Rainin EDP3-Plus E8-10 Manual 

with LTS™ LiteTouch™ Tip Ejection ™ EDP3-Plus all-g uide s.co m

EDP3-Plus multichannel pipettes are based on EDP3-Plus single-channel models which use the patented LTS LiteTouch Tip Ejection System. Four versions of the multichannel EDP3-Plus are available: 8-channel (E8), 12-channel (E12), 16-channel in two rows (E2X8), and 24-channel in two rows (E24). 8 and 12-channel models are available in 6 volume ranges: 0.5-10 µL, 2-20 µL, 5-50 µL, 20-200 µL, 20-300 µL., and 100-1200 µL.The 2x8-channel and 24-channel models are available in 2 volume ranges, 2-20 µL and 10-100 µL. This is an addendum to the EDP3-Plus manual (9920-235) which describes pipette operation, modes, and options. Speed Setting The maximum speed setting for E8 and E12 multichannel pipettes is 10 for the 10-300 µL models, and 9 for the 1200 µL model. The maximum speed setting for E2X8 and E24 pipettes is 10. DISPLAY 4-KEY PAD DUAL TRIGGERS TIP EJECTOR BUTTON LIQUID END MANIFOLD LTS SHAFT NOZZLES LTS DISPOSABLE TIPS SHAFT COUPLING FIGURE 1 E8-200 PIPETTE

E8-10, E8-20, E12-10, E12-20, E2X8-20 & E24-20: 20µL LTS tips E8-50, E12-50, E8-200, E12-200, E2X8-100 & E24-100: 250 µL LTS tips E8-300 and E12-300: 300 µL LTS tips E8-1200 and E12-1200 use 1200 µL LTS tips (recommended) OR 1000 µL LTS tips – picks up 1200 µL (except aerosol-resistant) LTS tips have a cylindrical cross-section with a well-defined seal ring, thin wall, and positive stop. They seal properly on the shaft and cannot be jammed or forced too far onto the shaft nozzles. Mounting LTS Tips: 8- and 12-channel Mounting racked LTS tips onto E8 and E12 pipettes is simple. First align the shaft nozzles into the row of tips, holding the pipette at a slight angle. (Figure 2.1). Position the pipette upright and press the nozzles into the tips until the “positive stop” is reached. (Figure 2.2). The tips are now mounted with proper sealing. FIGURE 2: MOUNTING RACKED LTS TIPS, 8-AND 12-CHANNEL You do not need hand-tighten, use heavy pressure, or “rock” the nozzles onto the tips to obtain a good seal. Mounting LTS Tips: 2x8- and 24-channel 1. Align the first row of shaft nozzles with the first row of tips, holding the pipette almost vertically. Move the pipette as shown by the arrow to align the second row of tips. You may need to “rock” or rotate the pipette to get all tips aligned. 1 2 FIGURE 3: MOUNTING RACKED LTS TIPS, DOUBLE-ROW MODELS E2X8-20 E24-20 E2X8-100 E24-100 TIP RACK TIP RACK

the tips, lightly press both rows of shaft nozzles into the tips until you feel the “positive stops” in the tips. 3. While maintaining slight pressure, “rock” the pipette back and forth and in a circular direction to ensure both rows of tips are attached. Do not press very hard; a little practice will help you find the optimal pressure and amount of rocking. The tips are now mounted with proper sealing. Tip Immersion Depth The recommended depth for tip insertion into the sample for each EDP3-Plus multichannel model is shown in the table below. EDP3-Plus Model Immersion Depth E8-10, E12-10 2 - 3 mm E8-20, E12-20, E8-50, E12-50, E2X8-20, E24-20 2 - 3 mm E8-200, E12-200, E2X8-100, E24-100 2 - 4 mm E8-300, E12-300 2 - 4 mm E8-1200, E12-1200 4 - 10 mm Tip immersion depth is important. If exceeded, the volume mea- sured may be inaccurate, possibly out of specification. Tip angle: Operate the pipette within 20 degrees of vertical. Tip Ejection Simply press on the tip ejector button. All tips are ejected clean- ly with minimal pressure on the thumb. Positioning the Liquid End Manifold The liquid end manifold can be rotated to any angle for conve- nience. You do not need to loosen the coupling nut. AutoPace (AUTO) AutoPace allows multiple aliquots of the same volume to be dis- pensed repeatedly at equal time intervals after the dispense trig- ger is pressed once. For example, on the 300 µL volume pipette, all channels can dispense ten 30 µL aliquots at 2-second intervals with one touch of the trigger. In MULTI mode, press and hold the mode key to reach the options menu. Then click the mode key until “auto” appears on the dis- play. The first time used, the display will flash “off”. Use the arrow keys to set the time interval between dispenses. The range is 0.2 seconds to 25 seconds, in 0.1 second increments.

When the time interval is set, press either trigger to pick up the set volume. Then press the trigger again to begin dispensing automatically at the set time interval. Pressing either trigger pauses autodispensing until either trigger is pressed again, when autodispensing restarts. At the end of the last dispensed aliquot, press RESET to begin the next pickup. Note for 2x8- and 24-channel models On the 2x8-channel and 24-channel models, both rows of tips need to be touched-off. Normally, dispense and blow-out are performed automatically by EDP3-Plus as you press and release the trigger for dispensing. This may not provide enough time to touch-off the second row on double-row models, if you touch-off each row separately. You can delay the dispense / blowout sequence and obtain more time for touch-off by holding-in the dispense trigger for the dispense stroke, instead of pressing and releasing the trigger. Hold the trigger only long enough to touch-off both rows of tips, then release the trigger. You can also touch-off both rows of tips at the same time; touch off all tips together at the bottom of the wells. Practice both methods to develop your technique. Power requirements for E12-1200 E12-1200 has different power requirements than other RAININ electronic multichannel pipettes. It can be operated (a) with the battery, (b) with the battery as well as connected to the option- al wall power supply. However, the high forces in E12-1200 preclude operation with the wall power supply alone. Autodispensing set in MULTI mode Note: AUTO symbol flashes when paused and is steady when active FIGURE 5: E2X8-20 (L) AND E24-100 (R)

lines when establishing your own performance specification. Service If you experience any problems with your multichannel EDP3 pipette contact RAININ Technical Support at 800-543-4030. Volume Increment Accuracy Precision Model µL µL % µL (±) % µL (≤) E8-10, E12-10 1 0.01 2.5 0.025 1.2 0.012 (range 0.5-10 µL) 5 1.5 0.075 0.6 0.03 10 1.0 0.10 0.4 0.04 E8-20, E12-20 2 0.02 7.5 0.15 2.0 0.04 E2X8-20, E24-20 10 1.5 0.15 0.5 0.05 (range 2-20 µL) 20 1.0 0.20 0.3 0.06 E8-50, E12-50 5 0.05 3.5 0.18 1.5 0.075 (range 5-50 µL) 25 1.2 0.30 0.4 0.1 50 0.8 0.40 0.2 0.1 E2X8-100, 10 0.1 3.5 0.35 1.0 0.1 E24-100 50 0.8 0.4 0.24 0.12 (range 10-100 µL) 100 0.8 0.8 0.15 0.15 E8-200, E12-200 20 0.2 2.5 0.5 1.0 0.2 (range 20-200 µL) 100 0.8 0.8 0.25 0.25 200 0.8 1.6 0.15 0.3 E8-300, E12-300 30 0.2 2.5 0.75 1.0 0.3 (range 20-300 µL) 150 0.8 1.2 0.25 0.375 300 0.8 2.4 0.15 0.45 E8-1200, 100 1.0 3.6 3.6 0.60 0.60 E12-1200 600 0.8 4.8 0.20 1.20 (range 100-1200 µL) 1200 0.8 9.6 0.15 1.80 (Specifications subject to change without notice) Note: Unique tips for 1200 µL models The RT-L1200 and RT-L1200S tips are specifically designed for use with 1200 µL models in MULTIDISPENSE mode. The unique design of these tips prevents drop formation and eliminates the need for touch-off between dispenses in MULTIDISPENSE mode. The RT-L1200F aerosol-resistant tip allows a full 1200 µL to be aspirated under the filter. However, fluid dynamics dictate that touch-off may be required when multidispensing. all-g uide s.co m

Pipettes: 8-channel E8-10 8-channel, 0.5 to 10 µL E8-20 8-channel, 2 µL to 20 µL E8-50 8-channel, 5 µL to 50 µL E8-200 8-channel, 20 µL to 200 µL E8-300 8-channel, 20 µL to 300 µL E8-1200 8-channel, 100 µL to 1200 µL 12-channel E12-10 12-channel, 0.5 to 10 µL E12-20 12-channel, 2 µL to 20 µL E12-50 12-channel, 5 µL to 50 µL E12-200 12-channel, 20 µL to 200 µL E12-300 12-channel, 20 µL to 300 µL E12-1200 12-channel, 100 µL to 1200 µL 2x8 (16-channel) E2X8-20 16-channel in 2x8 configuration, 2 µL to 20 µL E2X8-10016-channel in 2x8 configuration, 10 µL to 100 µL 24-channel E24-20 24-channel in 2x12 configuration, 2 µL to 20 µL E24-100 24-channel in 2x12 configuration, 10 µL to 100 µL Note: LTS multichannel pipettes require LTS tips. Accessories: HU-M3 Package of 3 Magnetic Hang-Ups. Includes adhesive disk for attachment to a non-ferrous vertical surface HU-S3 Shelf clamp with 3 Hang-Ups attached CR-7 Free-standing Pipette Carousel, for up to 7 pipettes HU-A3 Adapter for Multichannels for HU-M3, HU-S3 & CR-7 6107-040 Battery 6107-249 Battery for E12-1200 Note: One of the following charging devices must be used to charge EDP3-Plus battery. E3-WPS Wall Power Supply. Recharges EDP-Plus in about an hour. Allows the pipette to be used while being charged. E3-RCS Rapid Charge Stand. Recharge up to three EDP3-Plus pipettes. Includes E3-WPS. Bench or wall mounting. To order pipettes or accessories, call 800-472-4646, or visit www.rainin.com on the web. RAININ is a registered trademark and EDP3 and LTS are trademarks of Rainin Instrument, LLC. EDP3 Pipettes are manufactured under U.S. Patents. 4,671,123, 4,905,526, 5,187,990, 5,614,153, 6,299,841 and 6,254,832. LTS LiteTouch Tip Ejection System is protected under U.S. patents 6,168,761 B1 and 6,171,553 B1. Other U.S. and national patents pending. Prices and specifications subject to change without notice.

7500 Edgewater Drive, Oakland, CA 94621-3027 a METTLER TOLEDO Company ©2000-2003, Rainin Instrument,LLC. All rights reserved. Prices and specifications are subject to change without notice. 9920-256 Rev J Limited Warranty See the enclosed Limited Warranty and Limitations of Liability Statement. Please complete and return the Warranty Registration Card on receipt of your pipette. RAININ LTS pipettes are calibrated with RAININ LTS tips. To assure excellent reproducibility and performance, use only RAININ LTS tips as recommended in this manual. Specified performance is guaranteed only when RAININ LTS tips are used. Contacting RAININ Technical Information: Phone: 800-543-4030 Fax: 781-938-1152 E-mail: [email protected] Pipette Service: Phone: 800-662-7027 Fax: 781-935-7631 E-mail: [email protected] Direct Order Line: Phone: 800-472-4646 Fax: 781-938-1152 E-mail: [email protected] Web: www.rainin.com From outside the U.S.: California Office: +1 (510) 564-1600 Massachusetts Office: +1 (781) 935-3050 RAININ Japan: Phone: (03) 5689-8311 Fax: (03) 5689-2670 Other International Offices and Distributors: See www.rainin-global.com E-mail: [email protected] (from outside the U.S.) Web: www.rainin-global.com (from outside the U.S.)