaft. Recali- bration of the pipette is only necessary when the piston is replaced, and should be done only by qualified factory-trained personnel in one of the above-mentioned facilities.

lines when establishing your own performance specification in accordance with ISO 8655. Pipet-Lite Specifications Volume Increment Accuracy Precision Model µL µL % µL (±) % µL (≤) 2 µL 0.2 0.002 12.0 0.024 6.0 0.012 1.0 2.7 0.027 1.3 0.013 2.0 1.5 0.030 0.7 0.014 10 µL 1.0 0.02 2.5 0.025 1.2 0.012 5.0 1.5 0.075 0.6 0.03 10.0 1.0 0.1 0.4 0.04 20 µL 2 0.02 7.5 0.15 2.0 0.04 10 1.5 0.15 0.5 0.05 20 1.0 0.2 0.3 0.06 100 µL 10 0.2 3.5 0.35 1.0 0.1 50 0.8 0.4 0.24 0.12 100 0.8 0.8 0.15 0.15 200 µL 20 0.2 2.5 0.5 1.0 0.2 100 0.8 0.8 0.25 0.25 200 0.8 1.6 0.15 0.3 1000 µL 100 2 3.0 3.0 0.6 0.6 500 0.8 4.0 0.2 1.0 1000 0.8 8.0 0.15 1.5 2000 µL 200 2 3.0 6.0 0.6 1.2 1000 0.8 8.0 0.2 2.0 2000 0.8 16.0 0.12 2.4 5000 µL 500 5 2.4 12.0 0.6 3.0 2500 0.6 15.0 0.2 5.0 5000 0.6 30.0 0.16 8.0 10 mL 1 mL 20 5.0 50.0 0.6 6.0 5 mL 1.0 50.0 0.2 10.0 10 mL 0.8 80.0 0.16 16.0 20 mL 2 mL 20 5.0 100.0 0.6 12.0 10 mL 1.0 100.0 0.2 20.0 20 mL 0.8 160.0 0.16 32.0 Specifications are subject to change without notice.

Legend for Pipet-Lite 2 µL to 2000 µL A - Plunger Button B- Shaft Coupling C - Shaft* D - Tip Ejector* E - Piston Assembly F - Seal G - O-ring H - Stroke Spring J - Seal Retainer L- Series: L-2 L-10 L-20 L-100 L-200 L-1000 L-2000 A 6202-055 6202-056 6202-057 6202-058 6202-059 6202-060 6202-192 B 6202-062 6202-062 6202-062 6202-062 6202-062 6202-062 n/a C 6202-063 6202-064 6202-065 6202-066 6202-229 6202-230 6202-214 D 6202-071 6202-071 6202-071 6202-073 6202-231 6202-232 6200-168 E 6202-076 6202-077 6202-078 6202-079 6202-080 6202-081 6202-082 F 6200-131 6200-138 6200-143 6200-150 6200-154 6200-161 6200-166 G 6200-132 6200-139 6200-170 6200-151 6200-155 6200-162 6200-167 H 6202-083 6202-083 6202-083 6202-084 6202-084 n/a n/a J 6200-196 6200-196 6200-198 6200-201 6200-200 n/a n/a SL- Series: SL-2 SL-10 SL-20 SL-100 SL-200 SL-1000 SL-2000 A 6202-109 6202-110 6202-111 6202-112 6202-113 6202-114 6202-115 B 6202-062 6202-062 6202-062 6202-062 6202-062 6202-062 n/a C 6200-134 6200-140 6200-145 6200-147 6200-382 6200-383 6200-169 D 6200-133 6200-133 6200-144 6200-148 6200-231 6200-232 6200-168 E 6202-183 6202-184 6202-185 6202-079 6202-080 6202-081 6202-082 F 6200-131 6200-138 6200-143 6200-150 6200-154 6200-161 6200-166 G 6200-132 6200-139 6200-170 6200-151 6200-155 6200-162 6200-167 H 6202-083 6202-083 6202-083 6202-084 6202-084 n/a n/a J 6200-196 6200-196 6200-198 6200-201 6200-200 n/a n/a * These parts are autoclavable (C - shaft, D - tip ejector arm) Legend for Pipet-Lite 5000 µL , 10 mL and 20 mL A - Plunger Button C- Tip Ejector* D - Piston Assembly E - Piston O-ring F - Cylinder G - Cylinder O-ring H - Shaft* 11 L- Series: SL- Series: L-5000 L-10ML L-20ML SL-5000 SL-10ML A 6202-193 6202-194 6202-297 6202-217 6202-218 C 6200-373 6200-374 6202-298 6200-373 6200-374 D 6202-215 6202-216 6202-296 6202-215 6202-216 E 6200-363 6200-369 6202-299 6200-363 6200-369 F 6200-365 6200-371 6202-301 6200-365 6200-371 G 6200-364 6200-370 6202-300 6200-364 6200-370 H 6202-222 6202-223 6202-302 6200-362 6200-368 * These parts are autoclavable (C - tip ejector arm, H - shaft) Common parts for 5000 µL, 10 mL, and 20 mL: Tube of grease: 6100-555 Filters for 5000 µL and 10 mL: 6190-164 (pack of 100) 6190-165 (pack of 1000) for 20 mL: 6190-221 (pack of 100) 6190-222 (pack of 500)

Pipet-Lite 100 µL Pipet-Lite 200 µL A B C D E F G H J Pipet-Lite 20 µL A B C D E F G H J A B C D E F G H J A B C D E F G H J Legend for Pipet-Lite 2 µL to 2000 µL Models (this page and top of next page) A - Plunger Button B- Shaft Coupling C - Shaft D - Tip Ejector E - Piston Assembly F - Seal G - O-ring H - Stroke Spring J - Seal Retainer

Pipet-Lite 2000 µL A C D E F G A B C D E F G Pipet-Lite 5000 µL & 10 mL Pipet-Lite 20 mL A C H H D E F G A C D E F G Legend for Pipet-Lite 5000 µL, 10 mL and 20 mL (below) A - Plunger Button C- Tip Ejector Arm D - Piston Assembly E - Piston O-ring F- Cylinder G - Cylinder O-ring H - Shaft

Limited Warranty See the enclosed Limited Warranty and Limitations of Liability Statement. Please complete and return the Warranty Registra- tion Card on receipt of your pipette. RAININ pipettes are calibrated with RAININ tips. To assure excellent reproducibility and performance, use only RAININ tips as recommended in this manual. Specified performance is guaranteed only when RAININ tips are used. Contacting RAININ Technical Information: Phone: 800-543-4030 Fax: 781-938-1152 E-mail: [email protected] Pipette Service: Phone: 800-662-7027 Fax: 781-935-7631 E-mail: [email protected] Direct Order Line: Phone: 800-472-4646 Fax: 781-938-1152 E-mail: [email protected] Web: www.rainin.com From outside the U.S.: Massachusetts Office:001-781-935-3050 California Office: 001-510-564-1600 RAININ Japan: Phone: ++ 81 3 5689-8311 Fax: ++ 81 3 5689-2670 METTLER TOLEDO GmbH, Switzerland Phone: ++41 1 944 45 45 Fax: ++41 1 944 45 10 Other International Offices and Distributors: See www.rainin-global.com E-mail: [email protected] (from outside the U.S.) Web: www.rainin-global.com (from outside the U.S.) Rainin Instrument, LLC 7500 Edgewater Drive, Box 2160, Oakland, CA 94621-0060 a METTLER TOLEDO Company Prices and specifications are subject to change without notice. Copyright 2002, Rainin Instrument, LLC.