AmScope 150 Series Operation & User’s Manual

Before Use............................................................................................. 3 Introduction.............................................................................................................3 Safety Precautions...................................................................................................4 Parts....................................................................................................... 5 M150........................................................................................................................5 Definition of Parts...................................................................................................6 Getting Started..................................................................................... 7 Assembly..................................................................................................................7 Operation.............................................................................................. 8 Setting Up................................................................................................................8 Focusing...................................................................................................................8 Adjusting The Diaphragm.......................................................................................8 Attaching a Camera / Changing Eyepieces.............................................................8 Setting the Stage’s Stop-Limit..................................................................................9 Maintenance/Precautions.....................................................................................10 Specifications......................................................................................11 149 Series Specifications........................................................................................11 Optional Accessories..............................................................................................12 Objectives...............................................................................................................13 Eyepieces................................................................................................................13 Technical Parameters........................................................................14 Electrical System....................................................................................................14 Technical Terms & Concepts.................................................................................15 Common Issues......................................................................................................16 Common Issues (Continued)................................................................................17

This manual is designed for the M150 series microscopse. Please be sure to take a few minutes to familiarize yourself with the features and functions of your new AmScope microscope. If you’d like more information on microscopes, parts, or accessories, please visit our website at: www.iScopeCorp.com We highly recommend you study this manual thoroughly before operating the microscope, and that yo ukeep it on ahnd for future reference. If you have additional questions or need assistance, please send us an email at: [email protected]

or abrupt movement during transporation. Do not shake the package. 2. Do not place the microscope in direct sunlight or in high heat. Keap it indoors in a dry and clean place with temperateures between 32-100 degrees F (0-40 degrees C), and in maximum relative humidity of 85%. 3. Avoid touching the lenses on the objectives and the eyepieces so that oil and dirt from your fingerprints do not obstruct your view. 4. Before turning the power on, make sure that the power supply voltage is consident with the voltage of your microscope.

Stage Illuminator Lens Fine Focusing Knob Disc Diaphragm (Disc Iris) Limit Stop Stage Clips M150 Coarse Focusing Knob

Base Illuminator Lens Directs the light source towards the slide Focusing Knob Used to bring the slide into the sight and focus Disc Iris Diaphragm Controls the amount of light that hits the slide from the base illuminator Dimmer Controls the amount of light that escapes from the base lens Limit Stop Knob Limits the upward movement of the mechanical stage in order to avoid damaging the slide and objective Nosepiece Houses the objective lenses Rotating Monocular Head Houses the eyepiece and optics of the microscope

side is labeled up. Remove the tape and open the container carefully so as to avoid dropping and damaging the optical items. Check carefully to ensure that all parts and accessories are intact. 2. Check the packing list to ensure that you’re received all items: - One Monocular Microscope Body & Head - Three Objectives (4x, 10x, 40x) - 10x Widefield Eyepieces - 16x Widefield Eyepieces (for -A models only) - 20x Widefield Eyepieces (for -B models only) - 25x Widefield Eyepieces (for -C models only) - One Dust Cover Note: LED models do not have a spare bulb, as the LED bulbs do not need replacing. 3. Remove the microscope body from the box and remove the plastic protective covering. The body of the micro- scope is composed of the base, the stage, the arm, and the nosepiece. 4. Screw the objectives into the microscope nosepiece from the lowest magnification to the highest, again avoid- ing touching the lenses. 5. Plug in the microscope and turn it on. If no light emerges from the light source, adjust the dimmer knob on the side of the base.

in place with the metal slide holders (clips) of the mechanical stage. 2. Center the specimen over the stage opening, lining it up with the light and the objective lens. 3. To adjust the illumination, slowly turn the dimmer on the right side of the base until the desired intensity of light is achieved. Focusing 1. Turn the nosepiece to choose an objective. It is easiest to use the lowest magnification first (4x objective) to lo- cate and focus on the specimen. As you move up in magnification you may need to refocus the image a little each time. 2. Being focusing by first looking with one eye through the eyepiece without the diopter. Close your other eye. Use the coarse focusing knob to adjust the height of the stage until the sample comes into clear focus. Note: You may loosen the limit-stop knob (located on the read of the stage) in order to give yourself the full range of motion for fine tuning the focus. 3. Once the image is clear in your field of view, you will want to use the fine focusing knob to tune it for best results. Note: Please be careful when moving the mechanical stage if you need to recenter the sample, or if moving the stage very close to the objectives. The limit stop is designed to prevent impact between objective and slide, so when it is off you will be able to damage the microscope. Adjusting The Diaphragm 1. By changing the aperture (hole size) of the iris diaphragm, you can adjust the background brightness. Adjust the aperture of the iris diaphragm by rotating the disc underneath the stage to the desired aperture. Attaching a Camera / Changing Eyepieces 1. To remove the 10x eyepiece included on the microscope, you will need to remove the small metal screw on the ocular tube just under where the eyepiece connects to the tube. To do so, you will need a 1mm flathead precision screwdriver (much like what you would use to adjust eye glasses).

1. To adjust the stop limit on the stage, unlock it by unscrewing the lower nut. The stop limit is located at the rear of the stage. 2. Once tension is released on the nut by rotating it counter clockwise, you may adjust the upper thumb screw to move the limit up or down as desired. 3. Lock the limit stop nut back into place after the desired stage height is achieved.

pressed air or gently wiped off with a soft lens paper tissue/nonabraisive lint free cloth. - Carefully wipe off oil or fingerprints on the lens surfaces using tissue moistened with a small amount of lens cleaner (we recommend Sparkle brand optical cleaner). - Do not use Sparkle to clean other elements of the microscope. Use a neutral detergent on any plastic or painted surfaces. - Do not assemble or disassemble the microscope’s electrical components yourself without advisement from one of our technicians. Doing so will void your warranty unless by advisement of one of our technicians to do so. - After use, cover the microscope with the provided dust cover. - Keep your AmScope microscope in a dry, clean location in order to prevent rust or other damage. - To change batteries with this unit, use the included allen wrench (hexagonal shaped L-tool) to undo the door hex screw on the base. Use 3 AAA batteries with the unit. - The unit can be used as a recharger for AAA batteries specified as rechargeable only. Please do not use standard batteries in the unit with the charger plugged in to avoid damage.

Parts Specifications M149 M149A M149B M149C WF Eyepiece WF10X/18mm WF10X/18mm w/ Pointer x x x x WF10X/18mm w/ Reticle WF16X/18mm x WF20X/18mm x WF25X18mm x Plan Eyepiece P5X P10X P16X DIN Achromatic Objectives 4X/0.10 x x x x 10X/0.25 x x x x 40X(spring)/0.65 x x x x 60X(spring)/0.85 100X(spring, oil)/1.25 Plan Objectives 4X 10X 40X(spring) 100X(spring, oil) 45 Degree Viewing Head Binocular Sliding, 360 Degree Swiveling Trinocular Sliding, 360 Degree Swiveling Monocular, 360 Degree Swiveling x x x x Diaphragm Iris Diaphragm Disc x x x x Illumination Halogen Light w/ Dimmer LED Light w/ Dimmer x x x x Lamp 6V/20W 6V/30W LED x x x x Filter Blue/Yellow/Green

powers 20x EP20X23 Obtaining 80x, 200x, 800x, and 2000x magnifica- tion powers 25x EP25X23 For obtaining 250x and 2500x magnification pow- ers 10x w/ Pointer EP10X23P For easier identifying of objects 10x w/ Reticle EP10X23R For measuring objects Objective 2X A2X For obtaining 20x and 32x magnification powers 5X A5X For obtaining 50X and 80X magnication powers 20X A20x For obtaining 200x and 320x magnification powers 60X A60X For obtaining 600x and 960x magnification powers Plan 4X PA4X For obtaining higher clarify in images Plan 10X PA10X For obtaining higher clarify in images Plan 40X PA40X For obtaining higher clarify in images Plan 100X PA100X For obtaining higher clarify in images Camera CMOS Digital MU035 (350k) MU130 (1.3mp) MU300 (3mp) MU500 (5mp) MU800 (8mp) MU900 (9mp) MU1000 (10mp) To capture images, video, or view live display on a computer (PC/Mac OS X) Calibration Micrometer MR400 To calibrate the camera software for on screen measurements CCD TV/Video (Trinocular Only) CCD-NP To view live display on a television (RCA) Case Aluminum Case AC-B100 For carrying microscope around safely

Magnification Marks (Color Ring) DIN Achromatic Objective (195mm) 4X A2X Air 45 Red 10X A5X Air 45 Yellow 40X A20x Air 45 Light Blue 60X A60X Air 45 Deep Blue 100X A100X Cedar Oil 45 White Plan Objective (195mm) Plan 4X PA4X Air 45 Red Plan 10X PA10X Air 45 Yellow Plan 40X PA40X Air 45 Light Blue Plan 100X PA100X Cedar Oil 45 White Eyepieces Type Widefield Eyepiece Medium Plan Eyepiece Magnification 10X 15X 20X 5X 10X 16X Field of View Φ18 Φ13 Φ11 Φ18 Φ18 Φ15

1. 220V~240V power supply: 220V~240V ±10%, 50Hz This electrical system is CE and GS certified 2. 100V~120V power supply: 100V~120V ±10%, 60Hz This electrical system is UL certified. All units come standard as 110V units unless an upgrade to a 220V system is requested. Upgrade fee is dependent on which unit is purchased. This unit is also capable of using batteries and charging rechargable batteries. It uses 3 AAA batteries. When plugged in with batteries in the unit, the batteries will be charged. Note: Please do not use the recharging feature with standard batteries, as damage to your unit can occur.

Total Magnification Total magnification of a microscope is calculated by the magnification of the objective multiplied by the magnification of the eyepieces. -Ex: (10x Eyepieces) x (4x Objective) = 40x Total Magnification Field of View Linear field of view of the eyepiece divided by the magnification of the objective Numerical Aperture (N.A) Calculated by n Sin α (max), the Numerical Aperture (N.A) is an important parameter that marks the fe- taures of the objective and condenser’s image quality and resolution. The “n” is a refractive index of the medium (air or immersion cedar oil) between the omjective lens and the specimen. The “α” is 1/2 of the angle between the aperture on the objective and path of light. The larger the N.A, the higher the resolution of the objective (and better quality of the image). Object to Primary Image Distance The distance between the object plane and the primary image plane. The conjugate distance is fixed. Mechanical Tube Length The distane between the objective shoulder and the ocular shoulder

of view is darker The nosepiece if misaligned Turn the nosepiece until it clicks into place Stains or dust has accumulated on the objective, eyepieces, or base lens Clean all lenses with lens cleaner or a lint free non- abraisive cloth Obstructions are observed in the field of view Stains, dust, or dirt has accumulated on the speci- men Clean the slide or use a new specimen if sample is destroyed Stains, dust, or dirt have accumulated on the lens Clean the lens Unclear Image There is no cover slip on the slide Add a cover slip. The objectives are designed for use with a 0.17mm cover slip, so it is a requirement to use one for proper images. The cover slip is not standard sized Replace the cover slip with the appropriate 0.17mm thickness slip The aperture is not open to an appropriate diam- eter Adjust the aperture to have the light just larger than the size of the condenser Stain or dust has accumulated on the lens in the inlet of the head Clean the lens with lens cleaner or a nonabraisive lint free cloth, as well as spray with compressed air One side of the field of view is dark or the image moves while focusing The specimen slide is not fixed Secure the slide to the stage with clips The nosepiece is not in the right position Turn the nosepiece until it clicks into place The field of view is not bright enough The iris disc diaphragm is not big enough Rotate the iris disc diaphragm to allow more light to travel Stains, dust, or dirt has accumulated on the con- denser, objective, eyepieces, or base lens Thoroughly clean tall lenses with lens cleaner or a lint free nonabraisive cloth

Symptom Cause Remedy OPTICAL ISSUES The color of the image is not accurate The brightness adjustment knob is not in the right position Adjust the brightness knob to a higher or lower setting for color clarity No filter is used or filter is in use Remove color filter if natural light is desired, or insert desired filter MECHANICAL ISSUES The objective touches the cover slip The cover slip is not standard sized Replace the cover slip with the appropriate 0.17mm thickness slip The limit-stop is set too high or not engaged Be careful to avoid contact between objective and the slide when the limit stop is not engaged. To reengage, focus the sample, then lock the limit stop into place to set max height at a safe but us- able distance. Unable to move the slide smoothly The slide is not secured correctly Adjust the slide to use the stage clips and secure the sample The mechanical stage is not properly secured Tighten the mechanical stage screws to better secure the stage Focus knob does not turn The tension knob is too tight Loosen it by adjusting the tension ring inside the coarse focus knob counterclockwise (close to the arm of the microscope on the left of the microscope) Stage declines by itself The tension knob is too lose Tighten it by adjusting the tension ring inside the coarse focus knob clockwise (close to the arm of the microscope on the left of the microscope) The focusing knob won’t raise the stage Limit-stop is engaged Disengage the limit stop on the rear of the stage of the micro- scope ELECTRICAL ISSUES The bulb/light source flickers The bulb is close to burning out Please contact us in regards to this issue. LED lights do not burn out, so there may be another electrical issue The microscope does not light up The microscope is unplugged Insert the plug into the wall socket to achieve electrical illumina- tion