Sartorius ePET Operation & User’s Manual
Text of the manual
1. Intended Use .....................................................................................................2
2. Product Description .......................................................................................2
2.1
ePET® Single-Channel Pipettes...................................................2
2.2
ePET® Multichannel Pipettes .......................................................3
3. Pipette Tips ........................................................................................................3
4. Unpacking ..........................................................................................................3
4.1
Electrical Specifications ................................................................3
4.2
Charging the Pipette ......................................................................4
5. Pipette Parts and Materials .........................................................................5
6. Pipette Description .........................................................................................6
6.1
Controls ...............................................................................................6
6.2
Start Button .......................................................................................6
6.3
Direction Symbols ............................................................................6
6.4
Display ..................................................................................................7
6.5
Loading and Ejecting Tips .............................................................7
6.6
Optional Filters..................................................................................7
7. Programming the Pipette ............................................................................8
7.1
Mode Selection and Mode Recall ..............................................8
7.2
Setting Speeds ..................................................................................8
7.3
Pipetting Mode (P) ..........................................................................9
7.4
Multi-Dispensing Mode (d).........................................................10
7.5
Diluting Mode (dd) ........................................................................11
7.6
Mixing Mode with Pipetting or Diluting ...............................12
8. Pipetting Recommendations ....................................................................13
8.1
Dispensing with Blow-out ..........................................................13
8.2
Dispensing without Blow-out ...................................................13
8.3
Other Recommendations ............................................................14
9. Storage ..............................................................................................................14
10. Performance Testing ....................................................................................15
10.1
Testing the Performance .............................................................15
11. Maintenance ...................................................................................................17
11.1.
Cleaning and Decontaminating the Pipette ........................17
11.2
In-house Maintenance .................................................................17
11.3
Battery Replacement ....................................................................18
12. Troubleshooting .............................................................................................19
13. Warranty Information .................................................................................21
14. Recycling Instructions (WEEE) .................................................................21
15. Performance Specifications ......................................................................22
1. Intended Use
The ePET® pipette is intended to be used in liquid
handling applications for dispensing liquids in
the volume range of 0,2 μl to 5 ml. With ePET®
pipettes it is recommended to use Sartorius
Optifit Tips or SafetySpace™ Filter Tips to ensure
the best compatibility and performance.
This liquid handling device is designed and
manufactured to be used as general purpose
laboratory equipment. Before operating, it is
recommended to read this user manual, which
contains useful information, for example, for
good pipetting practice.
2. Product Description
Your new Sartorius ePET® electronic pipette
offers a cost-effective electronic pipetting
alternative by using a direct charging system,
which requires no charging stand. The self-
calibrating, microprocessor-based system
reduces the possibility for human error and
instrument contamination, by controlling all
piston movements. Also, some models offer
replaceable Safe-Cone Filters to help prevent
contamination and damage.
Its light weight and ergonomic controls take the
effort out of pipetting to help reduce the risk
of repetitive strain injuries (RSI) that commonly
occur in manual pipetting. All ePET® electronic
pipettes operate on the air-displacement principle
and use disposable tips.
2.1 ePET® Single-Channel Pipettes
Order
No.
Volume Range
µl
Increment
µl
Tip µl
710521ET
0.2 - 10
0.1
10
710011ET
5 - 100
1
200 , 350
710041ET
50 - 1200
5
1200
710501ET
100 - 5000
50
5000
All pipettes include universal AC-adaptor (EU, UK, US/JPN,
AUS, KOR and CHN plugs).
2.2 ePET® Multichannel Pipettes
Order No.
Volume Range µl
Increment µl
Tip µl
710201ET
8-Ch 0.2 - 10
0.1
10
710211ET
8-Ch 5 - 100
1
200 , 350
710221ET
8-Ch 25 - 250
5
350
710801ET
8-Ch 50 - 1200
10
1200
3. Pipette Tips
It is recommended that Sartorius Optifit Tips
or SafetySpace™ Filter Tips are used with
Sartorius pipettes. This ensures the best possible
compatibility and thus best possible accuracy
and precision, as these tips are designed for the
pipette in question.
Optifit Tips and SafetySpace™ Filter Tips are also
available as Low Retention Tips.
4. Unpacking
The ePET® pipette package contains the following
items:
1. Pipette
2. Grease
3. AC/DC Adapter (acts as a recharging unit)
4. Instructions for use
5. Filters and tweezers (pipettes > 10 µl)
6. ISO8655-6 compliant performance certificate
Make sure that all items are included and that no
damage has occurred during shipping.
NOTE! The pipette can only be charged with the
original AC/DC Adapter supplied with the pipette.
4.1 Electrical Specifications
Battery
- Rechargeable NiMH battery
- Charging time a maximum of 12 hours for an
empty battery
AC/DC Adaptor
- Input voltage and main plug according to
local requirements
- Output voltage 9 VDC
4.2. Charging the Pipette
An ON/OFF switch is located at the top of the
pipette. This switch protects the battery from
discharging while the pipette is not in use and
not connected to the charger.
1. Switch the pipette ON (the far left position).
2. Connect the charger to a compatible AC
outlet and to the pipette's charger port.
NOTE! The charging light will remain illuminated,
when properly connected to the AC/DC
charger.
3. If the pipette is new, or the battery charge
is low, keep the pipette connected to the
charging unit for 12 hours to fully charge the
battery before continuing use.
4. Display shows E Press the START button
twice and the pipette is ready for the default
pipetting at maximum volume or for program
changes.
NOTE! The pipette will charge in both ON and OFF
positions. Leaving the pipette switched ON while
charging will retain user settings.
Switching it OFF will reset the unit to default
settings.
NOTE! If the pipette is left ON for several days,
the battery will discharge. As a result the display
will turn off, and there will be no response from
the keyboard or START button. This is because the
charge of the battery is below operating level.
Switch ON the
pipette; the power
indicator light
comes on.
Connect the
charger to the
pipette's charger
port
(at the back)
(ASA/PC)
Start
button
(POM)
Handle
(ASA/PC)
Tip ejector
lever (POM)
Tip ejector
collar (PP)
Tip cone
10 μl (PVC)
100 μl (SS)
1200 μl (PPS)
5000 μl (PE)
Keyboard
(silicon keys)
Tip cone
housing
(ASA/PC)
Display
(glass)
Tip ejector
lever (POM)
Tip ejector bar
(PETP)
Start button
(POM)
Handle
(ASA/PC)
Keyboard
(silicon keys)
Tip cones
10-250 μl (sealing: TPU)
10-250 μl (PVDF)
50-1200 μl (PPS)
5. Pipette Parts and Materials
ePET® Multichannel
ePET® Single-Channel
Power ON/OFF
switch
Tip Ejector Lever
Control Keys
M
E
S
Battery
Compartment
Power Switch
Charger
Port
6. Pipette Description
The control and the programming of the ePET®
electronic pipette are performed using the
control keys and display.
6.1. Controls
- Power Switch
- Charger Connection
- Battery Compartment
Control Keys:
S Changes speed
M Moves to next mode
Increases volume or speed
Decreases volume or speed
E Confirms setting changes
Switches Mixing on / off
Start Button
- Triggers all mechanical operations
- Colour-coded for easy identification of
pipette volume
Tip Ejector Lever
- Squeeze to remove pipette tip(s)
6.2. Start Button
The START button triggers the aspiration and
dispensing operations according to the selected
operating mode. Only a quick click is required to
operate the button. If the START button is held
down, the piston will stop in the lowest position
until the button is released. This feature is
applicable to all modes of operation, except for
multiple dispensing (d).
6.3. Direction Symbols
These symbols indicate the direction in which the
piston moves upon pressing the START button.
The small RIGHT ARROW in the display means
that the next function is to aspirate the liquid.
The small LEFT ARROW, in turn, indicates the
dispensing function in accordance to the selected
operating mode.
The left side of the display is the status indicator.
It informs the user about parameters to be
programmed, functions to be performed and the
number of dispensings available.
The right side of the display is used for
programming and displaying the various volumes
needed in different operating modes.
6.5. Loading and Ejecting Tips
Original Sartorius tips are recommended for use
with Sartorius ePET® pipettes. Before fitting a tip
make sure that the pipette tip cone is clean. Press
the tip onto the cone of the pipette firmly, to
ensure an airtight seal. The tip is tightly loaded,
when a visible sealing-ring has formed between
the tip and the tip cone.
Sartorius ePET® pipettes are designed for simple
and light attachment and ejection of the tips. To
eject the tip, simply place the pipette over the
waste container and squeeze the tip ejector lever.
6.6. Optional Filters
The tip cones of some ePET® electronic pipettes
allow the use of a removable Safe-Cone Filter as
an option. The filter prevents liquids and vapours
from entering the pipette. The filter does not
affect the calibration of the pipette. Sartorius
filter-tweezers should be used to insert or remove
a Safe-Cone Filter to avoid contamination
through contact with it.
Order Information
Safe-Cone Filter
Order No.
Channels
Volume Range µl
Increment µl
Standard
Plus
710521ET
1
0.2 - 10
0.1
N/A
N/A
710011ET
1
5 - 100
1
N/A
N/A
710041ET
1
50 - 1200
5
721006
721016
710501ET
1
100 - 5000
50
721006
721016
710201ET
8
0.2 - 10
0.1
N/A
N/A
710211ET
8
5 - 100
1
721014
N/A
710221ET
8
25 - 250
5
721014
N/A
710801ET
8
50 - 1200
10
721006
721016
The use of tweezers
to insert or remove
a Safe-Cone Filter
Squeeze the tip
ejector lever, to
eject the tip
Visible seal when tip
is loaded tightly
7. Programming the
Pipette
Programming is performed using the six-key
keyboard and the LC-display. There are three
operating modes offering special functions and
varying speeds to choose from.
7.1. Mode Selection and Mode
Recall
1. Press M repeatedly to view the available
modes of the pipette.
2. Press E when the desired mode is
displayed. The pipette is ready for use in the
mode selected.
NOTE: The mode can only be activated when the
piston is in its home position (arrow-right is lit),
and not during an aspiration or dispensing cycle.
7.2. Setting Speeds
1. Press S to display the current aspiration
speed.
2. Press or until desired aspiration
speed is displayed (”5” Fast and ”1” Slow).
3. Press E to confirm selected speed. Display
shows the current dispense speed.
4. Press or until desired dispense speed
is displayed (”5” being Fast and ”1” Slow).
5. Press E to confirm speed selection.
NOTE: The speed may not be changed during
aspiration or dispensing cycles.
NOTE: The default speed is 3 for all speed
settings.
7.3. Pipetting Mode (P)
The pipette performs ordinary pipetting with
blow-out.
1. Press M to display
.
2. Press E to confirm the mode change.
3. Select the desired pipetting volume by using
to increase, and to decrease.
NOTE: While holding down or , the volume
display starts to scroll faster.
4. Press E to confirm selection.
5. Position the tip to aspirate and press the
START button.
6. Position the tip to dispense. Press the START
button. The tip is emptied with a blow-out
and is ready for the next pipetting.
Step 5 Aspiration
(Pipetting Mode)
Step 6 Dispensing
(Pipetting Mode)
7.4. Multi-Dispensing Mode (d)
In the Multi-Dispensing Mode (d), the pipette
performs repetitive dispensings of a selected
volume. During this operation, the desired volume,
plus the automatically selected excess volume, is
aspirated into the tip.
1. Press M to display
.
2. Press E to confirm the mode change.
3. Press or until the multi-dispense
volume is displayed.
4. Press E to confirm selection.
5. Press or until the desired number of
aliquots is displayed.
6. Press E to confirm selection.
7. Position the tip to aspirate and press the
START button. The * sign and ARROW LEFT
are lit to indicate the reset function.
8. Position the tip to discard priming excess and
press the START button.
9. To dispense, position the tip, press the START
button and repeat until the cycle is complete.
10. Finally, position the tip to discard any
remaining excess and press the START button
twice.
NOTE: Upon delivering the programmed volume,
it is also possible to continue to aspirate and
dispense the same volume without emptying in
between. To continue, keep the START button
pressed down and within one second the direction
of the arrow will change. Keeping the button
down, place the tip into the liquid again and the
sample is aspirated into the tip by releasing the
START button.
↑
↑
↑
Step 7 Aspiration
(Multi-Dispensing
Mode)
Step 8 Discarding
priming excess
(Multi-Dispensing
Mode)
Step 9 Dispensing
repeatedly (Multi-
Dispensing Mode)
Step 10 Discarding
excess (Multi-
Dispensing Mode)
7.5. Diluting Mode (dd)
In the Diluting Mode (dd), two different solutions
separated by an air gap are aspirated and then
dispensed together with an automatic blow-
out. The purpose of the air gap is to prevent
contamination while aspirating the second
volume, but it will not prevent the two liquids
from mixing in the tip.
1. Press M to display
.
2. Confirm the mode change by pressing E .
3. Select the desired diluent volume (volume 1)
by pressing or .
4. Confirm by pressing E .
5. Press or to select the sample volume
(volume 2) on the display.
6. Press E to confirm selection.
7. Position the tip to aspirate volume 1 and
then press the START button.
8. With the tip in the air, press the START
button again to aspirate air to form an air
gap.
9. Position the tip to aspirate volume 2 and
press the START button.
10. Finally, position the tip to dispense, and press
the START button.
Step 7 Aspiration
(Diluting Mode)
Step 8 Air aspiration
for air-gap
(Diluting Mode)
Step 9 Aspiration of
2nd fluid
(Diluting Mode)
Step 10 Dispensing
(Diluting Mode)
7.6. Mixing Mode with Pipetting or
Diluting (*)
In the Mixing Mode, the piston is automatically
moved up and down to mix the liquid in the
delivery vessel. The mixing time is controlled by
the START button.
1. Use M to select either
or
on the
display.
2. Press to switch on mixing. The display
should show either
or
.
NOTE: Pressing will alternately switch mixing
on and off.
3. Confirm the mode change by pressing E .
For Pipetting:
1. Press to increase, and to decrease.
2. Press E to confirm selection.
3. Position the tip to aspirate and press START
button.
4. Position to dispense and press the START
button.
For Diluting:
1. Select the desired diluent volume (volume 1)
by using or .
2. Confirm selection by pressing E .
3. Press or to select the sample volume
(volume 2) .
4. Confirm selection by pressing E .
5. Position the tip to aspirate volume 1, and
press the START button.
6. With the tip in the air, press the START
button to aspirate air for an air gap.
7. Position the tip to aspirate volume 2 and
press the START button.
8. Finally, position the tip to dispense and press
the START button.
1. Position the tip in the solution, then press
and hold the START button. The mixing is
done automatically as long as the START
button is held down.
2. Position the tip to dispense. Press the START
button twice.
NOTE: The mixing is done with about 70% of the
total volume.
8. Pipetting
Recommendations
By using the various operating modes and special
functions, several different liquid handling
procedures are possible. Modes P and dd
feature an automatic blow-out and others
leave the excess liquid in the tip. Follow the
recommendations below to ensure optimal
performance.
8.1. Dispensing with Blow-out
The P and dd modes have an automatic blow-out
function, followed by an immediate return of the
piston to the ”home” position. To avoid accidental
aspiration of the liquid back into the tip, it is
recommended that the dispensing is always done
above the surface of the liquid.
By holding the START button down, during
dispensing, the piston will stop in the lowest
position. This allows the tip to be placed against
the bottom or the wall of the container. Once
the liquid is dispensed, the tip can be removed
from the container and the START button can be
released.
8.2. Dispensing without Blow-out
The pipette will not perform the blow-out
function while using the d mode. Therefore,
it is recommended that dispensing is always
performed with the tip set against the wall or the
bottom of the container. The use of the d mode
is especially useful when pipetting small volumes
or solutions that have a tendency to foam, or
have a high viscosity.
↑
↕
Step 1 Mixing
(Mixing Mode)
Step 2 Dispensing
(Mixing Mode)
8.3. Other Recommendations
- Hold the pipette vertically and place the
tip a few millimeters into the liquid when
aspirating.
- Pre-rinse the tip by filling and emptying
the tip five times. This is important when
dispensing liquids which have a viscosity and
density different from water or a temperature
other than ambient.
- Check that the pipette, tip and liquid are at
room temperature.
- Avoid contaminating the tip cone.
- Connect the pipette charger when the pipette
is not in use. Switch the pipette OFF when not
in use, if it is not connected to the charging
unit.
- Never strike the tip cone against a tip tray
when mounting tips, as this can damage
several internal components.
- Do not drop the pipette or AC/DC-adaptor, as
this may cause excessive shock.
- Avoid exposing the unit to extreme
temperature changes, humidity and dust.
- Avoid rough handling. Moderate pressure is
all that is required when using the keyboard
or START-button.
- Avoid leaving the pipette laying on its side
with liquid in the tip. The liquid might leak
into the pipette's mechanism.
- Always dispense against the inside wall of the
receiving vessel. Remove the tip by drawing it
up along the inside wall.
- Change the filter on the tip cone regularly
(after 50 - 250 pipettings).
9. Storage
When not in use, it is recommended that the
pipette is stored on a Sartorius Linear stand,
n an d
oitis
h e ON po
t ni e
pip e tt
PET ®
e e
t h h
w ti
connected to the charger. The green charging
light should be lit.
Order No.
Item
725620
Linear Stand for all Sartorius pipettes
Linear Stand
10. Performance Testing
It is recommended, the performance of your
ePET® pipettes be checked regularly (e.g. every 3
months), and always after in-house maintenance.
However, the user should establish a regular
testing routine for their pipettes with regard
to accuracy requirements of the application,
frequency of use, number of operators using
the pipette, nature of the liquid dispensed and
the acceptable maximum permissible errors
established by the user. (ISO 8655-1.)
10.1. Testing the performance
Performance testing should take place in a
draught-free room between 15° and 30°C, kept
constant within +/- 0.50°C, and with the relative
humidity above 50%. The pipette, tips and the
test water should have stood in the test room for
a sufficient length of time (at least 2 hours) to
reach equilibrium with the room's conditions. Use
distilled or de-ionised water (grade 3, ISO 3696).
Use an analytical balance with a readability of
0.01 mgs. (ISO 8655-6.)
Weighing
1. Adjust the desired test volume Vs.
2. Carefully fit the tip onto the tip cone.
3. Fill the tip with test water and expel it as waste
five times to achieve humidity equilibrium in
the dead air volume.
4. Replace the tip. Pre-wet the tip by filling it
once with test water and expel it as waste.
5. Aspirate the test water, immersing the tip only
2-3 mm below the surface of the water. Keep
the pipette vertical.
6. Withdraw the pipette vertically and touch the
tip against the inside wall of the test water
container.
7. Pipette the water into the weighing vessel,
touching the tip against the inside wall of
the vessel just above the liquid surface at an
angle of 30° to 45°. Withdraw the pipette by
drawing the tip 8-10 mm along the inner wall
of the weighing vessel.
8. Read the weight in mgs (mi).
9. Repeat the test cycle until 10 measurements
have been recorded.
10. Convert the recorded masses (mi) to volumes
(Vi)
Vi = mi * Z Z = correction factor
(Table follows)
Air Pressure (kPa)
95
100
101.3
105
20.0
20.5
21.0
21.5
22.0
22.5
23.0
23.5
1.0028
1.0029
1.0030
1.0031
1.0032
1.0033
1.0034
1.0036
1.0028
1.0029
1.0031
1.0032
1.0033
1.0034
1.0035
1.0036
1.0029
1.0030
1.0031
1.0032
1.0033
1.0034
1.0035
1.0036
1.0029
1.0030
1.0031
1.0032
1.0033
1.0034
1.0036
1.0037
11. Calculate the mean volume (⊽) delivered:
⊽ = (∑Vi)/10
12. To evaluate conformity, calculate the
systematic error es of the measurement:
in μl: es = ⊽ - Vs Vs = selected test volume
or in %: es = 100 (⊽-Vs)/Vs
13. To evaluate conformity, calculate the random
error of the measurement:
as standard deviation s = ∑Σ(Vi - ⊽)2
n - 1
n = number of measurements (10)
or as a coefficient of variation CV = 100s/⊽
14. Compare the systematic error (inaccuracy) and
random error (imprecision) with the values
in the performance specifications (p. 49) or
the specifications of your own laboratory. If
the results fall within the specifications, the
pipette is ready for use. Otherwise, check
both systematic and random errors and, when
necessary, proceed to recalibrate (Chapter
9.2).
NOTE: Systematic error (inaccuracy) is the
difference between the dispensed volume and the
selected test volume. Random error (imprecision)
is the scatter of the dispensed volumes around
the mean of the dispensed volume. (ISO 8655-1.)
NOTE: Sartorius specifications are achieved in
strictly controlled conditions (ISO 8655-6). The
user should establish own specifications based on
the field of use and the accuracy requirements
placed on the pipette (ISO8655-1).
NOTE: This method is based on ISO 8655.
Σ
To maintain the best results with your ePET®
pipette, the cleanliness of every unit should be
checked daily. Particular attention should be paid
to the tip cone(s).
ePET® pipettes have been designed for easy
in-house service. However, Sartorius also provides
a complete repair and recalibration service,
which includes a service report and performance
certificate(s). Please return your pipette to your
local representative for repair or performance
test/recalibration. Before returning it, please
make sure that it is free of any contamination.
Please advise our Service Representative of any
hazardous materials, which may have been used
with your pipette.
NOTE: Check the performance of your ePET®
pipette regularly e.g. every 3 months and always
after in-house service or maintenance.
11.1. Cleaning and
Decontaminating the Pipette
Your ePET® pipette should be checked every
day for cleanliness. To clean and decontaminate
the outer surface of your pipette use ethanol
(70%), isopropanol (60%) or a mild detergent as
a cleaning agent.
Gently clean the surface of the pipette with a
moistened cloth and then wipe dry. Pay special
attention to the tip cone. Change the Safe-Cone
Filter if needed (Chapter 6.6.)
11.2. In-house Maintenance
1. Remove the tip ejector collar: Gently twist
the tip ejector collar anti-clockwise and slide
it off.
2. Using 70% ethanol, 60% isopropanol, or a
mild detergent, and a soft lint-free cloth,
disinfect the tip ejector collar and the tip
cone.
3. Unscrew, anti-clockwise, the tip cone and
remove it, exposing the piston. In the event
the piston sticks to the tip cone, remove the
piston with a pair of tweezers.
4. To avoid scratching the surface of the piston
use ethanol (70%), isopropanol (60%) or a
mild detergent, and a lint-free tissue when
cleaning the piston. Leave the parts to dry.
NOTE: For complete decontamiantion, place the
tip cone, tip ejector collar, piston, O-ring and
spring into a beaker containing 70% ethanol, 60%
isopropanol, or a mild detergent, and leave for at
least 30 minutes. Rinse the parts with distilled
water, then dry, preferably with warm air.
5. Apply a thin layer of grease to the piston,
using the grease provided. Do not use any
other grease. Check that no lint or particles
are on the surface of the piston. Avoid excess
grease, especially at the bottom of the piston.
6. Reassemble the pipette by screwing the
piston and tip cone into their places, replace
the tip ejector collar, allowing the ejector
handle connection to snap into the notch for
the ejector collar. Replace the filter if fitted.
7. Test the pipette by pressing the START-button
several times. Test the operation of the tip
ejector.
NOTE: Check the performance of your Sartorius
ePET® pipette regularly, e.g. every 3 months, and
always after in-house service or maintenance.
11.3. Battery Replacement
If the battery is not sufficiently charged for
proper operation of the pipette, follow these steps
for replacement of the battery.
1. Switch the unit “OFF”.
2. Remove the top two screws on the back of
the pipette and remove the battery cover.
3. Carefully remove the battery by lifting it
straight out of the holder.
4. Install the new NiMH-battery by pressing the
positive (+) end against the contact spring at
the bottom of the holder.
5. Replace the cover and the screws. Do not
overtighten the screws.
6. Dispose of the old battery appropriately.
Battery replacement
The ePET® electronic pipette has a built-in
monitoring program to control the performance
of each pipetting action. If the error message ER1
appears on the display, this means the pipette has
been unable to perform the attempted action
properly. If you receive the error message please
do the following:
1. Connect the pipette to the charger for 15
minutes.
2. Clear the error message from the display by
pressing E .
3. Press START button, which will set the pipette
to its home position.
NOTE: As this procedure will empty the tip, it is
recommended that you remove the tip before
resetting the pipette.
The occasional ER1 situations can be caused by
electrical outlets that have been switched off, or
if the pipette has been in the OFF position during
charging.
Repeated occurence of the the ER1 message is
caused by an internal error; a failure to complete
the execution of the pipetting. In which case, it
would be necessary to return the pipette to your
local Sartorius representative for repair.
Troubles, causes and solutions
s
Trouble
Possible cause
Solution
Droplets left
inside the tip
Unsuitable tip
Use original Sartorius tips
Non-uniform wetting of the
tip plastic
Attach new tip
Laeakage or pipetted
volume
too small
Tip incorrectly attached
Attach firmly
Unsuitable tip
Use original Sartorius tips
Foreign particles between
tip and tip cone
Clean the tip cone, attach
new tip
Instrument contaminated
Clean and grease O-ring and
piston, clean the tip cone (*)
Insufficient amount of
grease on piston and O-ring
Grease accordingly
O-ring not correctly
positioned or damaged
Change the O-ring
Incorrect operation
Follow instructions carefully
Calibration altered or
unsuitable for the liquid
Recalibrate according to
instructions
Instument damaged
Send for service
Push button
jammed or moves
erratically
Piston contaminated
Clean and grease O-ring and
piston, clean the tip cone
Penetration of solvent
vapours
Clean and grease O-ring and
piston, clean the tip cone
Pipette blocked,
aspirated volume too
small
Liquid has penetrated tip
cone and dried
Clean and grease O-ring and
piston, clean the tip cone
Tip ejector
jammed or moves
erratically
Tip cone and/or ejector
collar contaminated
Remove ejector collar,
clean with 75% ethanol,
isopropanol (60%) or a mild
detergent
13. Warranty Information
Sartorius products are meant to be used as
described in this user manual.
ePET® electronic pipettes are covered by a
warranty for two years against workmanship and
defects in materials excluding battery.
ANY WARRANTY WILL, HOWEVER, BE DEEMED
AS VOID IF THE FAULT IS FOUND TO HAVE
BEEN CAUSED BY MISTREATMENT, MISUSE,
UNAUTHORIZED MAINTENANCE OR SERVICE OR
NEGLIGENCE OF REGULAR MAINTENANCE AND
SERVICE, ACCIDENTAL DAMAGE, INCORRECT
STORAGE OR USE OF THE PRODUCTS FOR
OPERATIONS OUTSIDE THEIR SPECIFIED
LIMITATIONS, OUTSIDE THEIR SPECIFICATIONS,
CONTRARY TO THE INSTRUCTIONS GIVEN IN THIS
MANUAL OR WITH TIPS OTHER THAN THE
MANUFACTURER’S ORIGINAL ONES.
Each ePET® electronic pipette is tested before
shipping by the manufacturer. The Sartorius
Quality Assurance Procedure guarantees that the
ePET® electronic pipette you have purchased is
ready for use. Each ePET® electronic pipette is
CE marked, fulfilling the requirements of the EN
55014, 1993/EN 55104, 1995/ ISO 13485:2003 and
Directive (98/79 EC).
14. Recycling Instructions
(WEEE)
In compliance with European Directive, WEEE
(2002/96EC) on waste and reduction of
hazardous substances in electrical and electronic
equipments, this device must not be recycled as
unsorted municipal waste. Instead this device
must be collected separately in accordance to the
local recycling regulations.
Batteries should be disposed of in accordance
with local legal regulations. Do not dispose of
batteries with domestic waste.
The manufacturer's specifications (p. 49) are
guaranteed only when the manufacturer's
original tips are used. The manufacturer's
specifications should be used as guidelines when
establishing your own performance specifications
in accordance with ISO 8655.
Specifications
ePET® Single and 8-Channel Pipettes
Order No.
Channels
Volume
range µl
Test
Volume
µl
Systematic
Error %
Random
Error %
710521ET
1
0,2 - 10
10
5
1
0,90
1,00
2,50
0,50
0,70
1,50
710011ET
1
5 - 100
100
50
10
0,40
0,70
2,00
0,15
0,30
1,00
710041ET
1
50 - 1200
1200
600
120
0,40
0,70
2,00
0,15
0,30
0,60
710501ET
1
100 - 5000
5000
2500
500
0,50
0,80
0,80
0,15
0,20
0,30
710201ET
8
0,2 - 10
10
5
1
0,90
1,50
4,00
0,50
0,80
4,00
710211ET
8
5 - 100
100
50
10
0,50
0,70
2,50
0,15
0,20
1,30
710221ET
8
25 - 250
250
125
25
0,40
0,60
1,50
0,15
0,20
1,00
710801ET
8
50 - 1200
1200
600
120
0,50
1,00
3,00
0,15
0,20
1,20
1. 用途 ··········································································································· 24
2. 电子移液器 ································································································· 24
2.1.
ePET® 单道电子移液器 ···························································· 24
2.2.
ePET® 多道电子移液器 ···························································· 25
3. 移液器吸头 ································································································· 25
4. 打开移液器包装 ······················································································· 25
4.1.
电气规格 ······················································································· 26
4.2.
移液器充电··················································································· 26
5. 移液器材料 ································································································· 27
6. 移液器说明 ································································································· 28
6.1.
控制 ································································································· 28
6.2.
开始按钮 ······················································································· 28
6.3.
方向符号 ······················································································· 28
6.4.
显示 ·································································································29
6.5.
密封与退除吸头·········································································29
6.6.
安全圆锥过滤器 ········································································29
7. 移液器编程 ·································································································30
7.1.
模式选择与模式调出 ······························································30
7.2.
设置速度 ·······················································································30
7.3.
移液模式 (P) ·················································································31
7.4.
多次分液模式 (d) ······································································· 32
7.5.
稀释模式 (dd) ··············································································33
7.6.
移液后混合(*或+混合)模式 ··················································34
8. 移液建议······································································································ 35
8.1.
采用吹出模式进行分液 ·························································· 35
8.2.
不采用吹出模式进行分液 ····················································· 35
8.3.
其它建议 ······················································································· 36
9. 贮存 ··········································································································· 36
10. 性能测试 ······································································································ 37
10.1.
性能测试 ······················································································· 37
11. 维护 ···········································································································39
11.1.
清洗消毒 ·······················································································39
11.2.
内部维护 ·······················································································39
11.3.
电池更换 ·······················································································40
12. 故障排除 ·······································································································41
13. 保修信息 ······································································································43
14. 回收说明 (WEEE) ·······················································································43
15. 性能规格 ······································································································44
16. 售后服务 ······································································································44
移液器家族其他成员 ·············································································46
实验室赛多利斯其他产品 ···································································· 47
ePET® 电 子 移 液 器 的 量 程 范 围 覆 盖 从 0.2-
5000µl。推荐使用 Optifit 吸头或 SafetySpace™
吸头,最好的兼容性和性能。
这种液体处理装置的设计和作为通用制造的
实验室设备。操作之前,推荐阅读本用户手册,
更好的操作 ePET® 移液器。
2. ePET® 电子移液器
新型赛多利斯 ePET® 电子移液器采用不需要
充电支架的直接充电系统,提供了一种颇具
成本效益的电子移液替代方案。该自校正、
基于微处理器的系统通过控制活塞的所有运
动,减少了人为误差和仪器污染。此外,选
择的型号还提供可更换的吸头锥体过滤器,
有助于防止污染和损坏。
该电子移液器重量轻,带人机控制,使移液
毫不费力,可以减少手动移液经常引起的重
复性劳损 (RSI)。所有 ePET® 电子移液器根据
空气置换原理操作,并采用一次性吸头。
2.1. ePET® 单道电子移液器
产品编号
量程
(µl)
可变量
(µl)
吸头
(µl)
710521ET
0.2 - 10
0.1
10
710011ET
5 - 100
1
200, 350
710041ET
50 - 1200
5
1200
710501ET
100 - 5000
50
5000
所有的移液器包含有通用的AC-适配器(欧盟,英国,
美国/日本,澳大利亚,韩国和中国)。
2.2. ePET® 多道电子移液器
产品编号
通道数
量程
(µl)
可变量
(µl)
吸头
(µl)
710201ET
8
0.2-10
0.1
10
710211ET
8
5-100
1
200, 350
710221ET
8
25-250
5
350
710801ET
8
50-1200
10
1200
3. 移液器吸头
建 议 将 Sartorius Optifit 吸 头 或 SafetySpace™
滤芯吸头配合 Sartorius 移液器一同使用。这
些吸头是针对上述移液器设计的,因此能够
确保最佳的兼容性,从而获得最佳的准确性
和精确性。
Optifit 吸头和 SafetySpace™ 滤芯吸头同样具
有低吸附特性的型号。
4. 打开移液器包装
移液器包装包含下列物品:
1. 移液器
2. 润滑油脂
3. 交流 / 直流适配器 ( 其功能是作为充电装
置 )。
4. 使用说明书
5. 过滤器与过滤器镊子 ( 移液器 > 10 µl)
6. 符合 ISO 8655-6 的性能证书。
确保包装包含所有以上物品,并确保在运输
期间以上物品未发生任何损坏。
注!移液器只能采用与移液器一起供应的原
装交流 / 直流适配器进行充电。
充电 NiMH 电池
-
充电时间:空电池最多充 12 小时
交流 / 直流适配器
-
根据当地要求输入电压与电源插头
-
输出电压 9 VDC
4.2. 移液器充电
移液器顶部有一处“开 / 关 (ON/OFF)”开关。
此开关可保护移液器在未使用时不放电,移
液器不能与充电装置连接。
1. 打开移液器 (ON)( 远左端位置 )。
2. 只需将交流 / 直流适配器与兼容的交流电
插座连接,并与移液器连接。
注!当与交流 / 直流适配器 / 充电装置正确连
接时,充电灯一直亮着。
3. 如果移液器是新的,或电池电量低,在继
续使用前,将移液器与充电装置连接,充
电 12 小时,将移液器充满电。
4. 显示屏显示 E 按“开始(START)”按钮两次,
移液器准备就绪,处于最大移液体积的默
认移液位置或准备就绪可进行程序更改。
注!移液器在“开 (ON)”与“关 (OFF)”两个
位置都可充电。移液器处于“开”位置时,
若与充电装置连接,则保持用户选择的设置,
而当移液器处于“关”位置时,若与充电装
置连接,则将使移液器复位到默认设置。
注!若移液器处于“开”位置时几天不充电,
因为电池电压低于操作水平,显示将会消失,
键盘或“开始”按钮将无任何反应。
10 µl (聚氯乙烯)
100 µl (SS)
1200 µl (聚苯硫醚)
5000 µl (聚乙烯)
键盘
(硅胶)
旋转式分液头
(丙烯腈/
苯乙烯/
丙烯酸酯共聚
物/聚碳酯)
显示屏
(玻璃)
吸头退除杆
(聚甲醛)
吸头推出器架
(聚对苯二甲酸乙二酯)
开始按钮
(聚甲醛)
手柄
(丙烯腈/
苯乙烯/
丙烯酸酯共聚
物/聚碳酯)
键盘
(硅胶)
安全圆锥过滤器
10-250 µl (密封热塑性
聚氨酯弹性体)
10-250 µl (聚偏二氟乙烯)
50-1200 µl (聚苯硫醚)
ePET® 多道移液器
ePET® 单道移液器
充电开/关按钮
27
5. 移液器材料
切换混合开 - 关
“开始”按钮
-
启动所有机械操作
-
量程彩色标识
吸头退除器杆
-
挤压可以退除移液器吸头
6.2. 开始按钮
“开始”按钮启动吸液操作及根据选择的操
作模式进行分液的操作。操作此按钮仅需快
速按一次。若“开始”按钮保持按下位置,
则活塞将停在最低位置,直到松开“开始”
按钮。该特点适用于所有操作模式,多次分
液除外。
6.3. 方向符号
这些符号表明按下“开始”按钮时活塞移动
的方向。显示屏中的小“右箭头”指的是下
一功能是吸取液体。反之,“左箭头”指的
是根据选择的操作模式进行分液的功能。
建议采用原装赛多利斯吸头与 ePET® 电子移
液器一起使用。在安装吸头之前,确保移液
器吸头锥体洁净。将吸头紧套到移液器锥体
上,确保密封良好。当吸头与吸头锥体之间
形成可见密封环时,表明密封严密。
赛多利斯 ePET® 移液器的设计使吸头的连接
与退除简单轻松。退除吸头时,只需将移液
器放到废品容器上方,挤压吸头退除杆即可。
6.6. 安全圆锥过滤器
选定赛多利斯 ePET® 电子移液器的吸头锥体
允许采用可选可拆卸的安全圆锥过滤器。过
滤器避免液体与液体蒸汽进入移液器。过滤
器不影响移液器的校正。采用赛多利斯过滤
器镊子,避免用手接触污染的过滤器。
订购信息
安全圆锥过滤器
产品编号
通道数
量程 (µl)
可变量 (µl)
标准型
增强型
710521ET
1
0.2 - 10
0.1
N/A
N/A
710011ET
1
5 - 100
1
N/A
N/A
710041ET
1
50 - 1200
5
721006
721016
710501ET
1
100 - 5000
50
721006
721016
710201ET
8
0.2 - 10
0.1
N/A
N/A
710211ET
8
5 - 100
1
721014
N/A
710221ET
8
25 - 250
5
721014
N/A
710801ET
8
50 - 1200
10
721006
721016
注!只有当活塞处于原始位置 ( 右键标志亮 )
而不是处于吸液中或分液循环时,才能激活
模式。
7.2. 设置速度
1. 按
显示当前吸液速度。
2. 按
或
,直到吸液速度显示 (“5”快速,
“1”慢速 )。
3. 按
,确认速度选择。显示屏显示当前
分液速度。
4. 按
或
,直到要求的分液速度显示
(“5”快速,“1”慢速 )。
5. 按
确认速度选择。
注!在吸液或分液循环中间不能改变速度。
注!所有速度设置的默认速度是 3。
7.3. 移液模式 (P)
移液器执行通常的吹出移液。
1. 按
,以显示 P。
2. 按
确认模式更改。
3. 使用
增加,
减少,所需的移液体积。
注!按住
或
,体积显示开始快速滚动。
4. 按
确认选择。
5. 将吸头定位到吸液位置,按下“开始”按钮。
6. 将吸头定位到分液位置。按“开始”按钮。
吸头通过吹出清空,并为下一次移液做好
准备。
7.4. 多次分液模式 (d)
移液器执行所选体积的重复分配。在此操作
中,所需体积加上自动选择的过量体积被吸
到吸头中。
1. 按
,以显示 D。
2. 按
确认模式更改。
3. 按
或
,直到显示多次分液体积。
4. 按
确认选择。
5. 按
或
,直到显示要求的等分数量。
6. 按
确认选择。
7. 将吸头定位到吸液位置,按“开始”按钮。
* 标志与左箭头亮,表明复位功能。
8. 将吸头定位,排出多余液体,按下“开始”
按钮。
9. 为了分液,定位吸头,按“开始”按钮,
重复,直到完成循环。
10. 最后,将吸头定位,排出残留的多余液体,
按“开始”按钮两次。
注!在移取编程体积时,不带清空功能也可
以继续移取与分配相同体积。为了继续,保
持“开始”按钮处于按下位置,在一秒内,
箭头方向将改变。保持按钮按下,将吸头再
次放入液体中,通过松开“开始”按钮,样
品被吸入到吸头内。
7.5. 稀释模式 (dd)
在稀释模式 (dd) 中,移取被空气隔层分开的
两种不同溶液,然后采用自动吹出分液。空
气隔层的作用是当移取第二体积时避免污染,
但其不能避免两种液体在吸头内混合。
1. 按
,以显示 DD。
2. 按
确认模式更改。
3. 采用
或
,选择所需的稀释体积 ( 体
积 1)。
4. 按
确认选择。
5. 按
或
,选择显示屏上的样品体积 ( 体
积 2)。
6. 按
确认选择。
7. 将吸头定位到吸液体积 1 位置,按“开始”
按钮。
8. 吸头在空气中时,再次按“开始”按钮,
吸取空气隔层。
9. 将吸头定位到吸液体积 2 位置,按“开始”
按钮。
10. 最后,将吸头定位到分液位置,按“开始”
按钮。
7.6. 移液后混合 (* 或 + 混合 )
模式
活塞自动上下移动混合输送容器中的液体。
混合时间由“开始”按钮控制。
1. 使用
在显示屏上显示选择 P 或 DD。
2. 按
打开混合。显示应为P*
或DD*
。
注!按
将交替打开混合“开”与“关”。
3. 按
,确认模式更改。
对移液来说:
1. 按
增加,按
减少。
2. 按
确认选择。
3. 将吸头定位到吸液位置,按“开始”按钮。
4. 将吸头定位到分液位置,按“开始”按钮。
对稀释来说:
1. 采用
或
,选择所需的稀释体积 ( 体
积 1)。
2. 按
确认选择。
3. 按
或
,选择样品体积 ( 体积 2)。
4. 按
确认选择。
5. 将吸头定位到吸液体积 1 位置,按“开始”
按钮。
6. 吸头在空气中时,按“开始”按钮,吸取
空气隔层。
7. 将吸头定位到吸液体积 2 位置,按“开始”
按钮。
8. 最后,将吸头定位到分液位置,按“开始”
按钮。
注!混合采用总体积的大约 70% 进行。
8. 移液建议
采用不同的操作模式与特殊功能,可以进行
几种不同的液体处理方法。模式 P 与 dd 的特
点是自动吹出,而其它模式的特点是在吸头
内保留过量液体。
遵守以下建议,以确保获得最佳性能。
8.1. 采用吹出模式进行分液
P 及 dd 模式具有自动吹出功能,随后紧接着
活塞立即返回到“初始”位置。为了避免将
液体意外吸回到吸头,建议分液总是在液体
表面上方进行。
通过在分液期间一直保持“开始”按钮按下,
活塞将停在最低位置。这允许吸头靠容器底
部或容器壁放置。一旦液体被分配,吸头可
从容器取出,松开“开始”按钮。
8.2. 不采用吹出模式进行分液
当采用这种模式时,移液器将不执行吹出功
能。因此,建议分液总是在吸头靠容器壁或
底部时进行。当移取少量溶液或移取容易产
生泡沫或高粘度的溶液时,采用模式 d 特别
有用。
不要摔落移液器或交流 / 直流适配器,因
为这可能导致过度震动。
-
避免将移液器曝露在温度变化大、高湿度
与高尘埃环境中。
-
避免粗心操作。当使用键盘或“开始”按
钮时,只需适当按压即可。
-
避免在吸头内有液体时将移液器侧放,这
可能会使液体回渗到移液器内部。
-
总是紧靠接收容器壁移取。将吸头紧靠内
壁,上拉拿出吸头。
-
定期更换吸头上的过滤器 ( 移液 50-250
次之后 )。
9. 贮存
当移液器不使用时,建议将移液器放在移液
器支架上,处于“开”位置,与充电装置连接。
绿色充电灯应为亮。
产品编号
项目
725620
倾斜支架 (不带充电功能)
建议定期(如每 3 个月)测试赛多利斯移液
器的性能,在自行维护后必须测试性能。用
户应该根据移液器使用的精准度要求、使用
频率、移液器的操作人员数量、分配液体的
性质以及可接受的最大允许误差 (ISO 8655-1)
等来确定定期测试。
10.1. 性能测试
性能测试应该在无风的房间中进行,房间温
度为 15-30°C,波动为 ±0.5°C,相对湿度高于
50%。移液器、吸头以及测试水应该在房间
里放足够长的时间(至少 2 小时),达到和
房间条件的平衡。使用蒸馏水或者去离子水
(ISO 3696,等级 3)。使用根据 ISO 8655-6
标准的分析天平。
称重
1. 调整所需测试体积 VS。
2. 将吸头小心地安装到吸头圆锥上。
3. 将吸头注满测试用水,然后排出,重复 5
次,达到滞留空气体积的湿度平衡。
4. 更换吸头。将测试用水注满吸头进行预湿
润然后排出废弃。
5. 吸 取 测 试 水, 将 吸 头 浸 在 水 面 以 下
2-3mm。保持移液器垂直。
6. 垂直提起移液器,将吸头在测试用水的容
器侧壁上碰一下。
7. 将水排出到称量容器中,将吸头倾斜 30°
到 45°,刚好在液体表面的容器内壁上碰
一下。沿着称量容器的内壁提起移液器
8-10 mm,使最后一滴液体流下。
8. 读取以 mg 为单位的重量 (mi)。
9. 重复测量,直至记录 10 次测量值。
10. 将记录的质量 (mi) 乘以校正因子 Z 转化为
体积 Vi
Vi = mi * Z
Z = 修正系数
( 表如下 )
Z- 值 (µl/mg):
温度 (°C)
气压 (kPa)
95
100
101.3
105
20.0
20.5
21.0
21.5
22.0
22.5
23.0
23.5
1.0028
1.0029
1.0030
1.0031
1.0032
1.0033
1.0034
1.0036
1.0028
1.0029
1.0031
1.0032
1.0033
1.0034
1.0035
1.0036
1.0029
1.0030
1.0031
1.0032
1.0033
1.0034
1.0035
1.0036
1.0029
1.0030
1.0031
1.0032
1.0033
1.0034
1.0036
1.0037
11. 计算得到的平均容量 : =(Σ Vi)/10
12. 为了进行一致性分析,计算测量值的系统
误差 eS:
以 µl 表示:eS = – VS
VS = 选定的测试容量
或以 % 表示:eS = 100(
– VS)/VS
13. 为了进行一致性分析,计算测量值的随机
误差,将其作为标准偏差:
s
n - 1
=
∑(Vi - V)2
n= 测量次数(10)
或以变动系数表示 CV=100s/
14. 将系统误差(不准确度)和随机误差(不
精确度)与实验室要求的性能规范值比较。
注!系统误差是移取的容量和选定测试容量
之间的差异。随机误差是多次移取的液体容
量与其平均值的分散程度(ISO 8655-1)。
注!赛多利斯产品的技术参数是在严格控制
的条件下获得的 (ISO 8655-1)。用户应根据
移液器的应用领域以及对移液器的准确要求
确定可接受的最大允许误差 (ISO 8655-1)。
注!这种方法是基于 ISO 8655。
赛多利斯 ePET® 电子移液器要求定期进行清
洗,确保无故障操作。定期更换吸头圆锥过
滤器。
ePET® 移液器已经被设计为便于清洗和保养。
然而,赛多利斯还提供了一个完整的维修和
校准服务,包括服务报告和性能证书。请内
部维护或将您的移液器寄回测试 / 校准。在
寄出之前,请确保没有任何污染。请告知我
们的客服移液器被用于什么危险材料。
注!定期检查 ePET® 移液器(例如每 3 个月),
总是内部服务或维修后。
11.1. 清洗消毒
ePET® 电子移液器应该每天检查清洁情况。用
70% 乙醇,60% 的异丙醇或者温和型清洁剂
对表面去污。
用湿布轻擦移液器表面并拭干。请额外注意
吸头连件,如果需要的话请更换安全圆锥过
滤器。(6.6 章)
11.2. 内部维护
1. 取下吸头推出器套。轻轻地逆时针转动
吸头推出器,并将其滑下。
2. 使用 70% 的乙醇,60% 异丙醇或者温和型
清洁剂,用不起毛的软布给吸头推出件和
吸头锥体拭擦干净。
3. 逆时针旋松吸头锥体,并将其取下,曝露
活塞。活塞可能与吸头锥体粘结,若发生
这种情况,用镊子取下活塞。
4. 为了避免刮伤活塞表面,使用不起毛的软
布沾 70% 乙醇,60% 异丙醇或者温和型
清洁剂清洁活塞,并让它干燥。
盛有 70% 乙醇,60% 异丙醇或者温和型清洁
剂的烧杯中至少 30 分钟,采用蒸馏水冲洗部
件,然后最好是在暖风中干燥。
5. 采用提供的润滑油脂在活塞上薄薄地涂上
一层。不要使用任何其它润滑油脂。检查
活塞表面上无任何绒屑或颗粒。避免油脂
涂得太多,尤其是在活塞底部不要涂得太
多。
6. 将活塞与吸头锥体重新旋回其原位,安装
移液器,更换推出器环管,使推出器手柄
连接卡进推出器套的连接槽内。若安装了
过滤器的话,更换过滤器。
7. 按“开始”按钮几次,试验移液器。试验
吸头推出器操作。
注!定期检查赛多利斯 ePET® 的性能,如每
三个月进行一次,并在内部维护或维修之后
总是检查其性能。
11.3. 电池更换
若电池无法保持足够的电力来维持移液器的
正常操作,按照下述步骤更换电池。
1. 将移液器关上 (OFF)。
2. 取下移液器背部的两颗顶部螺钉,取下电
池盖。
3. 上抬使电池脱出电池座,小心取出电池。
4. 安装新 NiMH- 电池,正极端对准电池座
底部的接触弹簧
5. 重新放回电池盖与螺钉。螺钉不要拧得太
紧。
6. 适当处置电池。
赛多利斯 ePET® 电子移液器具有内部监控程
序,控制每次移液操作的性能。若错误信息
ER1 出现在显示屏上,意味着移液器无法正
确进行预定操作。若看到这种错误信息,请
按照下述方法处理:
1. 将移液器连接充电器充电 15 分钟。
2. 按
,清除屏幕上的错误信息。
3. 按“开始”按钮,设置移液器到其初始位置。
注!由于此步将清空吸头,建议重新安装移
液器前,取下吸头。
有时 ER1 情况可能会因切断的电源插座而起,
或为移液器在充电期间处于“关”位置。
重复出现 ER1 信息,是因为出现内部错误,
无法完成移液操作。移液器需要返回当地赛
多利斯代表处修理。
42
故障,可能原因和解决方案
故障
可能原因
解决方案
吸头内部残留液滴
吸头不合适
采用原装吸头
塑料湿润不均匀
连接新的吸头
泄漏或移取体积
太小
吸头连接不正确
连紧
吸头不合适
采用原装吸头
吸头与吸头锥体之间存在
外部杂质颗粒
清洁吸头锥体,连接新
的吸头
仪器污染
清洁和润滑O形环和活
塞,清理过滤圆锥(*)
活塞与O形环的润滑油涂
得不够
对活塞与吸头锥体进行清
洁与涂润滑油脂。进行相
应的润滑。
O形环不正确定位或损坏
更换O形圈
错误操作
仔细按照指示
校准或改变不适合的液体
根据指令调整
仪器损坏
送去维修
按钮卡住或移动
不正常
活塞污染
清洁和润滑O形环和活
塞,清理过滤圆锥
液体渗过吸头锥体
清洁和润滑O形环和活
塞,清理过滤圆锥
移液器堵塞,移取
体积太小
液体渗过吸头锥体,并
干燥
清洁和润滑O形环和活
塞,清理过滤圆锥
吸头推出器卡住或
不规律移动
吸头锥体污染
移除止推环,使用75%乙
醇,60%异丙醇或温和型
清洁剂进行清洁
提供 2 年的质保。
然而,若故障的产生被视为由误用、滥用、
未经授权的保养和维修或疏忽定期的保养维
护、意外损坏、错误的存放或将产品用于超
出其参数规格之外的操作、违背本手册中给
出的操作或使用非原厂吸头所造成,则不承
担保修责任。
每支赛多利斯 ePET® 在生产商发货之前都进
行了试验。赛多利斯质量保证程序保证您购
买的赛多利斯 ePET® 能够随时可用。
每 支 赛 多 利 斯 ePET® 是 CE - 标 记 的, 符 合
EN 55014, 1993 / EN 55104, 1995 / ISO 13485:
2003 及 IVD 指令 (98/79 EC) 的要求。
14. 回收说明 (WEEE)
根据欧洲指令 WEEE ( 2002/96EC) 关于电气电
子设备废物与减少有害物质的要求,本设备
不得按未分级的城市废物回收利用。相反,
该设备必须根据当地回收法规,独立回收。
电池必须根据当地法规丢弃,不能丢弃在普
通垃圾桶。
根据 ISO8655 确定自己的性能参数时,厂家
参数仅供参考。
规格
ePET® 单道和 8 道移液器
产品编号
通道数
量程 (µl)
测量体积
(µl)
系统误差
(%)
随机误差
(%)
710521ET
1
0,2 - 10
10
5
1
0,90
1,00
2,50
0,50
0,70
1,50
710011ET
1
5 - 100
100
50
10
0,40
0,70
2,00
0,15
0,30
1,00
710041ET
1
50 - 1200
1200
600
120
0,40
0,70
2,00
0,15
0,30
0,60
710501ET
1
100 - 5000
5000
2500
500
0,50
0,80
0,80
0,15
0,20
0,30
710201ET
8
0,2 - 10
10
5
1
0,90
1,50
4,00
0,50
0,80
4,00
710211ET
8
5 - 100
100
50
10
0,50
0,70
2,50
0,15
0,20
1,30
710221ET
8
25 - 250
250
125
25
0,40
0,60
1,50
0,15
0,20
1,00
710801ET
8
50 - 1200
1200
600
120
0,50
1,00
3,00
0,15
0,20
1,20
16. 售后服务
与移液一样,实验室还必须注意日常工作常
用的工具和设备的质量和性能。为使您的液
体处理工具确保连续的准确度和精确度,我
们提供广泛的优质服务,如质量控制、维修
服务、预防性维护和校准服务。定期做到这些,
您的移液器就能保持良好的工作状态并有助
于延长其寿命。
赛多利斯校准实验室于 2011 年 7 月 11 日正
式获得中国合格评定国家认可委员会 (CNAS)
的“检测与校准实验室”认可。
在这里,我们不仅可以提供赛多利斯移液器
的维修和校准服务,还可以提供赛多利斯以
外的其他品牌移液器的维修和校准服务 :
-
所有类型的移液器
-
所有规格的移液器
-
所有量程的移液器
维修热线:0512-66168157、66168159
邮箱:[email protected]
网址:www.pipettedoctor.cn
在我们的服务中心,您的移液器将会得到最
高标准的维修和校准服务。
以下是我们的优势:
-
专业的一站式服务
-
无需将不同品牌移液器寄回不同售后服务
中心
-
方便快捷,无需您费神
-
我们的服务合约为您节约维修成本
电话:400.920.9889 | 800.820.9889
[email protected]
www.sartorius.com.cn
eLINE®电子
移液器
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设计,让您轻
松完成移液。
mLINE®手动
移液器
舒 适 的 手 感
及 量 程 锁 设
计 带 给 您 轻
而 易 举 的 精
准。
Proline® Plus
手动移液器
优 良 的 耐 化
学性、出色的
性 能, 是 您
的不二选择。
全新低吸附
吸头
疏 水 表 面 减
少 液 体 在 吸
头 中 的 残 留
量, 节 省 珍
贵 的 试 剂 和
样品。
Optifit
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47
实验室
赛多利斯其他产品
实验室天平
微生物检测
实验室过滤/超滤
实验室纯水
Sartorius Liquid Handling Oy
Laippatie 1
00880 Helsinki
Finland
Phone +358.9.755.951
[email protected]
Sartorius Lab Instruments GmbH & Co. KG
Weender Landstrasse 94-108
37075 Goettingen
Germany
Phone +49.551.3080
Fax +49.551.308.3289
www.sartorius.com
赛多利斯科学仪器(北京)有限公司
Email: [email protected]
热线电话:400 920 9889 | 800 820 9889
上海
地址:上海市浦东新区金科路4560号1号楼北楼三层
邮编:201210
电话:+86.21.6878.2300
传真:+86.21.6878.2332 | +86.21.6878.2882
The status of the information, specifications and illustrations in this manual are subject to change. Sartorius
reserves the right to make changes to the technology, features, specifications and design of the equipment
without notice. All trademarks are Sartorius property unless otherwise stated. Patents granted or pending.
Publication No. SUL6016-e140501 • Order No. 85037-544-00 • Ver. 05 | 2014 • © Sartorius Liquid Handling Oy
Publication No. SUL6016av140501 • Order No. 85037-544-01 • Ver. 05 | 2014 • © Sartorius Liquid Handling Oy