HARVARD CMA 7 Operation & User’s Manual

TECHNICAL INFORMATION Membrane Material Polyethersulfone (PES) Molecular Cut-Off 55,000 Daltons Outer Diameter 0.26 mm Length 1 and 2 mm Probe Shaft Material Stainless-steel Diameter 0.40 mm Length 7 mm Internal Volume Inlet Volume 0.06 µL Outlet Volume 0.3 µL 200 mm Inlet Tubing (blue) 3.5 µL 200 mm Outlet Tubing (transparent) 3.5 µL Instructions for CMA 7 55 kDa Microdialysis Probe 1. Fill a microsyringe with perfusion fluid and mount it in the CMA Syringe Pump. The Perfusion Fluid must be clean, at room temperature and preferably degassed. 2. Run the pump to make sure that liquid leaves the tip of the syringe cannula. 3. Connect the desired length of tubing to the inlet and outlet of the probe. Short cannula = inlet, long cannula = outlet. Tubing Adaptors and FEP Tubing should be used for all connections. To facilitate the handling of Tubing Adaptors, they should be pre-soaked in ethanol for a minimum of 10 minutes. 4. Mount the microdialysis probe CMA 7 & 8 Probe Clip on the CMA/130 in vivo Stand. Put the microdialysis probe in a vial filled with perfusion fluid. 5. Connect the inlet tubing of the microdialysis probe to the syringe cannula, by sliding the Tubing Adapter over the cannula. Wait for 10 min. The Tubing Adapter must be dry before flushing. 6. Flush the probe with 8-10 µL/min in the Perfusion Fluid for 4-5 min to wash out air. While flushing, “tap” with a scissor on the probe clip (not the probe) to remove air bubbles. The vibrations from the probe clip will in most cases remove the air bubble. If possible, check for air bubbles under a stereomicroscope. If the air bubble is not gone, the flushing and “tap- ping” must be repeated. The membrane is light blue when wetted, air bubbles occur as whiter spots. When flushing the membrane it may appear to be “sweating” which is due to ultrafiltration of fluid through the membrane. 7. Set the pump to the required perfusion flow (usually 1-5 µL/min) and check for leaks. The microdialysis probe is now ready for use. 8. When changing sample vials, remember to consider the internal volume in the system (see TECHNICAL INFORMATION). This causes a delay that must be calculated when using low perfusion rates and short sampling times. 9. After the experiment, put the microdialysis probe in a vial filled with deionized water. Perfuse with deionized water to prevent salt crystal formation. The probe can be stored in deionized water.

a period of two years from the manufacturing date if stored in the original package. Claims should be forwarded without delay to CMA Microdialysis or to your local distributor. CMA Microdialysis AB Head Office, Sweden Torshamnsgatan 30A, SE-164 40 Kista, Sweden Tel: +46 8 470 10 00 E-mail: [email protected] • www.microdialysis.com Harvard Apparatus 84 October Hill Road Holliston, MA 01746 USA Phone Orders: 800-232-2380 • Fax: 508-429-5732 E-mail: [email protected] • www.harvardapparatus.com The CMA 7 Microdialysis Probe is not intended for use in humans. It is only suitable for laboratory research in animals. CMA Micr�odialysis only guarantees single usage of CMA 7 Microdialysis Probes. ORDER INFORMATION Ref No. CMA 8012411 CMA 8012412 CMA 7 55kDa Microdialysis Probe, 1 mm, 3/pkg* CMA 7 55kDa Microdialysis Probe, 2 mm, 3/pkg* *Metal Free and β –Irradiated Probes are available as Custom Probes CMA 7 Guide Cannula, 3/pkg CMA P000137 CMA 7 Guide Cannula, 30/pkg CMA P000138 CMA 7 Guide Metal Free, 3/pkg CMA 8010773 CMA 7 Guide β –Irradiated, 3/pkg CMA 8010683 CMA 7 Guide β –Irradiated, 30/pkg CMA 8010684 Tubing Adapter, 10/pkg CMA 3409500 FEP Tubing 1 m, 1/pkg CMA 3409501 FEP Tubing 1 m, 10/pkg CMA 8409501 Tubing Connector, 3/pkg CMA P000113 CMA 7 & 8 Probe Clip CMA P000136 OPTIONAL ACCESSORIES Ref No. CMA 4004 Syringe Pump CMA 400400 CMA 402 Microdialysis Pump with Accessory Kit CMA 8003100 CMA 402 Microdialysis Pump CMA 8003110 CMA 110 Liquid Switch CMA 8308200 CMA 130 In Vitro Stand with CMA 7 clips CMA 8309104 CMA 142 Microfraction Collector 230V CMA 8381142 CMA 142 Microfraction Collector 115V CMA 8381143 CMA 470 Refrigerated Fraction Collector CMA 8002770 Microsyringes 1 mL CMA 8309020 Microsyringes 2.5 mL CMA 8309021 For other probes and microdialysis accessories please call your local CMA Microdialysis dealer. 8012410A