Pyxis SP-910 Operation Manual

Operation Manual Rev. A Firmware version 1.0.170  Pyxis Lab, Inc 2016 All Rights Reserved

1.1 Pyxis Major Features...............................................................................6 1.2 Unpackaging the Instrument.................................................................. 7 1.3 Standard Accessories..............................................................................8 1.4 Optional Accessories...............................................................................8 1.5 Sample Vial Compartment......................................................................8 1.6 Light Shield Cover................................................................................... 8 2 Start Pyxis SP-910.........................................................................................10 2.1 Battery Installation............................................................................... 10 2.2 Description of the Navigational Control Pad........................................10 2.3 Turning On Pyxis SP-910....................................................................... 11 2.4 Main Page............................................................................................. 11 2.5 Turning Off Pyxis SP-910.......................................................................12 2.6 Pyxis SP-910 Auto Power Off................................................................ 12 2.7 Auto LCD Power Saving.........................................................................12 3 PTSA Measurement..................................................................................... 13 3.1 PTSA Measurement.............................................................................. 13 3.2 PTSA calibration....................................................................................13 4 Fluorescein Measurement...........................................................................14 4.1 Fluorescein Measurement............................................................... 14 4.2 Fluorescein calibration......................................................................15 5 Colorimetric Measurement......................................................................... 17 5.1 Supported Methods..............................................................................17 5.2 Select a Method....................................................................................19 5.3 Single Timing Step Method...................................................................19 5.4 Single-Vial Procedure............................................................................20 5.5 Two-Vial Methods.................................................................................20 5.6 Multiple Timing Steps........................................................................... 21

5.8 Method Setup and Calibration............................................................. 23 6 Turbidity Measurement............................................................................ 25 6.1 Operation..............................................................................................25 6.2 Turbidity Calibration............................................................................. 25 7 Absorbance Measurement...................................................................... 27 8 Bluetooth Interface................................................................................... 28 8.1 Install Software.....................................................................................28 8.2 Open SP-910 Bluetooth Interface.........................................................28 8.3 Scan and Connect................................................................................. 28 8.4 Device Configuration............................................................................ 29 Setup Device Name.................................................................................29 Setup Product......................................................................................... 29 Add User Defined Colorimetric Methods............................................... 29 9 Maintenance.............................................................................................. 30 10 Troubleshooting...................................................................................... 32 11 Appendix A.............................................................................................. 33

Figure 2 Open and Close the Light Shield Cover...............................................9 Figure 3 Replace Batteries.............................................................................. 10 Figure 4 Main Menu........................................................................................11 Figure 5 PTSA Measurement.......................................................................... 13 Figure 6 PTSA Calibration................................................................................14 Figure 7 Fluorescein Measurement................................................................15 Figure 8 Fluorescein Calibration..................................................................... 15 Figure 9 Method Selection..............................................................................19 Figure 10 Single Timer Method...................................................................... 20 Figure 11 Concentration as a Function of Time..............................................20 Figure 12 Multiple Timer Method.................................................................. 22 Figure 13 Method Form Selection..................................................................24 Figure 14 Method Unit Selection....................................................................24 Figure 15 Slope Calibration.............................................................................24 Figure 16 Turbidity Calibration-1....................................................................26 Figure 17 Turbidity Calibration-2....................................................................26 Figure 18 Absorbance Measurement............................................................. 27 Figure 19 Nebula Scans Bluetooth Device......................................................28 Figure 20 Change Device Name......................................................................29 Tables Table 1 Feature Groups on Main Menu................................................................11 Table 2 List of Supported Colorimetric Methods..................................................17 Table 3 Wavelength of each channel....................................................................27 Table 4 Contact Information................................................................................. 32

Hach ® is a registered trademark of the Hach Company, Loveland, CO USA Confidentiality The information contained in this manual may be confidential and proprietary and is the property of Pyxis Lab. Information disclosed herein shall not be used to manufacture, construct, orotherwise reproduce the goods disclosed herein. The information disclosed herein shall not be disclosed toothers or made public in any manner without the express written consent of Pyxis Lab. Standard Limited Warranty PyxisLab warrants its products for defects in materials and workmanship. PyxisLab will, at its option, repair or replace instrument components that prove to be defective with new or remanufactured components (i.e., equivalent to new). The warranty set forth isexclusive and no other warranty, whether written or oral, is expressed or implied. Warranty Term The Pyxis warranty term is thirteen (13) months ex-works. In no event shall the standard limited warranty coverage extend beyond thirteen (13) months from original shipment date. Warranty Service Damaged or dysfunctional instruments may be returned to Pyxis for repair or replacement. In some instances, replacement instruments may be available for short duration loan or lease. Pyxis warrants that any labor services provided shall conform to the reasonable standards of technical competency and performance effective at the time of delivery. All service interventions are to be reviewed and authorized as correct and complete at the completion of the service by a customer representative, or designate. Pyxis warrants these services for 30 days after the authorization and will correct any qualifying deficiency in labor provided that the labor service deficiency is exactly related to the originating event. No other remedy, other than the provision of labor services, may be applicable. Repair components (parts and materials), but not consumables, provided in the course of a repair, or purchased individually, are warranted for 90 days ex-works for materials and workmanship. In no event will the incorporation of a warranted repair component into an instrument extend the whole instrument’s warranty beyond its original term. Shipping A Repair Authorization Number (RA) must be obtained from the Technical Support (service@pyxis- lab.com) before any product can be returned to the factory. Pyxis will pay freight chargesto ship replacement or repaired products to the customer. The customer shall pay freight charges for returning products to Pyxis. Any product returned to the factory without an RA number will be returned to the customer.

Specification  Colorimeter Wavelength: 365, 420, 455, 525, 560, 570, and 630nm  Turbidity Excitation Wavelength: White LED Infrared LED/90 degree scattering  Fluorescence Excitation Wavelength: 365 nm LED 460nmLED  Fluorescence Emission Wavelength: 410 nm 520nm  Wavelength Accuracy: ±1 nm  Absorbance Reproducibility: 0.005 au in the range of 0 to 1.0 au (3sigma)  Absorbance Linearity Range: 0 to 1.0 au  PTSA Fluorescence Reproducibility: 1 ppb PTSA (3 sigma)  PTSA Fluorescence Detection Limit: 1 ppb  Fluorescein Fluorescence Reproducibility:0.1ppb  Fluorescein Fluorescence Detection Limit:0.1ppb  Turbidity Reproducibility: 1 NTU (3 sigma)  Turbidity Detection Limit: 1 NTU  Battery: 4 AA alkaline  Typical Battery Life: 2 months  Display: Graphical LCD 192x128 pixels, visible under direct sunlight  Instrument Dimension: L 265mm W 88mm H 62mm  Instrument Weight: 600 g without batteries  Storage Temperature Range: 0 to 140°F (-18 to 60°C)  Operation Temperature Range: 40 to 120 °F (4 to 49°C)  Humidity: 85% at 106 °F (41 °C)  Environmental: IP67, dustproof and waterproof Note: 1. Specifications are subject to change without notice with Pyxis’ continuous development. 1.1 Pyxis Major Features The Pyxis SP-910 analyzer shown in Figure 1 is a combination of photometer and fluorometer. It provides colorimetric measurements at 6 LED wavelengths, fluorometric measurement of fluorescent tracer PTSA and fluorescein, and nephelometric turbidity measurement using white LED and infrared LED as the excitation sources. Pyxis SP-

water treatment and other water testing in the laboratory or in the field, such as chlorine, phosphate, iron, and copper. Main features include:  Pyxis SP-910 is pre-calibrated for measuring PTSA (pyrenetetrasulfonic acid) in the range of 0 to 300 ppb. The fluorescence PTSA measurement is automatically compensated for sample color and turbidity interference.  Pyxis SP-910 is pre-calibrated for measuring fluorescein in the range of 0 to 20 ppb.  Pyxis SP-910 is pre-calibrated for measuring turbidity in the range of 0 to 500 NTU.  Automatically select the primary wavelength according to the method selected and switches to the secondary wavelength to extend the primary measurement range. Navigational Control Pad LCD Sample Vial Compartment Light Shield Cover Sample Vial Figure 1 Insert the Sample Vial  Display a concentration-time profile curve during the last time period in a colorimetric measurement. The user can terminate the timing process and take a reading if the displayed concentration reaches a plateau before completing the predefined time period.  The user can update the calibration parameter of any pre-calibrated colorimetric method by testing a standard solution first and then following a setup procedure to update the calibration parameters. 1.2 Unpackaging the Instrument Remove the instrument and accessories from the shipping container and inspect each item for any damage that may have occurred during shipping. Verify that all items listed on the packing slip are included. If any items are missing or damaged, please contact Pyxis Customer Service at [email protected].

 Sample Vials – two 10 ml, round, 0.78 inch (20 mm) pathlength, glass vials, which can be used for all measurements including turbidity and fluorescence measurements.  16 mm adapter  4 AA alkaline batteries  Instrument Manual, also available from www.pyxis-lab.com 1.4 Optional Accessories  Silica desiccant gel pack  100 ppb PTSA standard in a 500 ml brown plastic bottle  20 ppb fluorescein standard in a 500 ml brown plastic bottle 1.5 Sample Vial Compartment The sample vial compartment is shown in Figure 1, along with a 10-ml sample vial. When the sample vial is inserted into the sample vial compartment, the triangular mark on the sample vial should be aligned approximately with the 6 o’clock position of the sample vial compartment or any position consistently. The sample vial compartment should be kept clean. A small amount foreign material could significantly affect turbidity and fluorescence measurement results. Use a soft cloth or lint free paper tissue to clean sample vial compartment periodically. Remove debris, scale, and deposit promptly. 1.6 Light Shield Cover The light shield cover is shown in Figure 2. The light shield cover can be conveniently slid between the open and closed positions. The light shield cover is held firmly at the rest positions by permanent magnets. The light shield cover should be in the closed position during storage, transportation, and measurements, especially during the turbidity and fluorescence measurements. When turned on, Pyxis SP-910 carries out self-diagnosis including checking the performance of a variety of optical devices. The light shield door shall be at the closed position to shield interference from ambient light during self-diagnosis. Care should be taken to avoid water or debris being trapped in the track of the light shield door.

Warning Magnetic sensitive devices, including but not limited to, credit cards, watched, hard disks, should be keep at a distance of at least 2 inches from the Light Shield Door to avoid possible damage and/or loss of information recorded.

2.1 Battery Installation Pyxis SP-910 is powered by four AA-size alkaline batteries. Do not use rechargeable nickel cadmium (NiCad) batteries or any AA-size lithium batteries. A set of batteries typically lasts for two months. When the batteries capacity is low, Pyxis SP-910 will prompt a LOW BATTERY warning. Replace all four batteries to resume operation of Pyxis SP-910 after the battery warning. The Pyxis SP-910 battery compartment, shown in Figure 3, is on the back side of the instrument. Insert a small pad underneath the screen area to make the back surface level when the instrument is turned upside down. Install batteries as followings: 1. Remove the battery compartment cover by loosening four screws. 2. Insert four batteries into the battery holder as shown in Figure 3. Make sure the positive battery polarity marker (+) is aligned with the positive marker (+) on the battery holder. 3. Replace the battery compartment cover, making sure that the sealing O-ring is lying flat on the battery holder and tighten the four screws. Figure 3 Replace Batteries 2.2 Description of the Navigational Control Pad The Pyxis SP-910 navigational control pad consists of five keys as shown in Figure 1. The left, right, up, and down keys are navigational keys that are used to select an icon, a button, or other items in various pages. The center key is the OK key. Press the OK key on a selected item to launch the action associated with the selected item. The OK

keyboard. 2.3 Turning On Pyxis SP-910 To turn on SP-910, press and hold on the OK key for 3 seconds, and release the OK key when the LCD is lit. After new batteries installation,Pyxis SP-910 will be automatically turned on, and its LCD backlight will be on. You can navigate the main page menu and launch an operation by pressing on an icon. If battery voltage is too low for the instrument to work properly, Pyxis SP-910will show a low battery warning message for 5 seconds and turn off automatically. If this happens, replace all four batteries. 2.4 Main Page Pyxis SP-910provides intuitive icon menu-assisted user operations. On the main page, eight major feature groups are illustrated as below: Figure 4 Main Menu A brief description of each feature group is given in Table 1.Detailed operation instructions can be found in the following chapters. Table 1 Feature Groups on Main Menu No. Title Description 1 M-P PTSA measurement 2 M-F Fluorescein measurement 3 COLOR Colorimetric measurement methods 4 TURB Turbidity measurement 5 Power Turn off SP-910 6 ABSB Absorbance measurements 7 CAL Calibration routines 8 SYS System information

Turn Pyxis SP-910 off by navigating to Power icon and press the OK key. 2.6 Pyxis SP-910 Auto Power Off Pyxis SP-910 automatically turns itself off with no-key activity, except for during a measurement.The auto power off time can be set. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data. 2.7 Auto LCD Power Saving During a colorimetric method measurement, Pyxis SP-910 automatically turns LCD backlight off with no-key activity. The auto LCD power time can be set.Pressing any key will turn on the LCD backlight. Under normal ambient lighting condition, icons and other contents shown on Pyxis SP-910LCD are readable without backlight being on.

3.1 PTSA Measurement 1. Fill the 10 ml sample vial with the test solution and tightly cap the sample vial. 2. Place the sample vial into the sample vial compartment and slide the light shield cover to the closed position. 3. Press the M-P on the main page, then press the ok button,Pyxis SP-910 will start to measure the PTSA concentration in the sample. 4. PyxisSP-910 will display the PTSA concentration in ppb as PTSA. Figure 5 PTSA Measurement During the fluorescence measurement to determine the PTSA concentration, Pyxis SP-910 checks the sample turbidity. If the sample turbidity value detected is greater than 40 NTU, Pyxis SP-910 will display a warning.For best results, the sample should be filtered if turbidity exceeds 40 NTU. Sample color causes a lower PTSA concentration to be measured. Pyxis SP-910 automatically compensates for sample color. If the sample color is too intense, Pyxis SP-910 will display a warning. For best results, ensure that the sample vial is clean. Wipe off water on the outside wall of the sample vial using a lint-free tissue paper. Fill the sample vial to the 10 ml mark. If the sample contains air bubbles, tap the sample vial gently to remove the bubbles before placing the sample vial to sample vial compartment. 3.2 PTSA calibration Deionized water (DI) as the blank calibration solution and the 100 ppb PTSA calibration standard solution are needed. 1. Press the CAL on the main page, then choose the M-P and press the OK key to launch the PTSA calibration page. 2. Follow the message prompts, insert the DI blank into the sample vial compartment and press the OK key.

compartment and press the OK key. 4. Press the OK key to return to the main page. Figure 6 PTSA Calibration If calibration fails, the followings should be checked:  The DI blank is being contaminated.  The 100 ppb PTSA standard solution is decayed or being contaminated.  The light shield cover is not in the closing position.  The sample vial compartment is blocked with debris, water, or other materials. The 100 ppb standard solution shall be stored in a brown or black opaque bottle. Exposing the PTSA standard to light will cause the standard losing the PTSA concentration. Many substances, such as quaternary amine cause a negative interference. Many other substances such laundry detergents that contain optical brightener will cause a significant positive interference. 4 Fluorescein Measurement 4.1 Fluorescein Measurement 1. Fill the 10 ml sample vial with the test solution and tightly cap the sample vial. 2. Place the sample vial into the sample vial compartment and slide the light shieldcoverto the closed position. 3. Press the M-F on the main page, then press the ok button,Pyxis SP-910 will start to measure the fluorescein concentration in the sample. 4. Pyxis SP-910 will display the fluorescein concentration in ppb as fluorescein.

For best results, ensure that the sample vial is clean. Wipe off water on the outside wall of the sample vial using a lint-free tissue paper. Fill the sample vial to the 10 ml mark. If the sample contains air bubbles, tap the sample vial gently to remove the bubbles before placing the sample vial to sample vial compartment. 4.2 Fluorescein calibration Deionized water (DI) as the blank calibration solution and the 20 ppb fluorescein calibration standard solution are needed. 1. Press the CAL on the main page, then choose the M-F and press the OK key to launch the fluorescein calibration page. 2. Follow the message prompts, insert the DI blank into the sample vial compartment and press the OK key. 3. Follow the message prompts, and insert the 20 ppb standard into the sample vial compartment and press the OK key. 4. Press the OK key to return to the main page. Figure 8 Fluorescein Calibration If calibration fails, the followings should be checked:  The DI blank is being contaminated.  The 20 ppb fluorescein standard solution is decayed or being contaminated.  The light shield cover is not in the closing position.

The 20 ppb standard solution shall be stored in a brown or black opaque bottle. Exposing the fluorescein standard to light will cause the standard losing the fluorescein concentration. Many substances, such as quaternary amine cause a negative interference. Many other substances such laundry detergents that contain optical brightener will cause a significant positive interference.

5.1 Supported Methods A wide range of colorimetric methods is supported by the Pyxis SP-910 analyzer and the number of them keeps increasing with continuous development of Pyxis. See corresponding Hach® methods in Appendix A. Table 2 List of Supported Colorimetric Methods Abbreviated Method Name Method Name Description Range CL-F F-Chlorine Free chlorine, DPD method 2.2 ppm CL-T T-Chlorine Total chlorine, DPD method 2.2 ppm CL2H CL2High High range, DPD method 6.0ppm CuBi Cu_Bicinch Bicinchoninate, EPA approved for reporting wastewater analysis 5.0 ppm DEHA DEHA Method for N,N- diethylhydroxylamine and other oxygen scavengers 0.5 ppm Ca Ca Calmagite method for calcium 4.00 ppm as CaCO3 Mg Mg Calmagite method for magnesium 4.00 ppm as CaCO3 FePh Fe_phenanth Total iron using 1,10- phenanthroline, USEPA approved for reporting wasterwater analysis 3.00 ppm FeZi FeZine FerroZine method 1.300 ppm FeTp FeTptz Total iron using TPTZ 1.80 ppm MoHR Mo_HighRange High range molybdate 40.0 ppm MoLR Mo_LowRange Low range molybdate using ternary complex method 3.0 ppm NO2H NO2H High range nitrite, ferrous sulfate method 150 ppm as NO2 NO2L NO2L Low range nitrite, diazotization method, USEPA approved for reporting wastewater and drinking water analysis 0.350 ppm as NO2 PMoV OPO4-MoV Reactive phosphate using molybdovanadate method 30.0 ppm as PO4 OPO4 OPO4 Reactive phosphate using ascorbic acid molybdenum blue method, USEPA accepted for wastewater analysis 2.5 ppm as PO4 OrgP Phosphonate UV digestion and ascorbic acid reduction molybdenum blue 7.1 ppm as PBTC

acid reduction method 30.0 ppm ClO2 ClO2-DPD DPD method, USEPA accepted for reporting drinking water analysis 5.00 ppm ClO2D ClO2Direct Direct method for chlorine dioxide 35.0 ppm SiHR SiHR High range silica 75.0 ppm as SiO2 SiLR SiLR Low range silica 1.60 ppm as SiO2 AZOL Azole UV digestion for tolyltriazole and benzotriazole 16.0 ppm SO4 SO4 Barium sulfate turbdimetric method 70.0 ppm POLY Polymer Turbidimetric method for anionic polymeric dispersant 13.0 ppm as PAA FeMo FeMo Total iron method for water containing molybdate 1.80 ppm Cr6 Cr6 1,5-Diphenylcarbohydrazide method for chromium hexavalent,USEPA accepted for wastewater analyses 0.6ppm CrT CrTot Alkaline hypobromite Oxidation method for chromium total 0.6ppm NH3S NH3Sal Salicylate method for nitrogen,ammonia 0.5ppm NH2C NH2CL Indophenol method for chloramine mono 3.0ppm N2H4 N2H4 p-Dimethylaminobenzaldehyde method for hydrazine 0.5ppm MnL MnLow Low range manganese 0.7ppm MnH MnHigh High range manganese 20.0ppm BLCH Bleach Direct Method measuring sodium hypochlorite concentration AL Alumi Aluminon method for aluminum 0.8ppm F Floride SPADNS method for fluoride 2.0ppm CuL CuPorp Porphyrin Method for copper 0.2ppm Zn Zinc Zincon method for zinc,USEPA approved for wastewater analysis 3.0ppm S2- Sulfide Methylene blue method for sulfide,USEPA accepted for reporting wastewater analysis 0.7ppm

cyanide 0.2ppm NO3M NO3M Middle range nitrate 5ppm as N NO3H NO3H High range nitrate 30ppm as N Ni Ni PAN method for nickel 1ppm CYAN CYAN Turbidimetric method for cyanuric acid 55ppm pH pH Phenol red method for pH 8.5 5.2 Select a Method Move the icon focus to the method icon Mthd using the navigational (left, right, up, or down) keys. Press OK on the icon to launch the first method selection page. The methods shown on the top row of the page are the most frequently selected methods. Figure 9 Method Selection The followings are the operations associated with this page: 1. Use the navigational keys and the OK key to select and launch a method. 2. Press the arrow icon at the end of the favorite methods row to return to the main page.Press the arrow icon at the lower right corner of the page to display the second method selection page if the device is loaded with more than 23 methods. Note: Methods shown in the method selection pages include Hach© equivalent methods and Pyxis specific advanced methods. The table in Appendix A provides a brief description of Pyxis method names and their corresponding Hach® program number. Hach® reagents for 10 ml sample can be used for the test. 5.3 Single Timing Step Method Most of colorimetric methods have only one timing step. As an example, in the DPD freechlorine method, it takes three minutes for the DPD powder reagent to completely react with chlorine in the water sample. The DPD free chlorine method has a single

timing step. Figure 10 Single Timer Method 5.4 Single-Vial Procedure 1. Place the sample vial filled with the water sample in the Pyxis SP-910 sample vial compartment and press the ZERO button. Pyxis SP-910will display the page shown in Figure 11. 2. Take the sample vial out and add the reagent to the sample vial. 3. Place sample vial back into the sample vial compartment and press the timer button TIMER. Pyxis SP-910 will start to monitor the reaction between the reagent and the species you want to measure in the water sample. The concentration is shown in the chart as a function of time (Figure11). 4. When the timer reaches the preset time and the reaction is complete, the value of concentration will be shown on the top right corner of the page. 5. The rate of the reaction is often faster than the standard pre-set time, which will become apparent from the concentration-time plot. You can press the STOP button to stop the timer and terminate the timing step. The last read concentration value will be displayed on the top right corner of the page after you terminate the timing step. Figure 11 Concentration as a Function of Time 5.5 Two-Vial Methods

identical vials. One vial is being used to zero the colorimeter, referred as to the prepared blank. A reagent is added to the other vial, referred as to the prepared sample. The absorbance value is determined from the prepared sample. If the method requires two or more reagents, the prepared blank could be the resulting solution after one or more reagents have been added to the sample. The following procedure is typical for two-vial methods: 1. Place the prepared blank into the Pyxis SP-910 sample vial compartment and press the ZERO button to zero the instrument. 2. Place the prepared sample into the Pyxis SP-910 sample vial compartment and press the TIMER button to start the method timer. 3. When the timing step is completed, the measured concentration will be displayed on the top of the page. The timing step could be terminated earlier by pressing STOP button. 4. Optionally, Pyxis SP-910can be re-zeroed using the prepared blank after the timing step is completed or terminated. The blank reading will be subtracted from the measured concentration value, and the displayed concentration value on the top-right corner will be updated. This step is optional. It is only necessary if the prepared blank changes its color during the timing period. 5. Optionally, the prepared sample vial can be put back and read again by pressing the READ button if the blank is re-zeroed after the timing step is completed or terminated. A new concentration value based on the last absorbance value measured will be calculated and displayed. 5.6 Multiple Timing Steps Some colorimetric methods have two or three timing steps. Pyxis SP-910 shows a count-down timer for the timing steps before the last timing step (Figure 12). During these timing steps, one or more reagents are added to the sample, or operations such as swirling the vial to mix the reagent and the sample are being performed. These methods usually use one vial for the prepared blank and the other for the prepared sample.

In order to show the concentration-time curve as shown in Figure 9during the last timing step, Pyxis SP-910must be zeroed using the prepared blank before the last timing step. Thus, the last timer button will not be selectable until Pyxis SP-910 has been zeroed using the prepared blank. Multi-timing step Hach® methods require zeroing the colorimeter using the prepared blank after the last timing step is completed. Pyxis SP-910can optionally be re-zeroed using the prepared blank after the last timing step. The blank value measured will be subtracted from the concentration value measured at the end of the last timing step. Optionally, the READ button could be pressed to read the prepare sample again. The following procedure is typical for methods having two-timing steps: 1. Press the TIMER1 button to start the first timer. Complete the necessary operations to prepare the blank and the sample. 2. Place the prepared blank into the Pyxis SP-910 sample vial compartment and press the ZERO button. 3. Place the prepared sample into the Pyxis SP-910 sample vial compartment and press the TIMER2 button to start the second timer. Pyxis SP-910 will display the measured concentration as a function of time as shown in Figure 9. 4. When the timing step is completed, the measured concentration will be displayed on the top right of the screen. The timing step could be terminated earlier by pressing STOP button. 5. Optionally, Pyxis SP-910can be re-zeroed using the prepared blank after the timing step is completed or terminated. The blank reading will be subtracted from the measured concentration value, and the displayed concentration value on the top-right corner will be updated. This step is optional. It is only necessary if the prepared blank changes its color during the timing period. 5.7 Advanced Methods Pyxis SP-910provides 6 LED wavelengths and can measure absorbance values at multiple LED wavelengths. Consequently, Pyxis SP-910can provide many predefined advanced methods that traditionally require complex and often expensive lab testing procedures. Low range, direct reading chlorine dioxide, 0 to 35.0 ppm The maximum absorption bank of aqueous chlorine dioxide is around 360nm. Pyxis SP-910has a 365nm UV LED and can be used to directly measure chlorine dioxide. It offers a much lower detection limit (0.2 ppm) than direct methods available from other portable colorimeters having only light sources in the visible range.

measure chlorine dioxide: 1. Place a vial filled with deionized water into the vial compartment and press the ZERO button to zero Pyxis SP-910. 2. Discard the deionized water and fill the same vial with the sample. Place the vial into the vial compartment and press READ button to read. The measured chlorine dioxide concentration will be displayed in the top of the method page. Turbdimetric Anionic Polymer Method 1. Add polymer reagent 1 to 10 ml sample and inverse the sample vial 5 times to mix the reagent with the sample. Place the sample via to the sample vial compartment. 2. Press on ZERO. 3. Add polymer reagent 2 and press on TMR1 to start the five minutes timer. 4. Gently inverse the sample via for 10 times and place the sample vial to the sample vial compartment. 5. Polymer concentration will be measured and displayed when the five minute timer is reached. The polymer concentration is shown as ppm PAA (polyacrylic acid) equivalent. 5.8 Method Setup and Calibration Press the SETUP button in the method result page to launch the method setup and calibration page. 5.8.1 Set up the method parameters Press the FORM button to select a concentration form from the list of forms that are available for this specific method (Figure 13). Press the UNIT button to select a concentration unit among the list of ppb, ppm, mg/L, and ug/L (Fihure 14).

Figure 14 Method Unit Selection 5.8.2 Slope Calibration If the method has been calibrated priorto shipping, there is no need to calibrate unless a calibration check indicates that the method needs a calibration. The following steps are used to calibration a method: 1. Use a calibration standard of known concentration. Follow the steps required by the method and note the value reported by SP-910. 2. If the measured value differs from the known standard value,Press theCONFG button to launch the method configuration page. 3. Press the slope calibration button SlpCal. A numeric keyboard will be displayed. 4. Enter the concentration value and press the OK key on the enter key in the numeric keyboard to return to the configuration page. 5. Press the EXIT button. Press the OK key to accept the calibration or other key to cancel the calibration. Figure 15 Slope Calibration For best results, the concentration of the standard solution should be less than the maximum concentration for the method (table 2) and greater than the half of the maximum concentration. For example to calibrate total chlorine, the

ppm. The corresponding calibration parameters will be updated and saved in the memory as the working calibration parameter set. Note that this set of calibration parameters are not the same as the default set. You can use Default button to copy the default calibration parameters to the working set. 5.8.3 Reagent Blank Calibration Some methods have a non-zero intercept value in the calibration equation. For these methods, a proper non-zero intercept value is pre-loaded in SP-910prior to shipping. The following steps are used to carry out a reagent blank calibration: 1. Follow the normal steps to carry out a measurement on a deionized water sample. 2. Press the CONFG button to launch the method configuration page. 3. Press the reagent blank calibration button BLKC 4. Press the OK key to save when exiting from the configuration page or press other keys to cancel. 5.8.4 Resume to Default Calibration Parameters Pressing the Default button will copy the default calibration intercept and slope to the working intercept and slope, respectively. If the default calibration parameters were created prior to shipping, this button action is to restore the working calibration parameters to the original factory loaded calibration parameters. 6 Turbidity Measurement 6.1 Operation Follow the following steps to measure turbidity: 1. Fill the 10 ml sample vial to above the 10 ml mark. 2. Insert the sample vial to the sample vial compartment. 3. Slide the light shield cover to the closed position. 4. Press the Turb on the main page, then press the OK key, Pyxis SP-910 will start to measure the turbidity in the sample. 6.2 Turbidity Calibration 1. Fill the 10 ml sample vial to above 10 ml mark with the deionized water. 2. Insert the sample vial to the sample vial compartment.

4. Press the CAL on the main page, then choose the Turb calibration and press the ok button to launch the Turb calibration page.(Figure 16) 5. Press the OK key to measure the deionized water 6. Fill the 10 ml sample vial to above 10 ml mark with the 100 NTU formazin standard. Insert the sample vial to the sample vial compartment. 7. Press the OK key to measure the 100 NTU standard. Low range turbidity calibration is successful 8. Press the OK key to continue high range turbidity calibration. If high range turbidity calibration not required,press any keys to exit. (Figure 17) 9. Fill the 10 ml sample vial to above 10 ml mark with the 400 NTU formazin standard. Insert the sample vial to the sample vial compartment. 10. Press the OK key to measure the 400 NTU standard. High range turbidity calibration is successful, 11. Press any keys to exit. Figure 16 Turbidity Calibration-1 Figure 17 Turbidity Calibration-2

1. Press the ABS to launch the absorbance measurement page. 2. Place a vial filled with the blank sample in the sample vial compartment. Press the ZERO button to zero the method. 3. Place a vial filled with the sample in the sample vial compartment. Press the READ button to read absorbance.The absorbance values of first 6 wavelengths (Table 3) will be shown. Press the READ button again to show the absorbance values of the last three wavelengths. Figure 18 Absorbance Measurement Press EXIT to return to the main page. Timing function for absorbance measurement may not be available for some models. Table 3 Wavelength of each channel Channel Wavelength (nm) 1 560 2 570 3 470 4 not used 5 455 6 525 7 365 8 630 9 420 Note that the absorbance values measured with Pyxis SP-910 is generally smaller than those measured with a spectrophotometer equipped with a monochromatic light source or detector. The Pyxis SP-910 absorbance values should, however, linearly correlate with the

system, Pyxis SP-910 absorbance follows Lambert-Beer law. 8 Bluetooth Interface SP-910 equipped with a Bluetooth interface, which allows a user to connect to SP-910 with a computer or a smartphone to do the following tasks:  Configure device  Add user defined colorimetric methods  Upgrade device firmware Also with the Bluetooth interface, user can calibrate an inline fluorometer directly from SP-910 in the field. Below sections describe how to connect and communicate with your SP-910 via a computer and Pyxis Nebula software. 8.1 Install Software Download Nebula software by clicking this link, unzip and install Nebula software, Bluetooth adapter driver will be installed as well. Plugin the Bluetooth adapter shipped along with your SP-910 device, open Nebula software. 8.2 Open SP-910 Bluetooth Interface Bluetooth function is normally switched off in order to reduce power consumption, to open Bluetooth interface, select SYS in the main menu and click BTLE in the next screen. 8.3 Scan and Connect Open Nebular software, select the right COM port, depends on hardware variance, actual COM port may not be same as below picture shows. Then click Scan button to scan Bluetooth enabled device, SP-910 device will appear in device list. Choose the SP-910 device by clicking device name, and click Connect button to connect to it. Figure 19 Nebula Scans Bluetooth Device

Setup Device Name When connected, the device information will be uploaded and displayed, only the device name is allowed to change as Fig 21 shows Figure 20 Change Device Name Setup Product Your product name product/PTSA ratio can be setup according to Fig 22 Figure 21 Setup Product Add User Defined Colorimetric Methods User can edit new method parameters in Nebula software and download to SP- 910via Bluetooth interface according to Fig 23. “Add”: add one empty method entry in user defined method list “Delete”: delete one method from user defined method list “Download”: send new method to SP-910 “Upload”: read user defined methods from SP-910 “Load/Save methods” read/write methods to local file

9 Maintenance Use a soft cloth or lint free paper tissue to clean the sample vial compartment periodically. Remove debris, scale, and deposit promptly. Although Pyxis SP-910 is protected from water damage, it is a good practice to avoid water entering the sample vial compartment and becoming trapping underneath the navigational control pad. Deposits left behind when the water is evaporated could affect Pyxis performance. Pyxis SP-910should be stored in the temperature range of 0 to 140°F (-18 to 60°C) and relative humidity less than 85% at 106 °F (41 °C). Do not leave the Pyxis SP-910 in a parked vehicle. The temperature inside a parked vehicle can reach above 150 °F in summer and -20 °F in winter. Exposing the Pyxis SP-910 to extreme temperature or humidity will cause a gradual decay in performance of fluorescence measurements and require more frequent calibrations. During storage and transportation, do not leave a sample vial in the sample vial compartment. Close the lid of the sample vial compartment during storage and transportation.

BATTERY voltage. Remove batteries from Pyxis SP-910 battery compartment if Pyxis SP-910 is going to be placed in storage for a long period time. When the Pyxis SP-910 is shipped, a desiccant pack is included in the desiccant compartment underneath the cover of the battery compartment. It is recommended that a new desiccant pack is replaced each time the batteries are replaced.

operation. On screen prompts direct the user to take appropriate corrective actions in most cases. If an unspecific error occurs or Pyxis SP-910 cannot be turned on, reboot the instrument by taking a battery out of the battery holder and re-install the battery. If the Pyxis SP-910 has been idle for more than two months and cannot be turned on, replace all four batteries with four new AA alkaline batteries. A diagnostics page can be launched by press the SYS icon in the main page. The software version and its associated hash code can be found in the diagnosis page. Contact Pyxis professionals at [email protected] and provide with following information to ensure high quality technical support. Table 4 Contact Information Items Note Contact Name Phone Email Customer Name Product Number (P/N) Can be found on the product label on back of product Serial Number (S/N) Can be found on the product label on back of product Firmware version Can be found in diagnosis page Problem Description Capture warning message if applicable

Pyxis Method and Hach® Method Number (PRMP) Cross Reference Abbreviated Method Name Method Name Corresponding Hach © method Hach Methed Number CL-F F-Chlorine Chlorine, Free, DPD, PRMP 9 8021 CL-T T-Chlorine Chlorine, Totoal, DPD, PRMP 9 8167 Cl2H Cl2High High Range DPD Chlorine, No sample change needed 10070 CuBi Cu_Bicinch Copper, Bicinchoninate, PRMP 20 8506 DEHA DEHA DEHA, Iron Reduction Method for Oxygen Scavengers, PRMP 25 8140 Ca Ca Calcium: Calmagite Colorimetric Method, PRMP 29 8030 Mg Mg Magnesium: Calmagite Colorimetric Method, PRMP 30 8030 FePh Fe_phenanth Iron, 1,10 phenanthroline, PRMP 33 8008 FeZi FeZine Iron, FerroZine, PRMP 37 8147 FeTp FeTptz Iron, TPTZ, PRMP 39 8112 MoHR Mo_HighRange Molybdenum, High Range, Mercaptoacetic Acid, PRMP 44 8036 MoLR Mo_LowRange Molybdenum, Low Range, Ternary Complex, PRMP 47 8169 NO2H NO2H Nitrite, High Range, Ferrous Sulfate, PRMP 59 8153 NO2L NO2L Nitrite, Low Range, Diazotization, PRMP 60 8507 PMoV OPO4-MoV Phosphorus, Reactive, Molybdovanadate, GRMP 77 8114 OPO4 OPO4 Phosphorus, Reactive, Orthophosphate Ascorbic Acid, GRMP 79 8048 OrgP Phosphonate Phosphonates, Persulfate UV Oxidation, PRMP 80 8007 PAmi OPO4-Amino Phosphorus, Reactive, Amino Acid, GRMP 85 8178 ClO2 ClO2-DPD Chlorine Dioxide, DPD, PRMP 112 10126 ClO2D ClO2Direct Chlorine Dioxide, Direct Reading,PRMP7 8345 SiHR SiHR Silica, High Range, Silicomolybdate, PRGM 89 8185 SiLR SiLR Silica, Low Range, Heteropoly Blue, PRMP 90 8186 AZOL Azole Benzotriazole, UV Photolysis, PRMP 3 8079

8051 POLY Polymer Turbidimetric method for anionic polymers N/A FeMo FeMo Iron, for cooling water with molybdenum-based treatment, PRMP 38 8365 Cr6 Cr6 Hexavalent chromium, 1,5- Diphenylcarbohydrzaide Method, PRMP 13 8023 CrT CrTot ChromiumtotalAlkaline Hypobromite Oxidation Method, PRMP15 8024 NH3S NH3Sal Ammonia Salicylate Method, PRMP 64 8155 NH2C NH2Cl Indophenol Method for MonoChloramine, PRMP 110 10171 N2H4 N2H4 P-Dimethylaminobenzaldehyde Method for Hydrazine, PRMP 31 8141 MnL MnLow Low Range Manganese PAN Method, PRMP 43 8149 MnH MnHigh High Range Manganese, Periodate Oxidation Method, PRMP 41 8034 BLCH Bleach Direct Method measuring sodium hypochlorite concentration N/A Al Alumi Aluminon Method for Aluminum, PRMP 1 8012 F Floride SPADNS 2 Method for Fluoride, PRMP 27 8029 CuL CuPorp Porphyrin Method for Copper, PRMP 22 8143 Zn Zinc Zincon Method for Zinc, PRMP 97 8009 S2- Sulfide Methylene Blue Method for Sulfide, PRMP 93 8131 CN Cyanide Pyridine-Pyrazalone Method for Cyanide, PRMP 23 8027 NO3M NO3M Middle range nitrate,PRMP54 8171 NO3H NO3H High range nitrate,PRMP51 8039 Ni Ni PAN method for nickel,PRMP48 8150 CYAN CYAN Turbidimetric method for cyanuric acid ,PRMP 24 8139 pH pH Phenol red method for pH,PRMP 75 10076 Hach ® is a registered trademark of the Hach Company, Loveland, CO USA