VWR P4 Instruction Manual

Instruction Manual European Catalogue Number EU/UK/CH Plug: 634-1089 Version: 1.0.0 Issued: 24, June 2019

Europe VWR International bvba Researchpark Haasrode 2020 Geldenaaksebaan 464 B-3001 Leuven + 32 16 385011 http://be.vwr.com Made in China

Warning 1 Avoiding Electrical Shock 1 Package Contents 2 Unpacking 2 Installation 2 Intended use 2 Symbols and conventions 2 Product Specifications 2 Overview 3 Description 3 Getting Started 4 Turn On and Self-check 4 Important Guidelines 4 General Operating Instructions 4 Touch Screen Using Tips 4 Select Application 4 Basic Operation 5 Measurement Results Operation 6 Files Operation 7 Calibration and System Settings 8 Performance Verification 11 Operation 13 Photometry 14 Quantitation 15 Spectrum 19 Troubleshooting 22 Repair and maintenance 22 Daily Maintain 22 Spare Parts Replacement 22 User replaceable accessories and spare parts 25 Technical service 26 Warranty 26 Compliance with local laws and regulations 26 Equipment disposal 27 Functions 28 Installation 28 Install and Uninstall VWR Software Multi Wave Pro 28 Run VWR Software Multi Wave Pro 31 Introduction 32 Main Interface 32 Menus Bar and Tools Bar 33

33 Operation 36 Quantitative Analysis 36 Kinetics Analysis 38 Spectrum Scan 39 Multi-wavelength Analysis 40 DNA/Protein Analysis 41 Energy Scan 42 Other Functions 43 APPENDIX 1 43 APPENDIX 2 44

Please follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit. VWR recommends against the use of P4 Spectrophotometer. To avoid electrical shock  Do not open the device.  Disconnect the device from the mains supply before carrying out maintenance work or changing the fuses.  The inside of the device is a high-voltage area Danger!  Do not use the device if it is damaged, especially if the main power cable way is in any damaged or defective.  Repairs may only be carried out by the service technicians from your local VWR office and authorized contractual partners.  The device must be connected to a power outlet that has a protective ground connection.  If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Avoiding Damage to the Instrument  Do not allow any liquid to enter into the device.  Do not operate the device in a hazardous location or potentially explosive environment.

1PC on request Power Cord ( UK Plug ) 1PC on request Power Cord ( CH Plug ) 1PC on request Software CD-ROM 1PC N/A USB Cable 1PC N/A Instruction Manual 1PC N/A Dust Cover 1PC on request Unpacking Open the package, according to carefully check the packaging packing list items, if found inside the packaging are missing or damaged items please contact your local VWR office and authorized contractual partners. Installation Placement Place the instrument on the stable table carefully. Install printer (Optional) Check to confirm instrument power switch is turned off, connect the printer’s data cable to the Instrument’s serial/USB port. Connect the power cord Check to confirm instrument power switch is turned off, the power cord plug into two separate power interface and power supply socket apparatus. Intended use For research use only. Not intended for any animal or human therapeutic or diagnostic use. Symbols and conventions The following chart is an illustrated glossary of the symbols that are used in this manual. CAUTION This symbol indicates a potential risk and alerts you to proceed with caution^ CAUTION This symbol indicates the presence of high voltage and warns the user to proceed with caution CAUTION This symbol indicates risks associated with hot surfaces Product Specifications Optical System Single Beam Wavelength Range 190-1100nm Band Width 2nm Stray Light ≤0.05%T @ 220nm & 360nm Photometric Range 0 to 200%T, -0.3 to 3.0A, 0 to 9999C Wavelength Accuracy ±0.5nm Photometric Accuracy ±0.4%T or ±0.004A @ 1A

Memory 236KB Language English, French, German, Spanish, Portuguese, Chinese Display TFT Colour LCD (5”, Touch Screen) Interface USB-A, USB-B,RS-232 Measuring Procedure Photometry, Quantitation, Spectrum Scan Power Supply AC 100~240 V, 50~60 Hz Dimension 456×360×185 Weight 10.7kg Work Environment 15 to 35℃, 15 to 70% relative humidity Store Environment -10 to 50℃, 15 to 70% relative humidity This instrument is compliant to the European Directives on Low Voltage Directive 2014/35/EU, Electromagnetic compatibility 2014/30/EU. Restriction on use of Hazardous Substances RoHS 2011/65/EU and their amendments. Overview P4 Spectrophotometer used in Chemistry, Pharmaceuticals, Biochemical, Metallurgy, Light Industry, Textile, Material, Environments, Medical, Education and some other fields for Quality Control laboratories. Description Front View Touch screen Lid of the Compartment Pull Rod Lid of Lamp Room Right View USB Port (for USB Disk/ USB Printer) Power Switcher Rear View

Switch on the power. Self-check includes the following steps: Turn On Lamp → Locating Filter Disc → Locating Automatic Sample Holder (If Installed) → Get Dark Current → Locating Wavelength → Check Energy → Check System baseline. Important Guidelines  Reagents and dilution buffers can cause cauterization and other damage to health.  Samples (nucleic acids, proteins, bacteria cultures) can be infectious and cause serious damage to health.  During sample preparation, measuring procedures and maintenance and cleaning work, observe all local laboratory safety precautions (e.g. wear protective clothing and gloves, use of disinfectant) regarding the handling of sample material.  Dispose of measuring solutions and cleaning and disinfectant materials in accordance with the relevant local laboratory regulations. General Operating Instructions Touch Screen Using Tips The entire screen can be started with a touch. To make a choice, use your nails, fingertips, pencil, or stylus to press the screen. Don't press the screen with sharp objects (such as ball point). Select Application Main Interface, press the icon to select application.

Quantitation Establish the standard curve and measure the concentration of the sample. Spectrum Scan the sample in a wavelength range. File Manage files stored in the instrument or USB disk. System System calibration and setup. Performance verification Verify the performance of the instrument. Basic Operation Home Back to main interface. Return Back to the previous interface. / Page Up/Down Go to previous/next page.

Open Open result(s) from internal/USB memory. Save Save result(s) to internal/USB memory. Print Print result(s). Delete Delete selected result(s). Rename, Print and Delete Results Rename a Sample: List interface, press the area Name, key in the sample name (Up to 8 characters). Print the Measurement Report: List interface, press the icon . Delete sample(s): List interface, press the Check Box, and press the icon . Open Results Open: 1. List interface, press the icon . 2. Press the icon internal memory/USB memory to select the memory which the file saved.

Save Results Save: 1. List interface, press the icon Save. 2. Press the icon / to select the Internal/USB memory which the file to save. 3. Type in the file name, press the button Save. Files Operation Internal Memory Internal memory of the spectrophotometer. USB Memory USB extended mass memory. Copy Copy the selected file(s) from internal /USB memory to USB/internal memory. Export csv Export file(s) To *.csv format Export txt Export file(s) To *.txt format Delete Delete the selected file(s). Rename, Import, Export and Delete Files

File management interface, press the area Name, key in the file name (Up to 8 characters). Copy File(s) From/To Internal Memory/USB Memory: File management interface, press the Check Box, press the button （Need USB disk）. Export File(s) To *.csv Format File management interface, press the Check Box, press the button （Need USB disk）. Export File(s) To *.txt Format File management interface, press the Check Box, press the button （Need USB disk）. Delete File(s): File management interface, press the Check Box, and press the icon . Calibration and System Settings Select the icon in the main interface. Display options to calibrate the system and configure the basic instrument settings. Calibration Calibrate Start to do calibration. Select Tab Calibration in the System interface. Remove something in the measurement channel, close the sample chamber cover, select the item Dark current, Wavelength or System baseline, press the icon to do calibration. Settings of Light Source Tungsten lamp reset Reset the Tungsten lamp usage. Deuterium lamp reset Reset the Deuterium lamp usage. Select Tab Light source in the System interface. The light source information is displayed on the screen.

to turn on/off the Tungsten lamp/Deuterium lamp. Change the lamp switching point: Press the value of lamp switching point. Input the new value. Reset the lamp usage: Press the icon / to reset the Tungsten lamp/Deuterium lamp usage time. Important information 1. If only one of the light sources is used in for a long period of time, please turn off another light source to save energy. 2. If the lamp switching point is changed, the system baseline must be recalibrated. Edit Clock Accept Accept the new value. Select Tab Clock in the System interface. Press the value of year, month, date, hour, minute or second to change. Press the icon to accept new value. Memory Management

Format USB Memory Format the USB mass storage. Select Tab Memory in the System interface. The use of the internal and USB memory (If inserted) are show. Press the icon / to format internal memory/USB memory. Language Selection Accept Accept the new language. Select Tab Language in the System interface. Select a language, press the icon to change. General Options Select Tab General Options in the System interface. Beep: Press the icon to turn on/off the beep. Brightness: Press the icon / to decrease/increase the brightness of the LCD display. Close display: Press the icon to turn on/off. If turned on, the display will close automatically if no operation for 30 minutes. Sample holder: If you have a new automatic sample holder installed, you need to set

/ (AC-5: 5 cells automatic sample holder, AC-8：8 cells automatic sample holder). Restore Defaults Restore Restore the parameters to factory settings. Select Tab Restore defaults in the System interface. Select an item, press the icon to restore. Performance Verification Select the icon in the main interface. Display options to verify the performance of the instrument.

preheated for 30 minutes, and then re-measure dark current. Verifying Wavelength Accuracy and Wavelength Repeatability Select Tab Wavelength accuracy in the Performance verification interface. Standard Sample: Holmium oxide solution or equivalent filter Measurement: 1. Remove something in the measurement channel, close the sample chamber cover, press the wavelength value, type in the wavelength of measurement, press the button Zero; 2. Put the Standard Sample in the measurement channel, press the button Measure; 3. Repeat step 2 to do measurement three times. The difference between the average of the three measurements and the standard value is the single-point wavelength tolerance. The difference between the maximum and minimum values of the three measurements is single point wavelength repeatability; 4. Repeat step 1-3 to do measurement single-point wavelength tolerance one by one. The maximum value in the single-point wavelength tolerance is wavelength accuracy. The maximum value in the single-point wavelength reproducibility is wavelength repeatability. Verifying Photometric Accuracy and Photometric Repeatability Select Tab Photometric accuracy in the Performance verification interface. Standard Sample: NIST 930D or equivalent filter Measurement: 1. Remove something in the measurement channel, close the sample chamber cover, press the wavelength value, type in the wavelength of measurement, press the button Zero; 2. Put the Standard Sample in the measurement channel, press the button Measure; 3. Repeat step 2 to do measurement three times. The difference between the average of the three measurements and the standard value is the single-point photometric tolerance. The difference between the maximum and minimum values of the three measurements is single point photometric repeatability; 4. Repeat step 1-3 to do measurement single-point photometric tolerance one by one. The maximum value in the single-point photometric tolerance is photometric accuracy. The maximum value in the single-point photometric reproducibility is photometric repeatability.

Select Tab Stray light in the Performance verification interface. Standard Sample: 10g/L NaI solution or equivalent filter (220nm), 50g/L NaNo2 solution or equivalent filter (340 or 360nm) Measurement: 1. Remove something in the measurement channel, close the sample chamber cover, press the wavelength value, type in the wavelength of measurement; 2. Put the Reference in the measurement channel, press the button Zero; 3. Put the Standard Sample in the measurement channel, press the button Measure, the result is the stray light of this wavelength. Verifying Noise (0A) Select Tab Noise (0A) in the Performance verification interface. Standard Sample: None Measurement: 1. Remove something in the measurement channel, close the sample chamber cover, press the wavelength value, type in the wavelength 500, press the button Zero; 2. Press the button Measure, the result is the noise of this wavelength. Verifying Noise (0%T) Select Tab Noise (0%T) in the Performance verification interface. Standard Sample: Block Measurement: 1. Remove something in the measurement channel, close the sample chamber cover, press the wavelength value, type in the wavelength 500, press the button Zero; 2. Put the Block in the measurement channel, press the button Measure, the result is the dark noise of this wavelength. Verifying Stability Select Tab Noise in the Performance verification interface. Standard Sample: None Measurement: 1. Remove something in the measurement channel, close the sample chamber cover, press the wavelength value, type in the wavelength 500, press the button Zero; 2. Press the button Measure, the result is the noise of 500nm. Verifying Bandwidth Select Tab Dark Bandwidth in the Performance verification interface. Standard Sample: None Measurement: 1. Remove something in the measurement channel, close the sample chamber cover, press the wavelength value, type in the wavelength 656.1; 2. Press the button Measure, the result is the bandwidth. Operation Important information The cuvettes must be clear and there’s no remains of the samples on the surface of it. Only Silicon (Quartz) cuvettes are permitted to be used in the range of UV area.

1. Main interface, press the icon to start a Photometry application. Mode Switch measurement mode to %T, Abs or Energy. Wavelength Set measurement wavelength. Zero Do 0Abs/100%T. Read Measure sample and record the result. List View the result(s) list. Increase/Decrease Increase/Decrease the gain of signal. Only for Energy mode. 2. Press the icon to switch to the measurement mode. Abs Measure absorbance value of the sample(s). %T Measure transmittance value of the sample(s). E Measure energy value of the sample(s). 3. Press the icon to set wavelength, key in the measurement wavelength. 4. Put the reference in the measurement channel, press the icon to do zero. 5. Put the sample in the measurement channel, press the icon to measure a sample and record the result. 6. Press the icon to browse the result(s).

1 Main interface, press the icon to start a Quantitation application. 2 Establish Method 2.1 Quantitation interface, press the button Establish method.

measured wavelength 1 A=A1-m*A2: Absorbance is equal to the difference between the absorbance value of the measured absorbance at the wavelength 1 and the wavelength 2, m is the Coefficient A=A1/A2: Absorbance is equal to the ratio of the measured absorbance value of the measured wavelength 1 and 2 Wavelength 1 Measurement wavelength 1 Wavelength 2 Measurement wavelength 2 Fitting LIN-0: Linear to zero LIN: Linear. QUA: Quadratic. Unit - (No Unit), %, ppm, ppb, g/L, mg/L, μg/L, ng/L, g/dL, mg/dL, μg/dL, mg/mL, μg/mL, ng/mL, μg/μL, ng/μL, mol/L, mmol/L, IU, Custom(User input, Up to 8 characters). Calibration Coe K: Input equation coefficient. Std M: Measure standard sample(s) Std I: Input standard sample(s) Standard quantity Standard sample number (Up to 10) 2.2 Press the item to set measurement parameters. 2.3 After all the parameters are set up, press the button Next to start establishing the standard curve. If the item Calibration is set to the parameter Coe K, Std M or Std I, please refer to 2.3.1, 2.3.2 or 2.3.3. 2.3.1 Input equation coefficient to establish standard curve. (1) Input equation coefficient K0 ~ K3. Press button Next. 2.3.2 Measure standard sample to establish standard curve (1) Put the reference in the measurement channel, press the button Zero to do zero.

(3) Repeat step 3.3 to measure other of the standard samples. (4) Press the item to input concentration of standard samples, press the button Next. 2.3.3 Input standard sample to establish standard curve (1) Press the item Abs and Conc to input absorbance and concentration of standard samples, press the button Next. 2.4 Finished establish method. Press the button Save to save the method, press the button Measure to accept the new method and go to the measurement interface, Press the button Finish to exit.

Method Select measurement method. Zero Do 0Abs/100%T. Read Measure the sample and record the result. List View the result(s) list. 3.2 Press the icon to select method.

3.4 Press the button Measure to accept the new measurement method and back to measurement interface. 3.5 Put the reference in the measurement channel, press the icon to do zero. 3.6 Put the sample in the measurement channel, press the icon to measure a sample and record the result. 3.7 Press the icon to browse the result(s). Spectrum Spectrum mode is used to scan the absorbance or transmissivity of the sample in a wavelength range. 1. Main interface, press the icon to start a Spectrum application.

Zero Scan baseline. Read Scan the sample and draw curve. Stop Stop scanning. List View the result(s) list. 2. Press the icon to setup the measurement parameters. Start wavelength Scan start wavelength End wavelength Scan end wavelength Step Scan interval: 0.1,0.2, 0.5, 1.0,2.0, 5.0,10.0 nm Speed HS: High speed MS: Medium speed LS: Low speed Photometry mode Abs: absorbance %T: transmissivity Y minimum Minimum ordinate

and back to measurement interface. 4. Put the reference in the measurement channel, press the icon to scan baseline. 5. Put the sample in the measurement channel, press the icon to scan a sample and record the result. 6. Press the icon to browse the curve and result(s). Scale Set the Coordinate value. Left Moves the cursor to the left point(peak) to point(peak). Right Moves the cursor to the left point(peak) to point(peak). Mode %T Change the mode to %T. Mode Abs Change the mode to Abs. Peak Change the search mode point/peak.

Problem Cause Solution Power on, no response Power cord connection is not reliable Improve connectivity Fuse burning Replace fuse Measurement uncertainty Glass cuvettes used in UV Range Use quartz cuvettes Sample is not Stable Improve the sample The concentration of sample is too high Diluted sample Power Supply Voltage Low or not Stable Improve the Power Supply Lamp damage or lamp life maturity Replace lamp Dark Current Error when self- check The lid of the compartment is open during self-check Close the lid, restart System Calibrate Failed Something block the Light path Remove it, calibrate again Measurements inaccurate Cuvettes were contaminated Clean cuvettes Samples were contaminated Improve samples Worse matching of the cuvettes Improve the matching of the cuvettes Dark current error Resample dark current Repair and maintenance Daily Maintain Check the compartment After measurement, the cuvettes with sample solutions should be taken out of the compartment in time. Or the volatilization of the solution would make the mirror go moldy. Users must pay more attention to the corrosive sample and liquid easy to volatilize. Any solution remains in the compartment should be wipe off immediately. Surface clean The cover of the instrument is with paint. Please use wet towel to wipe off the drips on the surface immediately. Organic solution is forbidden to be used to clean the cover. Please wipe off the dirt on the cover timely. Clean the cuvettes After every test or after a solution change, the cuvettes should be cleaned carefully, or the remains on the surface would cause measuring error. Spare Parts Replacement Replace the fuse Danger! Be sure to switch off the power and unplug the socket before replacement! 1. Tools preparation Prepare a 3×75 Flat Blade screwdriver. 2. Switch Off the power supply Switch off the power supply, and unplug the socket. 3. Take out the Fuse Seat Push the fuse case by using the screwdriver, and turn it counterclockwise, the fuse seat will pop out when released.

Pick out the spare fuse (3.15A/250V) and replace it. 5. Reset the fuse seat Replace the fuse seat in the power socket. Push the fuse case by using the screwdriver, and turn it clockwise, the fuse seat will be locked when released. 6. Switch on the power Plug the socket and switch on the power. Replace lamps Hot！ Wait 20 minutes before open the lamp chamber after power off to avoid scald! 1. Tools preparation Prepare a 6×150mm Flat Blade screwdriver and a pair of glove. 2. Power Off Switch off the power supply and unplug the socket. 3. Open the cover Loosen the indicated two screws and remove the lamp cover. 4. Replace the D2 lamp Unplug the connector(No. 1) .Unscrew the 2 screws on the D2 Flange (No.2) and remove the D2 lamp. Draw on the cotton glove and replace a new lamp. Fix the 2 screws and plug the connector again.

The Tungsten lamp is equipped with a blue-grey silicon coating by manufacturer. This coating is only a transport safety device. It can be removed with the first exchange of lamp. Pull out the defected W lamp and draw on the cotton glove. Insert the new W lamp as deep as possible on the lamp seat. Tungsten lamp 6. Adjust the position of the W lamp Switch on the power(the Switch Mirror should be placed to the position as indicates). Observe the entrance facular, and it should in the center of the entrance hole. If the facular deviate to Left or Right, then loosen the two screws and move the lamp seat to Left or Right until it focus on the center of the slot. Then fix the screws.

User replaceable accessories and spare parts Description Quantity Cat. No. CELL HOLDER, 4-CELL, 10MM 1PC 634-6003 CELL HOLDER, 4-CELL,10 TO 50MM 1PC 634-6004 CELL HOLDER, 4-CELL, 10 TO 100MM 1PC 634-6005 CELL HOLDER, FOR CYLINDRICAL CELL 1PC 634-6006 CELL HOLDER, WATER-JACKETED, 1 CELL, 10MM 1PC 634-1135 CELL HOLDER FOR MICRO CELLS,BEAM HEIGHT:15MM 1PC 634-0687 CELL HOLDER FOR TEST TUBES, D=16mm 1PC 634-1133 CELL HOLDER,5-POSITION AUTO CELL CHANGER, 10 TO 100MM 1PC 634-1136 CELL HOLDER,8-POSITION AUTO CELL CHANGER, 10MM 1PC 634-6010 CELL HOLDER, SOLID SAMPLE 1PC 634-6011 CELL HOLDER, WATER-JACKED, 4 CELL, 10MM 1PC 634-1134 CUVETTE, SQUARE.GLASS,10MM 4PCS 634-6013 CUVETTE, SQUARE.GLASS,20MM 2PCS 634-6014 CUVETTE, SQUARE.GLASS,30MM 2PCS 634-6015 CUVETTE, SQUARE.GLASS,50MM 2PCS 634-6016 CUVETTE, SQUARE.GLASS,100MM 1PC 634-6017 CUVETTE, SQUARE.QUARTZ,10MM 2PCS 634-6018 CUVETTE, SQUARE.QUARTZ,20MM 2PCS 634-6019 CUVETTE, SQUARE.QUARTZ,30MM 2PCS 634-6020 CUVETTE, SQUARE.QUARTZ,50MM 2PCS 634-6021 CUVETTE, SQUARE.QUARTZ,100MM 1PC 634-6022 CELL,QUARTZ, 100UL, 10MM, BEAM HEIGHT:15MM 1PC 634-0688 CELL,QUARTZ, 200UL, 10MM, BEAM HEIGHT:15MM 1PC 634-0689 CELL,QUARTZ, 500UL, 10MM 1PC 634-6025 FLOW CELL, 5 MM, GLASS, BEAM HEIGHT:15MM 1PC 634-0690 FLOW CELL, 10 MM, GLASS, BEAM HEIGHT:15MM 1PC 634-0691 FLOW CELL, 20 MM, GLASS, BEAM HEIGHT:15MM 1PC 634-0692 FLOW CELL, 30 MM, GLASS, BEAM HEIGHT:15MM 1PC 634-0693 FLOW CELL, 5 MM, QUARTZ, BEAM HEIGHT:15MM 1PC 634-0694 FLOW CELL, 10 MM, QUARTZ, BEAM HEIGHT:15MM 1PC 634-0695 FLOW CELL, 20 MM, QUARTZ, BEAM HEIGHT:15MM 1PC 634-0696 FLOW CELL, 30 MM, QUARTZ, BEAM HEIGHT:15MM 1PC 634-0697 PELTIER UNIT, A-1101 1PC 634-1140 SIPPER UNIT WITHOUT TEMP. CONTROL, A-1001 1PC 634-1138 SIPPER UNIT WITH PELTIER TEMP. CONTROL, A-1201 1PC 634-1139 PRINTER, THERMAL PRINTER, RD-TH32-SC 1PC 634-1137 THERMAL PAPER 1PC 634-6043 LAMP, HALOGEN, 12V/20W 1PC 634-1142

1PC 634-1143 FUSE, 3.15A/250V 1PC 634-0651 SOFTWARE CD-ROOM 1PC 634-1141 Technical service Web Resources Visit the VWR website at www.vwr.com for: • Complete technical service contact information • Access to the VWR Online Catalogue, and information about accessories and related products • Additional product information and special offers Contact us For information or technical assistance contact your local VWR representative or visit. www.vwr.com. Warranty VWR warrants that this product will be free from defects in material and workmanship for a period of two (2) years from date of delivery. If a defect is present, VWR will, at its option and cost, repair, replace, or refund the purchase price of this product to the customer, provided it is returned during the warranty period. This warranty does not apply if the product has been damaged by accident, abuse, misuse, or misapplication, or from ordinary wear and tear. If the required maintenance and inspection services are not performed according to the manuals and any local regulations, such warranty turns invalid, except to the extent, the defect of the product is not due to such non- performance. Items being returned must be insured by the customer against possible damage or loss. This warranty shall be limited to the aforementioned remedies. IT IS EXPRESSLY AGREED THAT THIS WARRANTY WILL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND IN LIEU OF THE WARRANTY OF MERCHANTABILITY. Compliance with local laws and regulations The customer is responsible for applying for and obtaining the necessary regulatory approvals or other authorisations necessary to run or use the Product in its local environment. VWR will not be held liable for any related omission or for not obtaining the required approval or authorisation, unless any refusal is due to a defect of the product.

disposed of with unsorted waste. Instead it's your responsibility to correctly dispose of your equipment at lifecycle -end by handling it over to an authorized facility for separate collection and recycling. It's also your responsibility to decontaminate the equipment in case of biological, chemical and/or radiological contamination, so as to protect from health hazards the persons involved in the disposal and recycling of the equipment. For more information about where you can drop off your waste of equipment, please contact your local dealer from whom you originally purchased this equipment. By doing so, you will help to conserve natural and environmental resources and you will ensure that your equipment is recycled in a manner that protects human health. Thank you

Quantitative Analysis  Provide 2 methods to establish a Standard Curve;  Up to 20 standard samples to establish a new curve or input the coefficients to make a standard curve;  There are three methods, which are Linear zero , Linear and Square to fit the standard curve. Kinetics Analysis  We can choose the Time Intervals(0.5, 1.0, 2.0, 5.0, 10.0, 30.0 or 60.0 Seconds);  You can choose different Photometric Mode to display the curve (Transmittance-Time & Absorbance-Time). Spectrum Scan  You can choose the Scan Intervals(0.1, 0.2, 0.5, 1.0, 2.0 and 5.0nm);  You can choose the Photometric Mode to display the spectrum (Wavelength-Transmittance, Wavelength- Absorbance);  Automatically list spectrum peaks;  Mathematics and smooth. Multi-wavelength Analysis  Up to 20 wavelengths to measure a sample. DNA/Protein Analysis  We provide 2 methods;  You can input the coefficients by yourself. Energy Scan  You can switch the light source point(W Lamp, D2 Lamp or automatically switch);  You can choose the scanning intervals(0.1,0.2,0.5,1.0,2.0 and 5.0);  You can set the gain(1 to 8 times);  Automatically list spectrum peaks; Installation We will show you how to install VWR Software Multi Wave Pro on your PC in this chapter. Install and Uninstall VWR Software Multi Wave Pro PC System Requirements  Pentium or above PC;  CD-ROM;  USB Ports.  512MB Memory(1GB or Above is strongly recommended);  50MB or above hard disc Space;  Microsoft Windows XP/Vista/7/8/10. Install VWR Software Multi Wave Pro 1. Put VWR Software Multi Wave Pro disc in the CD-ROM; 2. Double Click to open the CD-ROM, and then, double click Setup.exe which is under the root directory of CD to start installation , “Welcome” form appear, click Next;

4. Choose install path, then click Next to copy files to Hard disc; 5. Unplug the USB cable if connected before, click Next; 6. After finished copy all files of VWR Software Multi Wave Pro, it will start install the USB Drive. Click Next;

8. Click Finish to finish install USB Drive; 9. Plug the USB cable, click Next; 10. Click Finish to finish all the setup.

1. Start→All Files→VWR Software→Uninstall VWR Software Multi Wave Pro 1.0, click Next to uninstall. 2. After all the files were removed, click Finish to end. Run VWR Software Multi Wave Pro  Double click the icon on desktop.  Start→All Files→VWR Software→VWR Software Multi Wave Pro 1.0. Set up Communication Port Important information Be sure that the USB line which is connecting PC with instrument is working and the spectrophotometer is at the standby state interface. After VWR Software Multi Wave Pro being started, then click Main Menu→Options, a dialog box of Options will display in the screen, click Search to automatically search the instrument’s communication port, after get the communication port, click OK to save setting. If you choose the option of Connect to Spectrophotometer When Start, the instrument will connect to the PC automatically when you run VWR Software Multi Wave Pro next time.

Click Main Menu→Options, pop-up Options window, click User’s Information tag, choose it and input relative user information, click OK to save settings, all this information will appear in your testing report. Set Data Format Click Main Menu→Options, a dialog box of Options will display in the screen, click Data Format tag, choose the displaying format, and click OK to save setting. Set Interface Style Click Main Menu→Options, a dialog box of Options will display in the screen, and click Interface tag. You can custom the style and color schemes of the interface as you like. Click OK to save setting. Connect to PC After enter the Main Interface, click in the Tool Bar to connect with PC. After connection, the icon is on the pitched on state, you can release PC if you click the icon again. Introduction We will introduce the VWR Software Multi Wave Pro in this chapter. Main Interface This is the main interface after start.

Menus Bar and Tools Bar provide three different ways to operate this software.  You can finish all the operation through Keyboard or Mouse.  Most of the functions can be finished through the Shortcut Bar.  Most of the functions can be finished through the menu by Right-hand button. General Operating Instructions Set Wavelength Click the icon on the Tools Bar to set wavelength. Input the value of wavelength in the frame of Set Wavelength, click Goto to confirm. Then the instrument will move the wavelength to the point you want, then it blanks automatically.

in the Tool Bar. New a Testing Main Menu→File→New, select a testing to click. Lists of the file’s type ▪ Quantity Analysis File *.qua ▪ Kinetics Analysis File *.kin ▪ Spectrum Scan File *.wls ▪ Multi-wavelength File *.mti ▪ DNA/Protein Analysis File *.dna ▪ Energy Scan File *.ens ▪ Standard Curve *.std Test a Sample Put sample into the light path, click the icon in the Tool Bar. Set Test Parameters Click the button of to set the parameters. Modify a Sample 1. Choose the data frame you want to modify or set the curve as current curve; 2. Pull the unknown sample in light path; 3. Click the icon in Tools Bar to re-measure it, and save the current result to substitute the original one. Delete a Test Result 1. Choose the data frame you want to modify or set the curve as current curve; 2. Click the icon in the Tools Bar to delete the data. Name a Sample 1. Select the line you want to rename. 2. Double-click Sample Name, input the sample’s name, and press Enter on the keyboard to confirm. Save a Testing Click the icon in Tools Bar, input the file name and click Save to confirm in the dialog box. Open a Testing Click the icon in Tools Bar, choose the file name you need in the dialog box, then click Open to confirm. Print Test Report Click the icon in Tools Bar, then set Print Parameters in the dialog box, press Print to confirm. Export Data to Excel (the Microsoft Excel should be installed) Click Main Menu→File→Export to Microsoft Excel, Excel will be started up, and data will be stored in it. Export Curve as bmp Picture Click Main Menu→File→Export Bitmap, input the file name and click save to confirm the dialogue box. Switch Display Mode Click the icon or in the Tools Bar to switch the display mode (Abs. or Transmit). Zoom Selected Area Click the icon in Tools Bar, choose the area you want to magnify, then you’ll see the magnified one, the Mouse’s cursor turns into the form of “+”. Click the icon again to cancel the current status. Modify Scale Click the icon on Tools Bar to display coordinate as you like. Auto Fitting Click the icon on Tools Bar to change the scale to appropriate value. Select as Current Curve

Change the Color of Current Spectrum Click Main Menu→Curve Operate→Choose the color as you like. Search Peaks Click the icon in Tools Bar to search the peaks, they labeled automatically on the curve, and the peaks will be listed in the Right Frame. You can set the Peak height by clicking icon. Mathematics Function Click the icons or in the Tools Bar, a new dialog box will display on the screen respectively. You can add, subtract, multiply or divide two spectrums.

much smoother. Click Main Menu→Spectrum→Spectrum Smooth. Operation We will introduce you how to use VWR Software Multi Wave Pro. Quantitative Analysis VWR Software Multi Wave Pro use Standard Curve to test the sample’s concentration at a fixed wavelength point. 1. Click the icon in the Tools Bar shortcut to create a new Quantitative Analysis. 2. Click the icon in the Tools Bar Shortcut to set the parameters of Quantitative Analysis.  Choose test method;  Input test wavelength in WL Frame;  Choose the Concentration Unit ;  Choose curve fitting method; Create Standard Curve Two methods are under your choice to create a new curve. Method 1: Coefficient

(2) Click Fit Method to choose Fit Curve method; (3) Input the curve equation’s coefficients in corresponding frames; (4) Click OK to finish setting. Method 2: Use Standard Samples to create a new curve (1) Click Standard Sampling option; (2) Click Standard Sample Quantity to choose the quantity(≤20); (3) Input the sample concentration in corresponding Frame; (4) Click OK to finish setting; (5) Pull the Reference into the light path and click the icon , then the system will move the wavelength to the one you just set, it blanks then; (6) Put 1# standard sample into the light path and move the cursor on the fist frame of Abs. The sample Absorbance value will appear in this frame after click in the shortcut toolbar; (7) Measure other standard samples in the same way as step (6) shows. The curve displays automatically when finish; You can modify the curve’s coordinates by Clicking the icon . You can also save the curve by clicking , and you can reload it by clicking next time. 3. Pull the unknown into the light path (if it is Automatic 8-cell Holder in the instruments, click to set the location of the relative cell first), Then click the icon, click in the shortcut toolbar to test, the test result will display in the data sheet.

1. Click the icon to begin a new test of Kinetics; 2. Click the button of to set the parameters; 3. Choose Photometric Display Mode, input the Wavelength, Display Time, Total Time, Intervals, Responsible Mode and Coordinates Values; 4. Click OK to finish and exit setting; 5. Pull the reference into the light path and click icon in the Tool Bar to do blank. 6. Pull the sample into the light path and click the button of to begin the test.You can click icon in the Tools Bar to cancel at any time.

1. Click the icon on the Tools Bar to start a new Spectrum scan; 2. Click the icon in the Tool Bar to set the parameters of Wavelength Scan; 3. Choose the Photometric Mode, Wavelength Range for Scanning, Intervals, Repeat Times, Response Mode, and Coordinate Range; 4. Click OK to finish and exit setting; 5. Put reference into the light path, click the icon in the Tool Bar, then the dialog box of Baseline displays in the screen, click Scan to scan baseline, click Cancel to interrupt scanning and exit the course; 6. Put the unknown sample in the light path, click the icon in the Tools Bar to start scanning, click to interrupt and cancel scanning.

1. Click the icon on the Tools Bar, a new form appears on the screen; 2. Click the icon on the Tools Bar to set the parameters; 3. Choose the quantity of the wavelength, you need input the value of each wavelength; 4. Click OK to finish and exit setting; 5. Pull the reference into the light path, click the icon in the Tools Bar to do blank; 6. Pull the sample into the light path, click to start test. And the test result will be listed in the data sheet.

1. Click the icon on the Tools Bar to begin a DNA/Protein Analysis; 2. Click the icon to set parameters; 3. Choose the measure method first. We provide you 2 methods to measure, and you can also definite the coefficients and wavelength by yourself; 4. Click OK to finish and exit the setting; 5. Pull the reference into the light path, click in the Tools Bar to do blank; 6. Pull the sample in the light path, click the icon to measure. The result will display in the data sheet.

1. Click Main Menu→File→New→Energy Scan to build Energy Scanning; 2. Click the icon on the Tools Bar to set parameter; 3. Select Lamp Switch Mode, Wavelength Range, Intervals, Repeat Times and Coordinate Range; ▪ Automatic Switch Mode---The instrument can choose D2 Lamp or W Lamp according to setting; ▪ Deuterium Lamp Mode---Using Deuterium Lamp in the whole wavelength; ▪ Tungsten Lamp Mode---Using Tungsten Lamp in whole wavelength. 4. Click OK to finish and exit setting; 5. Pull the unknown sample in the light path, click the icon in Tools Bar to start scanning, click to interrupt scanning and exit the course;

Turn On/Off W Lamp Click Main Menu→Device→Turn On/Off W Lamp to open or close W Lamp. Turn On/Off D2 Lamp Click Main Menu→Device→Turn On/Off D2 Lamp to open or close D2 Lamp. Setup Switch Point of Light Source Click Main Menu→Device→Setup Switch Point of Light Source, input the value of wavelength, and then click OK to confirm. Get Dark Current Click Main Menu→Device→Get Dark Current, the system will reget the dark current and the new data will replace the old one. Scan System Baseline Click Main Menu→Device→System Baseline, the dialogue box will display in the screen, you can click Open to choose the storage base line to test, be sure that nothing in the light path, you can click Scan to build system base line. You can click Cancel to interrupt and exit the course. You can choose Save System Base Line option; a dialogue box will display in the screen and click Save to save system base line for your after use. You needn’t to build system base line normally, only when it is a very long time that the instrument wasn’t be used or the environment changed much. Automatic 8-Position Changer (Accessories) Click the icon … to pull the correspond cell in the light path. Cell Calibration (Need install auto 8 cells Changer) Click Main Menu→Device→Cell Calibration,this operation for matching difference of all the cells selected. APPENDIX 1 Methods of Quantitative Analysis Single Wavelength Method： Abs.=A1

Three Wavelengths Method： Abs.=A1-(WL1-WL2)*(A2-A3)/(WL2-WL3)-A3 APPENDIX 2 Methods of DNA/Protein Analysis Method 1： CDNA=(A1-Aref)*f1-(A2-Aref)*f2 CProtein= (A2-Aref)*f3-(A1-Aref)*f4 Ratio=(A1-Aref)/ (A2-Aref) A1=A260nm,A2=A280nm, Aref=A320nm (Optional) f1=62.9, f2=36.0, f3=1552, f4=757.3 Method 2： CDNA=(A1-Aref)*f1-(A2-Aref)*f2 CProtein= (A2-Aref)*f3-(A1-Aref)*f4 Ratio=(A1-Aref)/ (A2-Aref) A1=A260nm, A2=A230nm, Aref=A320nm (Optional) f1=49.1, f2=3.48, f3=183, f4=75.8