BIO RAD HYDROTECH Instruction Manual

HydroTech Vacuum Pump

TABLE OF CONTENTS Safety ................................................................................................................................................ i Section 1.0 Introduction................................................................................................................... 1 1.1 Features ......................................................................................................................... 1 1.2 Physical Description ....................................................................................................... 2 Section 2.0 Set up and Basic Operation ........................................................................................ 4 2.1 Unpacking....................................................................................................................... 4 2.2 Set up ............................................................................................................................. 4 2.3 Basic Operation.............................................................................................................. 6 Section 3.0 Maintenance and Troubleshooting............................................................................. 7 3.1 Maintenance................................................................................................................... 7 3.2 Troubleshooting Guide ................................................................................................... 8 Appendix A. Approximate Gel Drying Times................................................................................... 9 Appendix B. Specifications ............................................................................................................... 10 Appendix C. Warranty and Ordering Information ........................................................................... 11 Figures 1. HydroTech Vacuum Pump.............................................................................................................. 1 Tables 1. Front View Physical Features......................................................................................................... 2 2. Rear View Physical Features ......................................................................................................... 3 3. HydroTech Set Up .......................................................................................................................... 4 4. HydroTech Operation...................................................................................................................... 6 5. Maintenance Guide ........................................................................................................................ 7 6. Troubleshooting Guide ................................................................................................................... 8 7. Suggested Drying Times for Single Percentage Gels.................................................................... 9

personnel. This instrument is intended for laboratory use only. This product conforms to the “Class A” standards for electromagnetic emissions intended for laboratory equipment applications. It is possible that emissions from this product may interfere with some sensitive appliances when placed nearby or in the same circuit as those appliances. The user should be aware of this potential and take appropriate measures to avoid interference. This instrument should not be modified or altered in any way. Alteration of this instrument will void the man- ufacturer’s warranty, void the safety and emission certification, and create a potential safety hazard for the user. Bio-Rad is not responsible for any injury or damage caused by the use of this instrument for purposes other than for which it is intended or by modifications of the instrument not performed by Bio-Rad or an authorized agent. 1. Always connect the unit to a power receptacle using a 3 prong plug. Always use a receptacle which has proper earth grounding and, preferably, ground fault protection. 2. Never enclose the unit in an unventilated space or closet. 3. The fluid circulated in the Hydrotech may be considered toxic and/or corrosive. Exercise appropriate caution when handling the unit, disconnecting lines or opening the reservoir. Note: This product conforms to EN55014 for electromagnetic emissions. It is possible that emissions from the product may interfere with some sensitive appliances when placed nearby or on the same circuit as these appliances. The user should be aware of this potential and take appropriate measures to avoid interference !

tional slab gel dryer. It uses water, not vacuum pump oil, so it is easy to use, environmentally friendly, and practically maintenance free. All parts are corrosion resistant, for long life and trouble-free operation. Figure 1. HydroTech Vacuum Pump 1.1 FEATURES The HydroTech Vacuum Pump features include: • No vapor or liquid trap required • No oil or expensive oil-related maintenance required • Continuous cooling that ensures steady vacuum • No expensive diaphragms to replace every other year • Free air capacity sufficient to seal two gel dryers Introduction 1 HydroTech Vacuum Pump

Feature Description 1. Vacuum Inlet Quick disconnect valve for connecting to the gel dryer’s vacuum line. Port 2. Vacuum Line Pre-assembled with 1/4” quick disconnect fitting and 1/4” vacuum tubing. 3. Two-way Prevents foaming when the HydroTech is operating but the gel dryer is not in use. Valve This valve is plumbed into the vacuum line. 4. Power On/ This switch can be set for 0 to 3 hours of operation or continuous operation. Off Timer 5. Vacuum Provides vacuum pressure indicator. This gauge is plumbed into the vacuum line. Gauge (optional; not shown) Introduction 2 HydroTech Vacuum Pump 1 4 2 3

Feature Description 1. Reservoir Storage tank for recirculating water. 2. Reservoir Cap Screw-on cap for water reservoir. The cap is ventilated to prevent pressure building up in the reservoir. 3. Reservoir Quick disconnect valve for draining the Reservoir. Drain Port 4. AC Power Three-prong AC power input. Connection 5. Locking Casters allow the HydroTech to be moved. Once in position, the casters can Casters be locked. 6. Drain Line Pre-assembled with 3/8” quick disconnect fittings and 3/8” tubing for draining the reservoir. Introduction 3 4 5 1 MAX MIN 3 2 6

The HydroTech Vacuum Pump is shipped fully assembled. To use the pump, simply fill the reservoir with water and attach the vacuum tubing from the gel dryer to the HydroTech’s inlet port. 2.1 UNPACKING Carefully inspect the shipping carton for any damage which may have occurred in shipping. Severe damage to a carton may indicate damage to its contents. If you suspect that the contents have been damaged, immediately file a claim with the carrier in accordance with their instructions before contacting Bio-Rad Laboratories. Cut the shipping straps and open the top flaps of the shipping box. Remove the foam inserts, and lift the top portion of the shipping box over the vacuum pump. The vacuum pump should now be fully accessible and can be easily removed from the bottom box. Check the contents of the box against the supplied packing list. Remove the plastic bag from the unit and inspect the unit for external damage. If any part is missing or damaged, contact Bio-Rad Laboratories immediately. 2.2 SET UP Follow the steps in Table 3 for setting up the HydroTech Vacuum Pump. Warning Do not continually operate the pump with less than the “MIN” level of water in the tank. It could damage the pump. Table 3. HydroTech Set Up Step Procedure Description 1. Fill the HydroTech reservoir Remove the reservoir cap and add deionized or tap to the MIN mark. water. The water level should reach the MIN fill line marked on the tank. Re-install the cap. Set Up and Basic Operation 4

HydroTech Set Up Step Procedure Description 2. Connect the AC power cord. Plug the AC power cord into the HydroTech power input module and into a nearby AC outlet. 3. Turn the Power On/Off switch This ensures the pump is fully primed and air bubbles to run the pump for two minutes. have been flushed out. 4. Turn off the pump and check the The level should be between the MIN and MAX marks water level in the pump reservoir. on the reservoir. If the level is below MIN, add water; if it is above MAX, drain water. 5. Connect the pre-assembled vacuum line supplied with the HydroTech pump. 6. Connect the vacuum tubing Note: Minimize the length of vacuum tubing between from the dryer to the the dryer and the vacuum pump to achieve the best pre-assembled vacuum line. system efficiency. 7. In an easily accessible stretch This valve keeps the pump under vacuum when the of the vacuum tubing, cut the dryer’s vacuum seal is broken. tubing and insert the two-way valve that’s included with the Warning: Always turn the valve to the Closed position HydroTech pump. when the dryer is not in use. The Closed position shuts off air flow to the vacuum pump and prevents the water in the reservoir from foaming. 8. If you purchased the optional This gauge displays the vacuum pressure. HydroTech vacuum gauge, cut the vacuum line and insert the gauge. Set Up and Basic Operation 5 a. Plug the quick disconnect fitting end of the pre- assembled vacuum line into the vacuum inlet port. b. Plug a barbed two-way connector (shipped with the Model 583 Gel Dryer) into the other end of the pre- assembled vacuum line.

is not in use; the vacuum pump then can continue operating without foaming. Table 4. HydroTech Operation Step Procedure Description 1. Turn on the Power On/Off Choose either “continuous on” by turning the knob Timer switch. counter-clockwise or “timer” by turning clockwise. Maximum timed operation is 3 hours. 2. Turn the vacuum line two-way This prevents reservoir foaming when the vacuum is valve to the Closed position. running but the gel dryer is not in use. 3. Position the gel in the gel dryer and seal the gel dryer. 4. Turn the vacuum line’s two-way The pump will now pull a vacuum on the gel dryer. valve to the Open position. 5. When the gel is dry, break the Lift the Sealing Gasket of the gel dryer. Keep the vacuum seal. vacuum pump running to prevent liquid in the vacuum line from flowing back into the gel dryer. 6. Turn the vacuum line’s two-way This prevents reservoir foaming when the HydroTech valve to the Closed position. is running but the gel dryer is not in use. 7. Turn off the HydroTech pump. Set Up and Basic Operation 6

3.1 MAINTENANCE The HydroTech pump’s reservoir water should be changed at least once a month or if frequent use of the HydroTech has caused the water level to reach MAX. Regular reservoir maintenance helps maintain pump performance and reduces foaming. To change the water, follow the steps in Table 5. Table 5. Maintenance Guide Step Procedure Description 1. Turn off the Vacuum pump. Do not connect the drain line unless the pump is turned off. 2. Connect drain line to the Firmly push the drain line quick connect fitting into the Reservoir Drain Port. reservoir drain port to open the drain valve. (See Table 2.) 3. Place drain line into a large Secure the open end of the drain line before turning on bucket or sink. the pump, or it may begin spraying. 4. Turn on the pump. Draining the reservoir requires approximately 30 seconds. Warning: Do not run the pump without water for extended periods of time. 5. Turn off the Pump. Disconnect the drain line by pushing the button on top of the reservoir’s drain port. The drain port will automatically close. 6. Refill the reservoir with water. Fill the reservoir to the MIN mark with deionized or tap water. Reinstall the cap. Maintenance and Troubleshooting 7

tance, call 1-800-4BIORAD in the U.S.A.; or contact your local Bio-Rad representative. Table 6. Troubleshooting Guide Indication Problem HydroTech Vacuum No power Pump will not start Reservoir is foaming Pump is running but not pulling vacuum Pump is pulling vacuum Reservoir is above the MAX level No vacuum in Vacuum line is blocked gel dryer Gel Dryer is blocked No vacuum from Quick connect fitting HydroTech Vacuum is clogged Pump No water in reservoir Foaming in reservoir Declining vacuum Ambient temperature over time (longer above 35°C gel drying time) Foaming in reservoir Maintenance and Troubleshooting 8 Action Check electrical source, power connections, and fuse. To replace the fuse, unplug the dryer. Then pry open the cover on the power switch and pull out the fuse holder. (See Appendix B for replacement fuse specifi- cations.) Check the connections in the vacuum line. Drain the reservoir and, using deionized or tap water, refill to the MIN mark. Drain the reservoir and, using deionized or tap water, refill to the MIN mark. Check the vacuum line for kinks, clogs or other block- ages. Make sure the two-way valve is open. Vacuum line may be kinked inside gel dryer. Refer to the gel dryer instruction manual. Check that the quick connect fitting is properly con- nected and not clogged. Make sure the reservoir is filled to the MIN level. Drain the reservoir and, using deionized or tap water, refill to the MIN mark. Run the pump in a cooler environment, or refill the reservoir with chilled water. Drain the reservoir and, using deionized or tap water, refill to the MIN mark.

The following table provides approximate drying times for various gel concentrations, given the gel dryer’s temperature and vacuum are kept at 80°C and 28” Hg, respectively. Table 7. Suggested Drying Times for Single Percentage Gels Acrylamide Concentration Slab Thickness Time at 80° C 3% to 10% 0.375 mm 30 min. 10% to 20% 0.375 mm 30 min. 3% to 10% 0.5 mm 40 min. 10% to 20% 0.5 mm 40 min. 3% to 10% 0.75 mm 40 min. to 60 min. 10% to 20% 0.75 mm 60 min. to 120 min. 3% to 10% 1.5 mm 45 min. to 60 min. 10% to 20% 1.5 mm 60 min. to 120 min. 3% to 10% 3.0 mm 60 min. to 120 min. 10% to 20% 3.0 mm 120 min. to 180 min. Approximate Gel Drying Times 9

After 10 hours > 28” Hg at 22° C (48.8 Torr) At 35° C RT > 26” Hg at start (99.6 Torr) Flow rate 60 Hz > 28 liters per minute 50 Hz > 21 liters per minute Fluid Capacity Minimum (MIN) 4 liters Maximum (MAX) 5 liters Function Modes Continuous or timed operation Timer Control 0 to 3 hours, fully adjustable Operating Conditions Temperature 4 to 35° C Humidity 10 to 90% relative humidity Dimensions (D x W x H) 40 x 25 x 51.1 cm Weight (dry) 14 kg (30 lbs) Electrical connection 3-wire power cord. Power requirements 115 V AC models 90-140 V AC, 50/60 Hz, 6 Amps 230 V AC models 200-264 V AC, 50/60 Hz, 4 Amps Fuses required Type T, 250 V AC 115 V AC models 8 Amp; 5 x 20 mm; slo blo (2) 230 V AC models 6.3 Amp; 5 x 20 mm; slo blo (2) Regulatory Certified to I.E.C. 1010/Low Voltage Directive 73/23/EEC Certified to E.M.C. Directive 89/336/EEC (220 V only)/ EN55014 Emissions EN50082-2 Immunity Specifications 10

The HydroTech Vacuum Pump is warranted for 1 year against defects in materials and workmanship. If any defects should occur during this warranty period, Bio-Rad Laboratories will replace the defective parts with- out charge. However, the following defects are specifically excluded: 1. Defects caused by improper operation. 2. Repair or modification done by anyone other than Bio-Rad Laboratories or their authorized agent. 3. Use with fittings or other spare parts not specified by Bio-Rad Laboratories. 4. Damage caused by deliberate or accidental misuse. 5. Damage caused by disaster. 6. Tubing and fittings. For inquiry or request for repair service, contact your local Bio-Rad office. Warranty and Ordering Information 11

Warranty Period: ______________________________________________________________ ORDERING INFORMATION Catalog Number Description Gel Dryer and Vacuum Systems 165-1789 HydroTech Gel Drying System, 100/120 V, includes Model 583 Gel Dryer, HydroTech Vacuum Pump, tubing and connectors. 165-1790 HydroTech Gel Drying System, 220/240 V, includes Model 583 Gel Dryer, HydroTech Vacuum Pump, tubing and connectors. 165-1781 HydroTech Vacuum Pump, 100/120 V, includes pump, quick disconnect fittings for 1/4-inch and 3/8-inch ID vacuum tubing, vacuum tubing, drain tubing, and instructions. 165-1782 HydroTech Vacuum Pump, 220/240 V, includes pump, quick disconnect fittings for 1/4-inch and 3/8-inch ID vacuum tubing, vacuum tubing, drain tubing, and instructions. 165-1788 HydroTech Vacuum Gauge, includes fittings to connect to the HydroTech vacuum line. Warranty and Ordering Information 12

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