Leica TCS SP8 Quick Start Manual

microscope stand. 2. Turn on the Scanner Power (1) on the front of the Compact Supply Unit (CSU) 3. Turn on the Laser Power (2) 4. Enable the lasers by turning the key from the Off position to the On position (3). 5. Turn on Workstation and Monitor 6. Log into u ...

2. Lower the objective to the lowest position, remove specimen and clean all of the objectives 3. Save all unsaved data and exit LAS X. NOTE: Do not turn off scanner and/or CTR control box for the microscope before the software is closed 4. Turn off the switches on the front of the laser supply un ...

Scan Parameters Light Path Image ...

1. Click on the “+” in the Laser Lines Box in the Beam Path Settings Window to directly open the main Laser Control Window 2. Activate the lasers you require 3. Alternatively, click on the Configuration tab and the Laser icon to open the main Laser Control Window NOTE: Only turn on the laser(s) with the appropriate laser line(s) ...

1. Customize the USB Control Panel - assign various parameter and sensitivities to the various knobs of the control panel 2. Specifications of the Objectives equipped on the microscope Save and Load the customized settings Alternatively , click on the Control Panel Icon in the Beam Path Settings Window as a sho ...

3. Dye Database with the excitation and emission spectra of common fluorochromes Emission spectrum from a lambda scan can be added to the dye database. Many manufacturer’s will also provide the data for fluorophores that can added to the database (see LAS AF Help) Another great resource is the Leic ...

Option #1: Manual setting of the beam path configuration 1. Click On to activate the lasers 2. Adjust the laser intensity of the appropriate laser line(s) by moving the slider up or by directly entering the level (start low as a suggestion). 4. Use Autoselect to select the beam splitter (inactivate and manually select if BSC is red) 3. ...