BMS D1 Series Instructions Manual

Microscope INSRUCTIONS This manual expatiates the using method, troubleshooting, and maintenance about the microscope particularly. Please read it before using carefully. The manufacturer is entitled to amend or improve the instrument. The quality for this microscope is according to GB/T2985-1999 biological microscope standard.

Before use 1. Nomenclature ……………………………………………………………………..……….1 2. Assemblage ……………………………………………………………………………….3 2-1. Assemble scheme …………………………………………………………………….....3 2-2. Assemble steps ……………………………………………………………………….....4 3. Operation………………………………………………………………………… ….……..6 3-1. Illumination …………………………………………………………………………..6 3-2. Set slides ………………………………………………………………………………..6 3-3. Focusing ………………………………………………………………………………..6 3-4. Adjust tightness of focusing………………………………………………………….....7 3-5. Adjust diopter …………………………………………………………………………..7 3-6. Adjust interpupillary distance ………… …………………………… …………………8 3-7. Adjust center of condenser ………………… ……………………………………….…8 3-8. View field diaphragm … ………………………………………………… ……………9 3-9. Aperture diaphragm………… ………………………………… ………………………9 3-10. Use objective with Oil …………………… ……………………… …………………10 3-11. Use filter ………………… ……………………………………………… ………….10 3-12. Replace fuse………………………………… ……………… ………………………10 3-13. Select light path ……………………………………………………………..……......11 3-14. Set video device ………… …………………………………………………..…….…11 3-15. Set photo adapter ………… …… …… ………………………………………..….…11 4. General specification …………………………………………………………….….… ..12 4-1. D1 series biological microscope specification …………………………………….….12 4-2. Objective parameters ……………………………………………………………….…12 5. Troubleshooting … ……………………………………………………………….….… ..13

1. Notice ⑴ As the microscope is a precision instrument, a always operate it with care, avoiding physical shake during the operation. ⑵ Do not let the microscope emerge in the sun directly, either not in the high temperature, damp, dusty or acute shake place. Make sure the worktable is horizontal. ⑶ When moving the microscope, you should use both hands to hold ① and ② as shown on figure, and lay it down carefully. (Figure 1) ★ If hold the focusing knob, eyepiece tube or stage during moving, you will damage it. ⑷During working, the light condenser will become hot. Please make sure enough space around ③ for cooling.(Figure 2) ⑸Connect the microscope to earth avoid lightning strike. ⑹ Make sure the power switch ④ is on “0” (off) before replacing the bulb or fuse and wait until the lamp cools down. (Figure 3 ★ Bulb: 6V/20W halogen bulb（Philips 7388） ⑺ It can adapt wide voltage 90~240V. There is no need for transformer. Make sure the voltage is in this range. ⑻ Use the special plug supplied by our company. Figure 2 Figure 3

tissue moistened with a little of 3:7 mixture of alcohol and ether or dimethy lbenzene. ★ Alcohol and ether is flammable. Don’t place these chemical near to fire or fire source. For example, when turning on or turning off the electric device, you should use these chemical in a ventilated place. ⑵ Don’t use organic solution to wipe the surfaces of the other components. Please use the neutral detergent. ⑶ If the microscope damped by the liquid, you should cut off the power immediately and wipe it dry. ⑷ Never disassemble or service the microscope yourself. It will influence its function or damage it. ⑸ After using, cover the microscope with a dust cover. 3. Safety Sign Sign Signification Read manual before using. Faulty operation would lead to person hurt or instrument trouble. | Power swatch ON O Power swatch OFF

BMS D1 binocular 1 Eyepiece Nosepiece Objective Stage Condenser for Normal illumination Light condenser for Normal illumination Focusing arm Fine adjusting knob Coarse adjusting knob Arm Sliding binocular head

2 Tightness adjusting knob Gemel trinocular head Light condenser for Kohler illumination Condenser for Kohler illumination Power switch Lock-screw Light adjusting knob

2-1 Assemble scheme: The below numbers denote the assembling older. ★ Before assembling, make sure there is no dust or dirt. Assemble carefully and do not scrap any part or touch the glass face. 3 Eyepiece Objective Condenser Plug halogen bulb

Set condenser for normal illumination 1.Turn the coarse knob with the direction showed to make the stage to highest position. (Figure 4) 2. Turn the condenser knob with the direction showed to make the condenser holder down to suitable position. 3. Loose the lock screw for condenser thoroughly. 4. Slide the condenser into holder with the direction showed till it is tied with the holder. And turn the condenser to make the knob direct to front. 5. Tight the lock screw  and use knob  moving the condenser up to highest position. Set condenser for Kohler illumination 1.Turn the coarse knob with the direction showed to make the stage to highest position. (Figure 5) 2. Turn the condenser knob with the direction showed to make the condenser holder down to suitable position. 3. Loose the lock screw for condenser thoroughly. 4. Put the screw behind condenser matching with the slot on condenser holder and slide it into thoroughly. 5. Tight the lock screw and use knob moving the condenser up to highest position. 2-2-2 Assemble objectives （Figure 6） 1. Turn the coarse knob ① with the direction showed to make the stage to suitable position. (Figure 6) 2. Assemble the objectives from low to high magnification clockwise.  Please use objective with low magnification (such as 4X or 10X) to find the image.  Please make sure correct position with a sound during turning the nosepiece. 4 图3 图14 135图4 图4 图4 Figure 5 Figure 4 Figure 6

◎ Please use assigned Philips 7388 halogen bulb 6V20W.  In order to use the bulb longer or prevent blowout, please do not touch it barehanded. Please swap it with soft cloth if you leave fingerprint on the bulb. 1. Loose the lock screw ① and turn the bulb holder ② clockwise. 2. Use gauze or others to take the bulb  and set it into bulb holder ④.  Please set the bulb softly, over press will damage it.  Use or replace bulb During operation or just after using, the bulb and bulb holder will be very hot. Please turn the switch to “0” and take off the plug. After the bulb and bulb holder cool-down, you can replace the bulb. This microscope is also suitable for LED illumination, the operation for LED is similar to halogen bulb. 2-2-4 Set eyepiece （Figure 8） 1. Take off the cap on eyepiece cap ①. 2. Slide the eyepiece ② into eyepiece tube throughly. 2-2-5 Connect plug （Figure 9）  Do not press the plug too much. Be aware that bend or enlacement will damage it. 1. Before connect the plug, please turn the switch to “OFF”. 2. Slide the plug ① into the socket ② on microscope and make sure good contact. (Figure 9) 3. Connect the other side of the plug to power supply and make sure good contact.  Please always use the plug supplied by manufacturer. If damage or lost, please use same specification one. ★ It adopt wildly power supply 90-240V. ★ Please connect the power supply correctly, be sure the instruments to be connected with earth. Figure 8 Figure 9 Figure 7

3-1 illumination （Figure 10） 1. Set the power switch ① at “ON” after connect power suppler. 2. Use knob ② to adjust the brightness more comfortable. Clockwise for brightness and counter-clockwise for darkness. (Figure 10)  Under low power, the bulb will have long life. 3-2 Set slides （Figure 11） 1. Push the clamp ①. 2. Put the slides ② on specimen holder and make sure the frontispiece up towards. Then loose clamp ① to tight the slides. (Figure 11) 3. Adjust the knob  to move the slides to center (according to center of objective). 3-3 Focusing （Figure 12、14、15） 1. Let objective 4X move into light path. 2. Turn one dioptor ring  to make it in line with reticle  on eyepiece tube. At this time, the position is “0”. (Figure 14) 3. Turn coarse knob  to find the image. (Figure 12) 4. Adjust anther dioptor to make the image clear. (Figure 14) 5. Turn fine knob  to make the image clear. (Figure 12) (Suitable for sliding head) Or 1. Let objective 4X move into light path. 2. Turn one dioptor ring  to make “0” position in line with reticle  on eyepiece tube. (Figure 15) 3. Turn coarse knob  to find the image. (Figure 12) 4. Adjust anther eyepiece to make the image clear. If not, please adjust dioptor. (Figure 15) 5. Turn fine knob  to make the image clear. (Figure 12) (Suitable for gemel head) Figure 11 Figure 12 Figure 10

If the coarse adjustment is too tight or uncomfortable or image swiftly out of focal plane after focusing or the stage sleeks down, please use knob  to adjust. (Figure 13) Turn the knob  with the direction showed to tight the knob. And opposite direction for loosement. 3-5 Adjust diopter （Figure 14&15） 1. Turn one dioptor ring  to make it in line with reticle  on eyepiece tube. At this time, the position is “0”. Use objective 10X and adjust another diopter ring to make image clear. (Figure 14) (Suitable for sliding head) Or Turn one dioptor ring  to make “0”position in line with reticle  and make the image clear. Then observe through another eyepiece. If the image is blur, please adjust another diopter till the image clear. (Figure 15)  Diopter is ±5 and when it is in line with reticle , the number is the eye’s diopter.  Please remember own diopter for future using. (Suitable for gemel head) 7 Figure 14 Figure 15 Figure 13

Slide the board  with direction showed to make image through both eyepieces syncretic. (Figure 16) The interpupillary distance: 52-75mm  Please remember own interpupillary distance for future using. (Suitable for sliding head) Turn both prism houses to adjust interpupillary distance till the image through both eyepiece syncretic. The “。”  on eyepiece tube point to reticle , then the number is interpupillary distance. (Figure 17) Interpupillary distance: 50-76mm  Please remember own interpupillary distance for future using. (Suitable for sliding head) 3-7 Adjust center of condenser 1. Turn the condenser up-down knob  with the direction showed to make the condenser to top position. (Figure 18) 2. Slide 10X objective into light path to find image. 3. Turn the ring on view field diaphragm  to Min.. At this time, you may find image for the diaphragm. 4. Adjust the condenser holder knob to make the diaphragm image clear. 5. Adjust the adjusting-screw on condenser  to make the diaphragm image center. (Figure 19) 6. Open the view field diaphragm slowly. If the diaphragm image is always at center and inscribed with view field, then the condenser has been centered. 7. During operation, please open view field diaphragm a little to make the its image exscribed. 8 Figure 18 Figure 17 Figure 16

The view field diaphragm can prevent the light diameter into condenser in order to remove varied-light and strength the image. When the image of view field diaphragm is just outside of view field, the objectives can provide optimal performance to get the clearest image. 3-9 Aperture diaphragm (Figure 20&21) 1. Aperture diaphragm determines aperture number of illumination. Only when the aperture number for illumination and objective is matching, you can get the better resolution and contrast of image and to increase depth of field. 2. As the contrast of sample for microscope is very low, please turn the knob  during operation to let the arrowhead point to number chip which shows same magnification as objectives. That means adjust the aperture diaphragm of condenser to 70-80% of objective aperture. If necessary, take off the eyepiece and observe through eyepiece tube and adjust knob  till finding the image Figure 18. (Figure 20&21) ( shows aperture diaphragm and  shows objective edge in Figure 20 ) 9 Figure 20 Figure 21 Figure 19

1. Use objective 4X to find image. 2. Drop oil  on sample. (Figure 22) 3. Turn the nosepiece counter-clockwise and slide the objective 100X into light path. Then turn fine adjusting knob to make image clear.  Make sure no bubble inside oil because any bubble will affect image quality. A. Please take off eyepiece and open view field diaphragm and aperture diaphragm thoroughly, then observe through eyepiece tube to check bubble. (If the edge of the objective is round and bright, there is without bubble.) B. Please turn nosepiece softly and let the 100X objective swing the oil to wipe off the bubble. 4. After use oil, please clean the objective with 3:7 mixed alcohol and ether or dimethylbenzene on cloth. Also please wipe off the oil on sample.  Do not turn other objective into light path before wipe off oil on sample to avoid oil moisten objectives.  It will dissolve glue on prism if use more dimethylbenzene. 3-11 Use filter (Figure 23) Filter can make the background of image more suitable. No filter for the LED version. There are blue, green, yellow and neutral for selection. 3-12 Replace fuse (Figure 24) Put the power switch on “OFF” before replacing the fuse. Cut the power supply first, then screw off the fuse group from the fuse base with “-” screwdriver. And put a new fuse and screw on the fuse base. (Fig.24)  Specification: 250V，3.15A. 10 Figure 23 Figure 24 Figure 22

The light path selecting levercontrols the prismatic between binocular and trinocular. When the lever is in the most inside, light enters the binocular fully; when the lever  is in the most outside, the prismatic is 8:2 for binocular and trinocular. Usually, push the lever  to the most inside when using the binocular and push it to most outside when using the trinocular (TV or Photo). (Fig.25) 3-14 Set Video device (Figure 26) 1. Loose the lock-screw  on trinocular head and take off the cap  on third tube. (Fig. 26) 2. Take off the cap on TV adapter  and screw the adapter into third tube with the direction showed and lock it. 3. Loose the lock screw  on TV adapter and take off C-mount , and screw in CCD or vidicon. Then screw the whole adapter into TV adapter and lock the screw . 4. Pull the light path selecting lever  to the most outside for observation for binocular. Adjust the tube  till image clear 3-15 Set photo adapter (Figure 27/28) 1. Loose the lock-screw  on trinocular head and take off the cap  on third tube. 2. Set the photo adapter into third tube with the direction showed and lock the screw . (Fig. 27) 3. Loose the lock-screw  and take off photo tube. 4. Set 3.2X photo eyepiece  into the eyepiece hole  and put on photo tube , then lock on screw . 5. Observe through binocular till image clear, then pull the light path selecting lever  to the most outside. And operate under the manual of camera. 11 Figure 26 Figure 27 Figure 28

4-1 D1 series biological microscope specification 4-2 Performance for objectives 12 Optical system Color corrected optical system Head Sliding binocular head, 45°inclined, 360°rotatable Gemel binocular head, 30°inclined, 360°rotatable Gemel trinocular head, 30°inclined, 360°rotatable with prismatic: binocular100%, binocular/trinocular 80%/20% Eyepiece PL10X high point plan eyepiece, 18mm Nosepiece Build-in quadruple nosepiece Objective High contrast achromatic objective (4X, 10X, 40X, 100X) Plan achromatic objective (4X, 10X, 40X, 100X) Focusing Fine and coarse coaxial focusing with top limited for coarse and tightness adjustment. Moving range 28mm. Precision: 0.002mm Stage Dual mechanical stage 140*132mm with moving range 76*50mm, low adjustment Illumination 85~265V wildly power supply, transmitted normal illumination, 6V20W halogen bulb, adjustment of brightness Operation environment ●Indoor using ● Altitude: below 2000m ● Surrounding temperature: 5-40 (41-109) ● Max. humidity: 80% humidity for 31(88), 70% humidity for 34(93),60% humidity for 37(99),50% humidity for 40(104) ● Contamination: 2 (refer to IEC664) Item Magnification Numerical aperture (N.A) Conjugate distance (mm) Parfocus distance (mm) Cover glass (mm) Mag. Remark (ring color) 4X 0.10 195 45 0.17 Red 10X 0.25 195 45 0.17 Yellow 40X(S) 0.65 195 45 0.17 Blue High contrast achromatic objective 100X(S)(OIL) 1.25 195 45 0.17 White 4X 0.10 195 45 0.17 Red 10X 0.25 195 45 0.17 Yellow 40X(S) 0.65 195 45 0.17 Blue Plan achromatic objective 100X(S)(OIL) 1.25 195 45 0.17 White

Symptom Cause Remedy 1. Opticis View field diaphragm is not large enough Open the diaphragm more Condenser is too low Adjust condenser position Condenser is not center Center condenser  The lamp is not bright enough Light path selecting lever is not at position for binocular Pull it to position for binocular Nosepiece is not on the right position Turn it to right position  the edge of view field is dark or even Dust on lens(condenser, objective or eyepiece) Clean them Stain on sample Clean sample  stain inside of view field Stain on lens(condenser, objective or eyepiece) Clean them No cover glass on sample Add cover glass The cover glass is not the standard Please use standardδ0.17mm Sample is faced down Put it again Oil on (dry) objective Clean the objective No oil on objective (oil) Use oil Bubble inside oil Wipe off bubble Wrong immersion oil Use right one Aperture diaphragm is not opened correctly Adjust it Stain or dust on prism of eyepiece Clean it  image blur Condenser is too low Adjust it The sample is not fixed Fix it Nosepiece is not at right position Turn it to right position  one side of image is dark or image swing while focusing Condenser is not center Center condenser 13

Adjust it Diopter is not correct Adjust it  The eyes tired easily. The both view field are not superposition The both eyepieces are different Please use same one 2. Mechanics Cover glass faced down Put it again  image is not focused while using high Mag. Objective- The cover glass is not the standard Please use standardδ0.17mm Cover glass faced down Put it again The objective touched cover glass while turning from low Mag. to high Mag. The cover glass is not the standard Please use standardδ0.17mm  Coarse knob is too tight Tightness adjusting ring is too tight Adjust it to suitable  The stage skipped down slowly while observation Tightness adjusting ring is too loose Adjust it to suitable  The coarse knob can not be adjusted up The top limited screw locked Loose the screw a bit  The coarse knob can not be adjusted down The condenser holder is too low Rise it The sample is not set correctly Re-set it  The specimen can not be moved smoothly Specimen holder is not fixed Fix it 3. Electrics No power supply Connect it The bulb is not fixed correctly Set it correctly  The bulb is not light The bulb burnt out Replace bulb  The bulb burnt out usually The bulb is not standard Please use standard bulb The bulb is not standard Please use standard bulb  brightness is not enough Brightness adjusting knob is not used right Use it right The bulb will be burnt out soon Replace bulb  light flashes The wires are not connected all right. Connect them correctly 14