Jeio tech IST-3075 Operating Manual

Menual no. : XXXXXXXXXXX

carefully. Users should follow all of the Operational Guidelines contained in this Manual and take all necessary safety precautions while using this product. Failure to follow these guidelines could result in potentially irreparable bodily harm and/or property damage. Thank you for purchasing Jeio Tech’s products. We are doing best for customer’s satisfaction.

Jeio Tech Co, Ltd. is dedicated to providing world-best product quality and customer satisfaction. To ensure we maintain this commitment we have developed and implemented a total quality program, which conforms to the requirements according to DIN EN ISO 9001:2000 for the design, development, production, sales and servicing of biotechnology, environmental chemical engineering related products, and reliable measuring equipment for electric and electronics (ovens, incubators, constant temperature humidity chambers, constant temperature baths, refrigerating bath circulators, heat exchangers and shakers). Visit our Web site at www.jeiotech.com to view a copy of our certificate. Disclaimer Jeio Tech Co., Ltd. is committed to a continuing program of product development and improvement, and reserves the right to change information, such as specifications, appearance, and dimensions, described in this document without notice. Copyright No part of this manual may be reproduced or transmitted in any form or by any means, including photocopying, recording, or using information storage and retrieval systems, for any purpose other than the purchaser's own use, without the express written permission of Jeio Tech Co., Ltd. ©2014. All Rights Reserved. Jeio Tech Co., Ltd. Any other product names and services identified in this manual are trademarks or registered trademarks of their respective owners. No such use, or the use of any trade name, is intended to convey endorsement or other affiliation with Jeio Tech Co., Ltd

1.2 Symbols used in this Manual ................................................................................................................................１ 1.3 Exemption for responsibility .................................................................................................................................２ 1.4 Warning statement .....................................................................................................................................................２ 1.5 Caution statement ......................................................................................................................................................４ 2 Functional Description ....................................................................................................................................................７ 2.1 Introductions .................................................................................................................................................................７ 2.2 Features ........................................................................................................................................................................７ 2.2.1 High Performance ......................................................................................................................................７ 2.2.2 Advanced Convenience ...........................................................................................................................８ 2.2.3 Advanced Safety .........................................................................................................................................９ 2.3 Structure .................................................................................................................................................................. １０ 3 Installation ....................................................................................................................................................................... １４ 3.1 Unpacking and Checking .................................................................................................................................. １４ 3.2 Component ............................................................................................................................................................ １４ 3.3 Preparation before installation ....................................................................................................................... １５ 3.3.1 Space requirements ............................................................................................................................. １５ 3.3.2 Environmental setting .................................................................................................................................... １７ 3.4 Installation .............................................................................................................................................................. １８ 3.4.1 Caution for Installation ................................................................................................................................ １８ 3.5 Attaching Condensate Drain ........................................................................................................................... １９ 3.6 Attaching/Detaching External Refrigeration (IST-3075, IST-4075 Only) ....................................... １９ 3.7 Connection power ................................................................................................................................................. ２１ 4 Operation ......................................................................................................................................................................... ２２ 4.1 Controller .................................................................................................................................................................. ２２ 4.2 How to use the controller ................................................................................................................................ ２３ 4.2.1 Temperature control .............................................................................................................................. ２５ 4.2.2 Shaking Control ............................................................................................................................................... ２６ 4.2.3 Shaking Timer .................................................................................................................................................. ２７

4.3 General Settings .................................................................................................................................................. ３１ 4.4 System Settings ................................................................................................................................................... ３２ 4.4.1 Control Deviation Limit ................................................................................................................................ ３２ 4.4.2 External Start/Stop Key ................................................................................................................................ ３３ 4.4.3 Calibration .......................................................................................................................................................... ３３ 4.4.4 Auto Tuning ....................................................................................................................................................... ３６ 4.4.5 Operating Parameters .................................................................................................................................. ３６ 4.4.6 Reset ..................................................................................................................................................................... ３７ 4.5 Safety Tool ............................................................................................................................................................... ３７ 4.5.1 Over Temp. Limiter ......................................................................................................................................... ３７ 4.5.2 Glass Fuse .......................................................................................................................................................... ３８ 5 Maintenance ................................................................................................................................................................... ３９ 5.1 Inspection Period .................................................................................................................................................. ３９ 5.2 Cleaning .................................................................................................................................................................... ４０ 5.3 Air filter cleaning (IST-3075R/IST-4075R) ................................................................................................ ４１ 5.4 Fuse Replacement ............................................................................................................................................... ４２ 6 Troubleshooting ................................................................................................................................................................. ４４ 6.1 Stop during the operating ............................................................................................................................... ４４ 6.2 Another problem and solution .......................................................................................................................... ４５ 7 Accessories ...................................................................................................................................................................... ４７ 7.1 Mountable maximum quantity.......................................................................................................................... ４７ 7.1.1 Universal Platform + Flask Clamp .......................................................................................................... ４７ 7.1.2 Universal Platform + Plastic Flask Clamp............................................................................................ ４７ 7.1.3 Universal Platform + Funnel Clamp....................................................................................................... ４８ 7.1.4 Universal Platform + Microplate Holder ............................................................................................. ４８ 7.1.5 Universal Platform + Test Tube Rack .................................................................................................... ４８ 7.1.6 Spring Wire Rack + Flask ........................................................................................................................... ４９ 8.0 S/W ....................................................................................................................................................................................... ５０ 8.1 Monitoring Program installation ................................................................................................................... ５０ 8.2 Communication Protocol .................................................................................................................................. ５２ 8.2.1 Physical Layer ................................................................................................................................................... ５２ 8.2.2 Model System number................................................................................................................................. ５２ 8.2.3 Modbus Protocol Address Definition .................................................................................................... ５２ 8.2.4 Modbus Protocol Description ................................................................................................................... ５４ 9.0 Appendix ............................................................................................................................................................................ ５９ 9.1 Technical Specifications ..................................................................................................................................... ５９ 9.2 Circuit Diagrams.................................................................................................................................................... ６１

9.2.2 External refrigeration system (IST-3075/IST-4075) ........................................................................... ６１ 9.2.3 Internal refrigeration (IST-3075R/IST-4075R) ...................................................................................... ６１ 9.3 Disposing of products .......................................................................................................................................... ６２ 9.4 Warranty ................................................................................................................................................................... ６２ 9.4.1 Terms of Warranty Service .......................................................................................................................... ６２ 9.4.2 Warranty exception ......................................................................................................................................... ６３ 9.4.3 Service and technical advice ...................................................................................................................... ６３

This manual is intended for individuals requiring information about the use of product. Use this manual as a guide and reference for installing, operating, and maintaining your Jeio Tech product. The purpose is to assist you in applying efficient, proven techniques that enhance equipment productivity 1.2 Symbols used in this Manual (1) The alert marks are for safety operation and protect user and instrument from Damage. (2) Signal word panels are a method for calling attention to a safety messages or property damage messages and designate a degree or level of hazard seriousness. (3) Pay attention enough to the contents of alert marks. Signal word panels Uses Indicates a hazardous situation which, if not avoided, will result in death or serious injury Indicates a hazardous situation which, if not avoided, could result in death or serious injury.

not avoided, may result in minor or moderate injury. Indicates a property damage message. 1.3 Exemption for responsibility (1) The claim which is out of the quality guaranteed by the manufacturer is out of manufacturer’s responsibility. (2) The damage which is from unexpected fault or damage of user by Acts of God is out of Manufacturer’s responsibility 1.4 Warning statement Indicates a hazardous situation which, if not avoided, could result in death or serious injury. Please use the product in safety facility installing laboratory in case of accident. Installed the product on durable and flat surface. Please, make sure safety equipment with relevant provision before handling the sample

Do not use the machine near to places where explosion can be happened due to organic evaporating gases. Explosive materials: Acid, Esther, Nitro compound Inflammable materials: salt peroxides, inorganic peroxide, salt acids. Do not use the machine at places where moisture is high and flooding can be happened. Please check and connect properly -the voltage, phase and capacity of power supply on the ID plate before installation. Be sure to install a separate power wiring and use a dedicated power supply. Power supply must be properly grounded. Abnormal grounded connection causes serious damage. Grounded connection must not be on the water pipe and gas pipe. Put off the power plug if some sounds and burning smell, smokes are happened. And request the service Stop the product operation and request service. Do not assemble, repair, modify on your own.

cannot get after service by warranty regulation 1.5 Caution statement Indicates a hazardous situation which, if not avoided, may result in minor or moderate injury. Be sure to disconnect the power after turning off the power switch. This is the safety regulation for next user. Do not put heavy things on the power line. Do not put the machine on the line. It may take off the wire coating and causes the electric shock or fire. Do not touch it with wet hands and put the main plug correctly. It may cause the electric shock or injuries. Do not inject any liquid and inflammable things inside of product.

It causes abnormal operation or trouble. It may deteriorate the ability of the product and not obtain correct results. Do not install the stirrer neat machinery generating high frequency noise. Please avoid installed from high frequency- welding machine, sewing machine, and mass SCR controller Do not sprinkle insecticide or flammable spray on the product. Use smooth cloths. Cleaning with solvent can cause fire and deformity. Please power off while product cleaning. It may cause the electric shock or fire. Wear protective gloves. Wear eye protection. No water No corrosive Electrical shock. Flammable

2.1 Introductions The cultivation environment such as temperature, pH, oxygen concentration, nutrition supply have a significant impact on cell cultures of animals and plants or proliferation of microorganisms. This incubated shaker is to provide a suitable environment for the cultivation through controlling constant temperature and shaking speed. The main uses of this device are as below:  Cell culture  Extractions  Solubility studies  Hybridization  Plasmid purification  Protein expression 2.2 Features 2.2.1 High Performance (1) Precise and fast temperature / shaking speed control is available by microprocessor PID feedback control. PID feedback control ensures the same experimental environment conditions for the user. The device’s deviation of set temperature and set rpm are each under ± 0.1 ℃ (standard: 37 ℃), ± 1% (standard: set rpm) which are regarded as very

user. (2) Control range of temperature and stirring speed is wide. Temperature control up to 80 ℃ and stirring speed control up to 500rpm are available to provide users wider experimental conditions. (3) Optimizing the structural design of the product enables stable shaking even with heavy load. Low center of gravity minimizes noise and vibration. (4) The accurate temperature control is available on the three temperature points. At the three points, the user adjust the temperature as same as the external calibration sensor. 2.2.2 Advanced Convenience (1) Touch LCD built user-friendly interface. LCD provides intuitive operation status check and easy operation. (2) Timer and operation time check of shaking performance is available. Setting and checking the value is available up to 999 hours and 59 minutes. (3) Convenient sample monitoring without affecting inside environment thanks to the three side transparent acryl lid with bright LED lamps. (4) Sturdy and light-weight acryl lid for effortless opening and closing the door. (5) Not only the conventional RS-232 port but also USB port is provided so it is easy to control the unit with PC. (6) By providing an automatic power failure recovery, even if the product gets power back after a momentary power failure, the auto run function automatically runs the product. (7) This equipment is more convenient when mounting / desorption of the sample. When

platform is fixed to easily replace the sample. 2.2.3 Advanced Safety (1) Smooth shaking start and stop mechanism minimizes the opportunity of reagent leakage. (2) In the case of the over load, the unit adjust itself with controllable rpm. There is no move cased from the vibration of the unit. (3) There is an independent adjustable temperature limit device in addition to the main controller in this device. If temperature rises more than the specified value due to equipment error, the independent device protects the internal sample and equipment by blocking the power supply of the temperature-related equipment. (4) When shaking operation is not possible due to the obstacle interfering the system, over current protection device stops the operation.

You can monitor the inner samples without opening the door during operation. (2) Gas Spring Door can stay opened for the user’ convenience when sample replacement. (3) LED Lamp Long lasting less electricity consumption LED is installed to check the inside of the chamber. (4) Temperature Sensor It is Pt 100Ω sensor that temperature and resistance values are shown as the linear format and response speed is fast.

(6) Over Temperature Limiter Over temperature limiter is independently configured from the main controller. The inner temperature of the chamber exceeds more than the set temperature, the device cut off the power to the power switch for the secure condition. (Refer to ‘4.5 Safety Device’) (7) Shaking Table Shaking table is connected to a shaking system and Accessory such as Universal Platform can be equipped on shaking table. (8) Control Panel There are independent displays for each Temperature, RPM, Timer and there are and start/stop buttons and LED on/off buttons. (9) Computer Interface The unit can be controlled by PC through USB port and RS-232 port. In the case of connecting both of the port, the USB port will be priority. (10) Condensate Drain Barb In case of the liquid leakage caused by spilled reagent or cracked flask inside of the chamber, the liquid is designed to be drained out through the drain of the unit. The drain barb is made of chromium plated copper and it is easy to connect 6mm ID soft-walled tubing to the condensate drain. (Refer to ‘3.5 Attaching Condensate Drain’) (11) Power Switch and Fuse Power switch provide the main power to the device. Fuse protects the device from instantaneous overcurrent. In case of fuse replacement, check the rated power. (Refer to 5.4

There is a door limit switch between the door and the appliances mainframe’s upper parts. If the door is opened, Shaking, Blower and Heater operation automatically stops. After 3 minutes at this status, warning sound alarms users to close the door. If a user does not shut a door so that five minutes passes, and an alarm sound continuously rings. And it blocks off the power to be authorized with a power switch, and Off gets a power switch done and all blocks off 2 phase of the power supplied with to an each part of appliances, and configuration does the safe state that only a Ground part is connected. (13) External Refrigeration Port (IST-3075/IST-4075only) Even if the model such as IST-3075/4075 which has no refrigerator, It can lower the temperature in the chamber by receiving refrigerant from the external refrigerant device such as chiller. The In/out ports are located on the back and bottom side of the device. These brass push-to-connect fittings accept Ø8mm OD hard-wall tubing and provide access ports for external refrigeration. (Refer to ‘3.6 Connection to External Refrigeration System’) (14) Condenser Cover and Filter (IST-3075R/IST-4075R only) Attachable condenser cover and filter prevent dust to the inside system. (15) Foot Foot fix the device for stable operation.

(1) Inspect the shipping container carefully for any damage. (2) Remove the outer container. (3) Before use, inspect the product carefully for any damage that may have occurred during shipping. (4) Report any damage to your local Jeio Tech office or the distributor. 3.2 Component (1) After unpacking, check the components. (2) In the case of omission of components, contact to Jeiotech. Item Figure Quantity Description Main Body 1 Fuse 2 JEIOTECH SOFTWARE CD 1

3.3.1 Space requirements It is essential that the product to be situated in an area where there is sufficient space for the product. Below figures show the minimum space requirements needed to properly operate and maintain the product. Dimensions (mm) Models A B C D IST-3075(R) 440 785 510 860 IST-4075(R) 540 890 510 915

The unit can be operated properly under the following environmental conditions for a long time running without any problem. No direct sunlight on the product Ambient temperature: 5℃ ~ 40℃ (41℉~104℉) Relative humidity not to exceed 80% Altitude not to exceed 2000m (6,562 feet) Connect the product to earth grounded terminals only. • Please install on the sturdy surface laboratory which is set safety facility and make sure horizontal align correctly. • Do not use the Product near environments where flammable gas may leak. • Moving casters must be changed height adjustable foots so that the unit is not moving.

(1) The device is recommended to use under the room temperature and humidity (30°C, 80%RH) do not install it near the Heat devices like a Heater. (2) Have enough space for door opening. (refer to 3.3.1) (3) Please install it on the sturdy surface laboratory and do not throw down or gives a big shock. (4) Locate it 1.5m away from any light devices and 30 CM away from the wall. (5) Install it on the sturdy leveled surface to prevent abnormal turbulence and noise. 1.5m(5.0') 30.0cm (12.0”) 30.0cm (12.0”) WALL WALL

In case of the liquid leakage caused by spilled reagent or cracked flask inside of the chamber, the liquid is designed to be drained out through the drain of the unit. The drain barb is made of chromium plated copper and it is easy to connect 6mm ID soft-walled tubing to the condensate drain. 3.6 Attaching/Detaching External Refrigeration (IST-3075, IST-4075 Only) Many laboratories are built with central chilling systems. With this in mind Jeio Tech has developed the IST-3075/4075 so that it can be connected to external refrigeration systems. To connect the unit to an external refrigeration source use Ø8mm OD hard-walled tubing, such as nylon or polyethylene. Note: The ISS-3075/4075 controller does not have the ability to communicate with the external refrigeration source. Use the following procedure to connect/disconnect the tubing to the unit. Use the following procedure to connect/disconnect the tubing to the unit.

Hold the tubing firmly and push it tightly into the fitting. Run the tubing to the external refrigeration source. (2) Removing from fitting Grab the tubing tightly and push towards the incubated shaker as you firmly push the collet release button with two fingers. Continue to push the collect release button, and pull the tubing out of the fitting.

JEIOTECH’s Shaking Incubators use a single-phase current. Use a suitable plug as the picture bellow by Identification label. Voltage is the 10% of applied voltage. • Connect the power with checking the voltage, Phase, Capacity. • Use the ground power for the connection. Do no use the double cap or a current tap socket causing a damage on the cable and fire due to an overcurrent. 110/120VAC 220/230VAC

display is appeared. 1 Power ON indicator Power is supplied. 2 Temperature Control ON indicator Under the temperature controlling. 3 Shaking Control ON indicator Under the shaking controlling. In the case of the timer is set, it is off. 4 Main Display Main Display for the operationg 5 General Setting General setting. 6 System Setting Parameter setting is available. For change the parameter, acknowledge the manual. 7 Quick Start/Stop Touch button is the base. Start/Stop external button is installed on the controller for the user’s convenience. Function setting is available on the system setting. (Default: shaking start/stop) 8 LED Lamp On/Off the LED lamp.

Setting is available on the main display. 1 Set RPM Display the setting RPM. Touch the screen, set the RPM. 2 Set Temperature Display the setting temperature. Touch the screen, set the temperature. 3 Set Timer Display the shaking time. Touch the screen, set the timer. In the case of no timer is set, the operating time is displayed. (999hours 59minutes) 4 Actual Temperature Display the temperature of inside of chamber. 5 Actual RPM Display the present shaking speed. 6 Back From main display to home display. It is available when the unit is stop the operating.

supply is back, the previous operating is back. It is auto restart function. At the time Power-Ack button is appeared on the display. In the case of the shaking was operating, the operating time is appeared on the display with red words. Once touch the Power-Ack or change the setting value, The Power Ack display is disappeared. 8 Silent In the case of attention on the unit is required, the unit generates the alarm. Touch the silent, the unit stops the alarm. Once the problem is not eliminated, the unit generates alarm again. 9 Status Bar The unit stops the platform on the same position. This display indicates that the platform is on the right position. If the platform is not on the right position, the color will be changed as yellow. The unit provide USB port and RS-232 port. The display is on when the computer is connected. Report Only : / Slave Mode : Door is opened. LED lamp is on. Mute function. The setting is available by the General Setting. 10 Lock The lock function is to prevent the not proper setting change. Once touch the button, the display is changed same as . To unlock it, touch twice of the button. ->

(1) Start temperature control Touch the set temperature button on the main display. Setting display is appeared. Set the temperature with touch pad and touch the start button. The temperature control starts. SV and PV is displayed on the main display with green color. (2) Stop the temperature control Touch the set temperature button. Setting display is appeared. Touch the stop button. The temperature control stops. The temperature control starts. SV and PV is displayed on the main display with gray color. (3) Change the set temperature Touch the set temperature button. Setting display is appeared. Put the setting value on the touch pad and touch the enter button. SV is changed.  Temperature setting is one digit and one decimal point available. Touch the panel and put the numbers.  Move out from the setting mode with ESC button.  In the case of no touch for 10 seconds, the display changed to the main automatically.  Check the PV, RPM on the setting display is available. (right upper side)  The operating is maintained as previous during the setting.  Once the door is opened during the shaking operating, the heater and fan is stopped. Close the door before door error generating, the previous operating is back.

(1) Start shaking control Touch the Set RPM Button under the shaking stop status. Setting mode is appeared. Set the value on the display and touch the Start button. Shaking control starts. SV/PV rpm is changed to green color. (2) Stop the shaking control. Touch the Set RPM button under the shaking operating status. Setting mode is appeared. Touch the Stop button. Shaking stops. SV/PV rpm is changed to gray color. (3) Change the shaking speed Touch Set RPM button. Setting mode is appeared. Set the value on the display and touch the Enter button. The rpm is changed.  Move out from the setting mode with ESC button.  In the case of no touch for 10 seconds, the display changed to the main automatically.  Check the PV, RPM on the setting display is available. (right upper side)  The operating is maintained as previous during the setting.  Once the door is opened during the shaking operating, the heater and fan is stopped. Close the door before door error generating, the previous operating is back.  In the case of change the sample, stop the shaking and check whether the platform is completely stopped.

Timer is only available for shaking. Once the timer is set, the shaking stops after operating the set time and beep is generated. Setting time is on the display once the timer is set and ‘---:--‘ is on the display once the timer is not set. Remaining time is on the display once the timer is set and accumulated time is on the display once the timer is not set. (1) Timer ON Touch the Set timer on the main display. Timer OFF is on the display once the timer is set and Timer on is on the display once the timer is not set. Touch the Timer ON button and set the time on the display. Touch the Enter button. Check the set time on the main display. (2) Timer OFF Touch the Set timer on the main display. Timer OFF is on the display once the timer is set and Timer on is on the display once the timer is not set. Touch the Timer OFF button and touch the Enter. Time display on the main display is changed to “---:--“. (3) Change the setting time Touch the Set timer on the main display. On the Timer ON status, put the time on the display. Touch the Enter. Check the changed time on the main display.  Once the setting time is changed during the timer operating, original set time is changed. The accumulated time is applied to the new time setting and the remaining time is calculated and displayed. If you want whole new time setting, off the timer and set the timer again.

numbers on it.  Move out from the setting mode with ESC button.  In the case of no touch for 10 seconds, the display changed to the main automatically.  On the timer set display, you can check the present chamber temperature and rpm. (right upper side of the display)  Below 1 minutes is displayed as“mmm:ss”.  Timer is not counted during the door open. 4.2.4 Alarm and Stop by force The unit senses the problem on the unit and warn to the user. The warning is visual-audial effect. The alarm is divided into warning and fault. 4.2.4.1 Warnings Warning generates visual-audial alarm but the unit is operating. Once the warning is generated, Silent button is appeared on the main display. Once the silent button is touched, next 15 minutes there is no alarm. If the problem is on-going after 15 minutes, the alarm is generated again.

temperature is more than the range of the permission. The unit check it once the present value reached to setting value. Once the gap is over the permission range, the alarm is generated. 2 RPM Deviation The rpm difference between setting rpm and present rpm is more than the range of the permission. The unit check it once the present value reached to setting value. Once the gap is over the permission range, the alarm is generated. 3 Load Unbalance Once the shaking system is vibrated by the present operating, the system adjusts the shaking speed to the controllable stage. This is on the display. 4 Door Open Once the door is opened, the temperature and rpm are not operated. Once the door is opened, visual-audial alarm is generated. After 3 minutes of door opening, the alarm is effective. Once the door is closed, alarm is eliminated and the operating is back.

 Deviation Alarm is not effective once the present value reaches to the setting value. (set time, in permission range)  Deviation alarm is initialized under the below situation. - Set the setting value - Get back to the work after stopping the control. (Door open, Stop operating)  Once the Deviation initialized, the unit check the present value. If the temperature not reaches to the SV for 4 hours and the rpm not reaches to the SV for 10 minutes, Deviation check is started.  The range of permission and the period of Deviation is changeable on the System Setting.  Once Load Unbalance is generated and the setting rpm is changed, the changed rpm is on the display with red color. 1 Temperature Limit Digital temperature regulator is active. The temperature of inside of chamber is out of range. Stop the unit for unit and user’s safety. Check the range of the temperature regulator. 2 Load Unbalance Shaking system has severe vibrating so that the unit must be stopped. Unbalanced sample attaching, heavy sample attaching, un- flat ground are the causes. Shaking system is stopped. 3 Door Open Once the door is opened for 5 minutes, the unit is stopped and generates the alarm. 4 Sensor Fault Temperature sensor is faulty so that the operating is stopped. 5 Over Current Over current is generated so stop the unit. Main causes are over-load and obstacle inside of the system.

protection. Fault alarm are over-temp protection, Load Unbalance, Door Open, Sensor Fault, Over Current. 4.3 General Settings Set the general operating. Touch General Setting button and below setting menu is appeared. Touch the ‘<’ or ‘>’ and explore the menu. 1 Shaking Direction Set the shaking direction • CW(clock wise), CCW(counter clock wise) 2 Temperature Unit C°/F° 3 Lock Use the Lock or not 4 System Sound Mute for sound or not 5 LCD Brightness Adjust the brightness of LCD 6 LCD Auto Dim LCD Dim function (No input, LCD dim) 7 Dim Brightness LCD Dim, and the brightness of the dim 8 Dim Time Out Waiting time for LCD Dim mode operating 9 Light Brightness Select the brightness of LED 10 Light Auto Door opened, auto LED light on 11 Auto Restart Auto restart function. The unit is back to the operating, before the power out.(Time information is not back) 12 USB (RS232) USB and RS-232 port is available for the PC. Select the mode when PC is connected. Report Only : PC received the information from the unit. Slave Mode : PC control the unit.

system. Touch the System setting button on the home display, below clauses are arranged. 1 Control Deviation Limit Set the deviation limit range. Temperature setting and rpm setting are available. 2 External Start/Stop Key Start/Stop membrane button is located on the control panel. Set about this key 3 Calibration Calibration on the temperature sensor and rpm to the user’s reference. Calibration on the LCD touch coordinate is available. 4 Auto Tuning Temperature PID parameter update. Optimum control on the main operating temperature is available. 5 Operating Parameters Set the operating and error check 6 Reset Set the parameter as initial factory value. 4.4.1 Control Deviation Limit Set the deviation limit between setting value and actual value of the temperature and rpm. The time of out of deviation limit range can be set either. 1 Temperature Deviation Limit Set the deviation limit between setting value and actual value. Set on the key pad. 2 Temperature Deviation Alarm Delay Once the deviation is out of range for alarm delay period, the alarm is generated. Once the actual temp reached to the set temp, the alarm is disappeared after alarm delay period check. 3 RPM Deviation Limit Set the deviation limit between setting value and actual value. Set on the key pad. 4 RPM Deviation Alarm Delay Once the deviation is out of range for alarm delay period, the alarm is generated. Once the actual rpm reached to the set rpm, the alarm is disappeared after alarm delay period check.

External Start/Stop button is on the control penal as membrane button. It generates start and stop. Set the applying range of it. 1 Only Shaker The button only works for shaker’s start/stop. 2 Only Incubator The button only works for incubator’s start/stop. 3 Shaker Incubator The button only works for shaker and incubator’s start/stop.  Shaker and incubator are both available for the external membrane button. Once one of the shaker/incubator is on the generating and touch the button, the stopped part is operated. Touch the button again, both of mode are stopped. 4.4.3 Calibration The temperature sensor and rpm reached to the user’s reference measure value, accurate experiment circumstance can be made. Calibration for coordinate is available. 4.4.3.1 Temperature Calibration Calibration for temperature sensor of main controller. The unit provides three points calibration. Each point of three shows the uncalibrated and calibrated status. Touch the point you want to change. Touch the one point of Calibration 1,2,3. Put the external sensor on the chamber and close the door.

Wait until the actual temp reaches to the setting temp. Once the actual temp reaches to the setting temp, touch the calibrated temp and put the temperature from the external sensor. Touch the confirm for the saving. The unit stops the operating. The unit read the temp as the external sensor’s value.

stopped.  The calibration is operating on various points so it limits the calibrating that is our of the logic.  The result of the calibration is on the uncalibrated and calibrated. Uncalibrated means the value from the unit and the calibrated means the temp that calibrated. The calibrated value is from the external sensor.  The calibration setting is available to be initialized on the System Setting Reset. 4.4.3.2 Shaking Speed Calibration The user calibrates the unit’s rpm with external sensor. The unit is available for one point rpm calibration. The procedure is similar with temperature calibration. The difference is that the shaking is not stopped during the door opening because the door need to be opened to check the shaking with external sensor. Touch the set rpm. Put the desired rpm and start the nut. Wait unit the actual rpm reached to the set rpm. Touch the calibrated rpm and put the value from the external sensor. Touch the confirm button to save it. The unit read the calibrated value from the next operating.  Be careful that the external sensor never touch the platform. Once the external sensor is removed from the platform during the operating, it will be dangerous.  The calibration setting is available to be initialized on the System Setting Reset.

Touch penal coordinate could be in the problem for the reason of the older unit and the problem in the unit. The unit has a touch screen coordinate function. Touch the “+” on the screen three time s. And repeat it two times. 4.4.4 Auto Tuning Update the parameter of PID temperature control. Optimum temperature for the main using temperature is available. The unit is set to use the temperature on proper range. Once the temperature is not actual on the main points during the actual use on the field, operating auto tuning will tune the temperature parameter and make the unit operates the temperature on the actual temperature. Touch the Set Temp. and put the desired value. Touch the START button and start the parameter tuning. Touch the stop button during the tuning and stop the tuning. Once the tuning is complicated, touch the ok button and save it. 4.4.5 Operating Parameters Set the parameters for the other operating. 1 Position Sensor Check the platform stop on the right position. 2 Stall Check Check the belt when the platform is not working properly. 3 Defrost Use Defrost is not for the IST model. Off the defrost use. 4 Load Unbalance Once the vibration is generated, the shaking speed is adjustable. 5 Threshold Load Unbalance check the vibration on the unit and adjust the rpm automatically. The vibration is on the range of not controlling, the automatic adjusting is operated.

1 Temperature Calibration Initialize the calibration value. 2 RPM Calibration Initialize the calibration value. 3 Auto Tuning Initialize the calibration value. 4 Touch Screen Initialize the calibration value. 5 Operating Parameters Initialize the calibration value. 6 Factory Default Initialize the calibration value of all parts.  Auto tuning parameter can be initialized to the factory value. 4.5 Safety Tool 4.5.1 Over Temp. Limiter This model has the over temp limit and temp sensor in the main controller. Over temp limit is located on the right down side of the unit. Set the over temp limit over 15% than normal use temperature by a flat head screwdriver. In case of that the temperature in the chamber over set temp, the operation discontinues.

In the case of the over current generating, the fuse is cut and the power is down.

Classification Inspection period Daily Weekly Monthly Quarterly Yearly General Power Connection between machine and power ● Power code ● Exterior cleanliness ● Drain connection ● Outside refrigeration system connection (IST-3075/IST-4075) ● Incubator Set temperature and actual temperature difference ● Auto Tuning ● Temperature setting Temperature correction ● Actual temp. / Display temp. correction ● Air filter clean (IST-3075R/IST-4075R) ● PC Sync operation ● Controller and timer operation ● System Connection between platform and accessories ● Connection between platform and shaking table ● Actual rpm/ Display rpm correction ● Malfunction of shaking system ●

Regarding the un-removable point, clean the polluted area only by towel with alcohol solvent (methanol, ethanol) which has low boiling point. (2) Do not use Acid solution, sharp one, soapy water, detergent and hot water. It makes the machine discolored. Rubber and plastic part can be change of shape and color. Especially, do not use volatile matter. In case of neutral detergent, clean it with the soft fabric and dry well. (3) Do not put the water to exterior of the machine when you clean the surrounding (Especially, socket and controller part, it can make short circuit problem.) (4) After discussion the proper cleaning method to avoid any damage for the machine with Jeiotech, if you want to clean the machine or remove the polluted area with not mentioned cleaning method before the clean. (5) The inside electric part of the machine should be handled by Jeiotech or person who is delegated by Jeiotech. (6) If the parts is required to replace, please use genuine parts only. (7) Technical maintenance is not offered in case of abnormal trouble beyond the normal limit. • Do not soak the machine into water and spray the water • Do not clean whole body with chlorine bleaching, detergent with chlorine, an abrasive, ammonia, steel sponge and alcohol solvent (methanol, ethanol) which has low boiling point.

Step 1: Put power switch and turn the machine off. Step 2: Loosen the bolt that located in edge of condenser cover Step 3: Disassemble condenser cover Step 4: Disassemble air filter which is combined with condenser cover. Step 5: Vacuum or clean the air filter with the water. Step 6: Vacuum or high pressure air clean to the condenser in the machine. It makes better refrigeration effect. Step 7: Assemble air filter in reverse order of Step 1~Step4 • When air filter clean, please be careful to avoid fold the condenser pin • It is possible to decrease refrigeration effect.

Replace the fuse which is located in next to power button (Refer to 2.3.(11)) if no operation of short circuit breaker and No power. There is extra fuse 2pcs in socket case inside. If you need additional fuse, ask it to sales team or seller. Model Voltage Current consumption(A) Fuse (A) Fust Cat. No. IST-3075 120VAC, 60Hz 7.5 10 00CDE0005543 230VAC, 50/60Hz 4 8 00CDE0005544 IST-4075 120VAC, 60Hz 10.1 12 00CDE0005542 230VAC, 50/60Hz 5.5 8 00CDE0005544 IST-3075R 120VAC, 60Hz 7.5 10 00CDE0005543 230VAC, 50/60Hz 4 8 00CDE0005544 IST-4075R 120VAC, 60Hz 10.1 12 00CDE0005542 230VAC, 50/60Hz 5.5 8 00CDE0005544 Fuse is located in next to power switch. (Side of machine.) Please replace the fuse according the below picture.

Before replace the fuse, turn the machine off and check the power connection again. If the power is connected, serious injury or death can be occurred. Step 1: Press power switch and Power off Step 2: Connect (-) screw driver to fuse holder Spin the fuse holder counterclock wise 50 ˚ then fuse holder is disassembled. Step 3: Compare the fuse specification with the above table and replace it if there is no problem. Step 4: Complete the replacement in reverse order of Step 2 퓨즈 교체 반시계방향으로 50도 회전 드라이버 삽입

Display Message Description Solution Temperature Limit Analog over temperature regulator is active. Check the set on the analog over temperature regulator and the operating. Restart after temperature is inside of the range. Load Unbalance Vibration is generating and the normal shaking is not available. To prevent the shaking system, stop the shaking. Check the unbalanced sample setting, overload, un-flat ground. Door Open Door is opened over 5 minutes during the operating. Close the door and touch the start button and restart the unit. Sensor Fault Temperature sensor is faulty so the operating is stopped. Contact the manufacturer or sales point. Over Current Over current is generated so the operating is stopped. Check the overload, obstacle in the shaking system.

Trouble Probable Cause & Solution The equipment is not on ① Check the power plug connection to the socket ② Check if the circuit breaker is operated ③ When the fuse is short circuited, replace new one as enclosed. ④ Check the power failure ⑤ If the problem is not fixed with the above method, please request A/S Power on but controller does not operated ① Check the power plug connection to the socket ② Check the panel board circuit breaker and reset it then turn the machine on ③ Request A/S if Display board or Main PCB has problem ① Request A/S if Power switch has problem. NO The temperature of incubator rise to setting value ① Check the Temperature Control ON indicator is ON ② Check outdoor temperature is too high or low and maintain the right room temperature. ③ Request A/S after check the difference between actual temperature and display temperature. ④ Check the door switch irregularity ⑤ Request A/S if the heater is out of order ⑥ Request A/S if the freezer is out of order. ⑦ Check air filter condition and clean it according to 5.3 ⑧ Auto Tuning if the temperature is unstable according to 4.4.4. ⑨ If the problem is not fixed with the above method, please request A/S.

during operation, check the horizontality. If you need, please adjust horizontality according 3.4.2 ② If there is extremely intense vibration of the machine and shaking speed is slow down, check the sample location and relocate the samples harmoniously ③ In case of overloading, control the sample weight according to 9.1(Max load per speed). ④ Check door switch if shaking is not operated after shaking start button press. ⑤ Check Timer close status if shaking is not operated after shaking start button press, cancel timer set-up. ⑥ If the problem is not fixed with the above method, please request A/S. Noise ① Check fixed condition of platform or sample. ② Check the weight of sample is shifted to one side, relocate the samples evenly. ③ If the problem is not fixed with the above method, please request A/S.

7.1.1 Universal Platform + Flask Clamp Model Flask Clamp IST-3075 series IST-4075 series 50ml 25 49 100ml 20 39 250/300ml 9 18 500ml 9 13 1,000ml 5 9 2,000ml 3 5 2,800ml 1 2 7.1.2 Universal Platform + Plastic Flask Clamp Model Flask Clamp IST-3075 series IST-4075 series 50ml 25 49 100/125ml 14 25 200ml 9 16 250ml 9 16 300ml 9 16 500ml 6 9 1,000ml 4 7 2,000ml 2 4

Model Funnel Clamp IST-3075 series IST-4075 series 250ml 4 6 500ml 2 4 1,000ml - 3 2,000ml - - 7.1.4 Universal Platform + Microplate Holder Model Type IST-3075 series IST-4075 series Single 7 13 Tower 5 8 Flat A(large) - 2 Flat B(small) 2 2 7.1.5 Universal Platform + Test Tube Rack Model Type IST-3075 series IST-4075 series 0° 2 4 15° 2 4 30° 1 3 45° 1 2 60° 1 1

Platform Flask IST-3075 series IST-4075 series 50ml 16 25 100/125ml 9 16 250/300ml 4 9 500ml 4 5 1,000ml 2 4 2,000ml 1 2 2,800ml 1 2 ※ About the right position for mounting maximum quantity of Accessories, please visit Jeio Tech website or contact our office and distributor.

(1) Put the installation CD on the CD-ROM drive then the program is operated automatically. Click the “Next” button go to the License Agreement. (2) Check the details and click the “I agree”. (3) Click the “Install” and install the program.

on the background. Check the Run JeioTechSoft 2.0.0.5 or double click the icon then the program starts.

The unit communicates with PC through RS-232C port. Software save the duplex communication, operating status, date. If you want to change on software, refer to below reference. Communicatioin Reference : http://www.modbus.org/docs/Modbus_Application_Protocol_V1_1b3.pdf 8.2.1 Physical Layer - Communication port : RS-232C /USB 8.2.2 Model System number ITEM System Number System Model Number Incubated Shaker 1611H Shaking incubator IST-3075 1612H Shaking incubator IST-3075R 1613H Shaking incubator IST-4075 1614H Shaking incubator IST-4075R 8.2.3 Modbus Protocol Address Definition modbus function code address command data description data length word byte W/S 1 Beep 0x0001 BEEP_SYS_BOOT (Communication Test) 1 2 0x0002 BEEP_KEY (Communication Test) 1 2 R/I 2 MOD_SYS_NA ME x Model name 1 2 R/I 3 MOD_SYS_VER x Firmware Version 1 2 R/I 4 MOD_SYS_PAR AM x system parameter 20 40 W/S, R/I 25 MOD_SYS_SOU ND 0/1 system sound 1 2 W/S, R/I 26 MOD_SYS_LAM P 1 ~ 3 lamp bright 1 2

1 2 W/S, R/I 28 MOD_AUTO_R ESTART 0/1 system auto restart 1 2 W/M, R/I 29 MOD_TEMP_DE VI temp devi min ~ temp devi max temperature deviation 4 8 W/S, R/I 33 MOD_TEMP_DE VI_ALARM 0 ~ 3599 temp devi alarm time 1 2 W/S, R/I 34 MOD_RPM_DE VI rpm devi min ~ rpm devi max rpm deviation 1 2 W/S, R/I 35 MOD_RPM_DE VI_ALARM 0 ~ 3599 rpm devi alarm time 1 2 W/S, R/I 36 MOD_EXT_KEY 0 ~ 2 external key operation 1 2 W/M, R/I 37 MOD_SET_TEM P low temp lmt ~ high temp lmt incubator set temp 4 8 W/S, R/I 41 MOD_SET_RPM rpm min ~ rpm max shaker set rpm 1 2 W/M, R/I 42 MOD_SHAKER_ TIME 60L ~ 3599940L shaker set time 2 4 W/S 44 MOD_SHAKER_ TIME_ONOFF 0 ~ 1 shaker timer set 1 2 W/S 45 MOD_INCUBAT OR_RUN_STOP 0 ~ 1 incubator operation set 1 2 W/S 46 MOD_SHAKER_ RUN_STOP 0 ~ 1 shaker operation set 1 2 R/I 47 MOD_SYS_REP ORT X system report 18 36 W/S 65 MOD_WARN_A CK 1 system warning ack 1 2 W/S 66 MOD_SILENT_A CK 1 system silent ack 1 2 W/S 67 MOD_AUTO_A CK 1 auto run ack 1 2

Command Description Beep Beep generating Modbus function code 0 Write data 1 System boot beep W.S Data 2 System key beep MOD_SYS_NAME Return the unit name Refer to System number Modbus function code 0 Return data 0 Upper Lower R.I address Model num MOD_SYS_VER Return the firmware version Modbus function code 0 Return data 0 Upper Lower R.I address version MOD_SYS_VER Return the system parameter Modbus function code 0 Return data 0 ~ 3 4 ~ 7 8 ~ 11 12 ~ 15 high temp lmt low temp lmt temp_d evi_ma x temp_d evi_min R.I address 16 17 18 19 max rpm min rpm rpm_de vi_max rpm_de vi_min MOD_SYS_SOUND Set the system sound modbus function code 0 return data 0 R.I address upper lower sound flag modbus function code 0 r/w data 0 sound off W.S data 1 sound on MOD_SYS_LAMP Set the lamp brightness modbus function code 0 return data 0 R.I address upper lower lamp bright modbus function code 0 r/w data 1 ~ 3 W.S data 1 : Min, 3 : Max

setting modbus function code 0 return data 0 R.I addres s uppe r lower lamp auto flag modbus function code 0 r/w data 0 lamp auto off W.S data 1 lamp auto on MOD_AUTO_RESTAR T Auto run setting modbus function code 0 return data 0 R.I addres s uppe r lower auto run flag modbus function code 0 r/w data 0 auto run off W.S data 1 auto run on MOD_TEMP_DEVI temperature deviation modbus function code 0 return data 0 ~ 3 R.I addres s temp devi modbus function code 0~3 value temp devi min ~ max W.M value MOD_TEMP_DEVI_ALAR M temp devi alarm time modbus function code 0 return data 0 R.I addres s temp devi alarm modbus function code 0 value 0 ~ 3599(sec) W.S value MOD_RPM_DEVI rpm deviation modbus function 0 return 0

max W.S value MOD_RPM_DEVI_ALAR M rpm devi alarm time modbus function code 0 return data 0 R.I addres s rpm devi alarm modbus function code 0 value 0 ~ 3599(sec) W.S value MOD_EXT_KEY external key operation modbus function code 0 return data 0 R.I address ext key value modbus function code 0 value 0 1 2 W.S value shaker incubator both MOD_SET_TEMP incubator set temp modbus function code 0 return data 0 ~ 3 R.I addres s set temp modbus function code 0 ~ 3 value high temp ~ low temp W.M value MOD_SET_RPM shaker set rpm modbus function code 0 return data 0 R.I addres s set rpm modbus function code 0 value rpm min ~ max W.S value

modbus function code 0 return data 0 ~ 1 R.I addres s set time modbus function code 0 ~ 1 value 0 ~ 3599940L(min) W.M value MOD_SHAKER_TIME_ONO FF shaker timer on/off set modbus function code 0 data 0 : shaker timer off W.S data 1 : shaker timer on MOD_INCUBATOR_RUN_S TOP incubator operation set modbus function code 0 data 0 : incubator stop W.S data 1 : incubator start MOD_SHAKER_RUN_STOP incubator operation set modbus function code 0 data 0 : shaker stop W.S data 1 : shaker start MOD_SYS_RE PORT system report modbus function code 0 return data 0 ~ 3 4 ~ 7 8 9 R.I addre ss set temp act temp set rpm act rpm 10 ~11 12 ~ 13 14 ~ 15 16 ~ 17 set time remai n time syst em indi cate dum my system indicate 4byt e MSB 30 29 28 27 26 25 24 dumm y dumm y dum my dum my usb_m ode temp delay auto tune sk over cur 0 : report Igonor e

modbus function code 0 data 1: message clear W.S data 0: operation none MOD_SILENT_ACK Mute modbus function code 0 data 1: beep stop W.S data 0: operation none MOD_AUTO_ACK auto run ack modbus function code 0 data 1: auto run clear W.S data 0: operation none

MODEL IST-3075 IST-3075R IST-4075 IST-4075R Temperature Range Amb.+5~80℃ Amb.-15 (Min. +15)~80℃ Amb.+5~80℃ Amb.-15(Min. +15)~80℃ Accuracy ±0.1℃ in flask at 37℃ Uniformity ±0.5℃ in flask at 37℃ Refrigerator (Hp) - 1/6Hp - 1/6Hp Sensor Type Pt 100Ω Shaking Motion Orbital Amplitude Size (mm, dia.) 19.1 Speed Range (RPM) 10 to 500 Accuracy ±1% of set speed (>100rpm) / ±1 (<100rpm) Timer 1 min. to 999 hr 59 min. Max. Load (kg) 10 at 500 rpm 15 at 400 rpm 14 at 500 rpm 21 at 400 rpm Material Internal Stainless Steel External Epoxy powder coated steel Platform Anodized aluminum plate Insulation Polyethylene Lid PET Heater Incoloy, Fin Type (400W X 2EA) Safety Device CLS(Custom Logical Safe)-control system Electronic temperature limiter, Hydraulic over-temperature limiter Temperature deviation alarm (High/Low) Communication Interface USB, RS232 Dimension Volume (L) 53 83 Platform (W×D) 350×350 450×450

540 x 890×510 Net Weight (Kg) 65 73 81 90 Electric requirements(230V) 50/60Hz, 4A 50/60Hz, 5.5A 50/60Hz, 4A 50/60Hz, 5.5A Cat. No. Electric requirements(110V) 60Hz , 7.5A 60Hz ,10.1A 60Hz , 7.5A 60Hz ,10.1A Cat. No.

9.2.1 IST circuit diagram 9.2.2 External refrigeration system (IST-3075/IST-4075) 9.2.3 Internal refrigeration (IST-3075R/IST-4075R)

Before you dispose product or the components 1. The equipment should be cleaned and decontaminated to protect workers servicing the equipment, the environment or the public purchasing surplus equipment because the incubated shaker can potentially be contaminated with biological material, chemicals or radioisotopes. Check with your institution or laboratory for individual policies and procedures for disposal of laboratory equipment. 2. Please contact your local governing body for regulations regarding disposal of electrical, electronic, metal (brass, aluminum, steel and stainless steel), refrigeration and rubber components. Jeio Tech recommends the user find a local scavenger or laboratory equipment recycler to properly dispose of the unit and its components. 9.4 Warranty 9.4.1 Terms of Warranty Service (1) Customer can get free warranty service for 2 years limited warranty from the date of shipping date when the machine is broken while operating. (2) When you ask for repairing, please check the below details first. • Date of purchase • Customer name / address / Phone number / E-mail • Fault status 7

Customer can’t get free warranty service in case of as below. • If the product is broken due to the user’s fault. • If the product is broken due to improper operation or storage. • If the product is broken due to improper modify or repairing. • If the product is broken due to overuse of voltage or earthshock. • If the product is broken without taking care of the “Notice” alerted on the manual. 9.4.3 Service and technical advice We, Jeiotech Co., Ltd. are doing best to give best support based on customer service system. When we get the symptoms, fault states, contact number by customer, we offer after sales service. International Sales Head Office (Korea) #1005, Byucksan Digital Valley 6-Cha, 481-4 Gasan-Dong, Geumcheon-Gu, Seoul 153-704, Korea Tel: +82 2 2627 3816 E-mail: [email protected]

19 Alexander Road, Unit #7. Billerica, MA 01821-5094, U.S.A. Tel: +1 781 376 0700 E-mail: [email protected] FAX: +1 781 376 0704 Europe (U.K. Branch) Unit 3, Tower Industrial Park, Chalgrove, Oxfordshire, OX44 7XZ, United Kingdom Tel: +44 1865 400321 E-mail: [email protected] FAX: +44 1865 400736 China (Shanghai Branch) B-705, Jingting Bldg. No.1000 Hongquan Rd, Minhang district, Shanghai, P.R.CHINA 201103 Tel: +86-21-5108-9161, 5414-8389 Fax: +86-21-5168-5414 E-mail: [email protected] South East Asia (Malaysia Branch) No 57-59, Jalan Adenium 2G/6, Pusat Perniagaan Adenium, 48300 Bandar Bukit Beruntung, Selangor Darul Ehsan , Malaysia Tel: +60 3 6021 6880 E-mail: [email protected] FAX: +60 3 6021 7880  Product is subject to change for improving quality and performance without an advance notice.  The copyright of this manual belongs to Jeiotech.