ATP Electronics LBY-420 Instructions Manual

2. Preparation for Use 3.Basic Operation 4.Clock Setting 5. Result Thresholds 6. Sample Measurements and Test Results 7. Transferring the Test Results to PC 8.Instrument Beeps 9. Faults 10.Technical Parameters

1. Principle of Operation ATP bioluminescence detector converts invisible ATP concentration to visible light output by bioluminescent chemistry technology, to display a quantitative result of microbial population indirectly. The result is a number from 0 to 9999 in Relative Light Units(RLUs). RLUs are not a kind of tangible unit of light measurement, but they can provide a real method to the ATP bioluminescent test. In this application, 1 RLU is roughly equivalent to 1fmol of ATP. RLU value is compared with the result threshold set by user to offer an overall result, which is pass , caution or failed In order to ensure that you can get timely and accurate testing result by ATP bioluminescence detecting system, please note the following: Before you begin, please read this user's manual carefully. ATP bioluminescence detector should be auxiliarily used with the ATP sampling swab.

Before you begin, please ensure you have read this user's manual carefully and understood the system composition and method of application of this instrument. Please confirm the integrity of the product you bought according to the elements notedin the user's manual. mainfram front and back AA batteries or rechargeable batteries Data line

Liquid Crystal Display：display test procedure and result Unit lid：kill the light Lable：display the instrument type and serial number Battery compartment：fit two AA batteries Up button：check last test result, and menu selection Down button：check next test result, and menu selection Start button：start test Threshold set button：set threshold Time set button：set time Power on/off button：Power on/off the instrument Special function button：Special function, such as enter delete interface

Swab pocket: insert ATP sampling swab Data interface: transfer the data to PC by data cable.

number “1” after testing code refers to group one, and “2” refers to group two. For example, “048 2” can be regarded as the 1048th sample. Fitting the batteries The instrument is designed to operat from 2 AA batteries. The batteries are fitted by openning the battery compartment cover on the back of the instrument, and inserting two batteries with the positive end towards top of the instrument. After fitting the batteries, the instrument powers on automaticly, and enters into clock setting mode. Date and time setting is discribed in Section 4. Caution: be careful not to swap the batteries’ poles over,

Tip: for better results, please use the batteries with good quality and replace them as soon as they become low. 3. Basic operation 3.1 Power on the instrument Press the button, and the instrument will beep once and display the startup picture. Afterwards, the instrument will perform an internal self-calibration check programme for 30 seconds. Tip: If the clock is not set, the instrument will enter the time and date set-up mode firstly (see section 4) and then perform its self-calibration when the clock is set. 3.2 Internal self-calibration When the instrument is turned on (see section 3.1), it performs an internal self-calibration check, with the display counting down from 30 to 0 seconds:

the instrument and the lid must remain closed. If the icon is flashing, close the lid. The instrument will automatically perform the self-calibration routine (the flashing CAL icon) under the following circumstances; a) When the instrument is in continuous operation for a prolonged period of time (typically >30 minutes), and b) The instrument is used in an environment where the temperature changes significantly (typically >5℃). If the E1 error code was showed in self-calibration, it means the the temperature is higher than operating temperature range, and it may caused by the reasons as followed:

and b) The instrument is stored at the temperature exceeding normal range:to make the instrument adopt to normal temperature range, and c) the instrument is damaged or in fault. 3.3 Ready for use： Once the instrument has successfully performed its self-calibration, it is ready to perform a measurement: The detection procedure is discribed in Section 6.1 3.4 Power off the instrument Press the button. The instrument will beep once and the display will go blank. TIP: the button is disabled while the instrument is

3.5 Power Saving Standby Mode If the unit is turned on and has stood by for more than 10 min(on operation for more than 10 min), it will automatically enter a power saving standby mode. To turn the unit back on, please press the button 3.6 Low Battery Indicator The icon indicates the state of the batteries ------------------------------------------------------------------------------- Not visible High Visible Low, replace soon Flashing Flat, replace now When the batteries are flat, the unit will flash the icon,beep three times, and then automatically turn off. TIP:if the batteries are too flat, the unit will not power on. Store the instrument in a cool dry place when not in use, as elevated temperatures will shorten the battery life.

With the unit turned on and ready for a measurement, press button to display the current date and time. To change the time and date, press the button. Then use the and buttons to change the flashing value, and the button to accept each new value in turn. TIP: press button at any point will cancel this set-up mode, leaving the time and date unchanged. When batteries are fitted firstly or replaced, the instrument powers on automaticly, and enters into clock set-up mode. Once the clock is set, the unit will continue with its internal self-calibration. If the time is set incorrectly, the instrument will beep once and display wrong time after the button is pressed, and return to clock set-up mode.

The instrument can store up to 100 thresholds (0 to 99),each of which defines a pair of upper and lower thresholds for the measurement result. Note: the different thresholds indicate specific sanitary requirement of different testing sample. For the details how to choose the thresholds, please contact your local hygiene and epidemic prevention department, or set your own thresholds. 5.1 Changing the Thresholds Number With the instrument turned on and ready for a measurement, the thresholds number can be changed by pressing the button, and use the and buttons to change the flashing thresholds number, then press the button to accept the new value.

leaving the thresholds number unchanged. 5.2 Changing the Thresholds Range Press the button, use the and buttons to select the required thresholds number , and then press the button. Afterwards, use the and buttons to first change the value of the upper threshold followed by the button; and then to change the value of the lower threshold followed by the button to store the new values: TIP: Pressing the button at any point will exit this set-up mode, leaving both the thresholds number and thresholds value unchanged. 6. Sample Measurements and Test Results 6.1 Sample Measurement With the instrument turned on and internal calibration done, thepreparation of the new measurement is compeled.

the value of upper and lower threshold and test data number(68 indicates there are 68 test results saving in the instrument) TIP: When the stored test results take up more than 95% of the memory, the MEM icon will flash. When the memory is completely full, no more tests can be performed until the memory has been deleted or uploaded to the PC(see to section 6.3 and 7) To perform a sample measurement, follow the steps below: 1.Activate the ATP swab 2.Ensure that the outside of the swab is clean and dry 3.Open the lid, insert the swab into the instrument, and close the lid.

be displayed While the measurement is being performed, the display shows the new test number while the timer counts down from 15 to 0 seconds: TIP: For consistent results, always keep the unit upright and steady while it is performing a measurement to ensure that the liquid in the ATP swab device is at the bottom of the tube. When the measurement is complete, the new test reading and pass/caution/fail result (see section 5) are displayed. When the icon flashes, take out the swab, and press the button, and the instrument will get ready to the next measurement.

―――――――――――――――――――――――――― Test result≤ lower threshold Test result＞lower threshold≤ upper threshold Test result＞upper threshold . ―――――――――――――――――――――――――― TIP: After measurement, make sure to remove the swab to prevent the liquid leakage of swab and damage the instrument 6.2 Viewing Stored Test Results With the unit ready to perform a reading, the previous stored test results can be viewed by pressing the and buttons:

icon lit and the test number flashing. Press the button to scroll backwards through the stored test results and the button to scroll forwards. Press the button to view the time and date that the test was performed. To exit the results review mode, please press the button.

The results memory can be completely deleted by entering the results review mode (see section 6.2 above) and then pressing and holding down the button for more than 2 seconds. The display then shows the total number of results that will be deleted, with the DELETE? Icon flashing. The delete function is completed by pressing and holding down the button for more than 2 seconds, or can cancelled by pressing any other button. 7. Transferring the Test Results to PC CD-ROM data processing software which is randomly equipped can be used to upload the stored results to PC. 7.1 Install transmission procedure software Insert CD into PC, then install according to installation tips.

please open CD-ROM folder from “my computer”, then manually apply for installation. After installing, please press transmission shortcut key for transferring. 7.2 Data transmission function Run transfer function by “start” button, a dialogue box will be opened as follows:

USB cable (provided by the unit); （1）Connect one joint of cable to USB Mini interface; （2）Connect the other joint of cable to USB port on the computer; Next, choose appropriate connection port from alternative menus:

blank text document: Tips: Excel document may be safer, because its data can not be modified, so you must install Microsoft Excel on the same computer, thereby saving the results as Excel form. The next step is naming the report. The document name must be exclusive, and the used document name can not be reused. In the end, press “ ” button to power on the instrument, and then select the start button in PC programme to start the tranfer after self-inspection.

successful, “PC”PC will be displayed on the LCD of instrument. The results can be tranferred to and saved on PC at this moment, and 2000 results can be saved in 1 min. Warning: Excel is forbidden during the transfer and saving of data, for the possibility of destroying data. After transfer done, take off the data line, and push back the black rubber lid to the in situ, and the unit lid can be closed tightly. 7.4 Document type of data results The transmissive detection results are saved on Excel document or text document (see chapter 7.2). Document forms are same:

Unit No. —Unit serial number(see the labels behind the instrument) Testing serial number —Testing No. Date — Date of operation detection Limit No. —Limit serial number for detection RLU —RLUs reading detection result Lower limit —Low（↓）set limit value Upper limit —Upper（↑）set limit value Note —Spare area If necessary, users can add comments of each result on the spare area.

During normal use, the unit emits a variety of different beeping sounds: Beep type Possible causes ----------------------------------------------------------------------------------------------------------------- Short high- pitched tone Unit turned on or off Sample measurement started Connect with PC Results memory being deleted Long high-pitched tone Unit self-calibration complete Sample measurement complete Results memory delete complete Long low-pitched tone Invalid date entered Invalid program threshold limits entered 9. Faults In case of a fault, it is generally caused by battery; if the detector can not be turned on or turned off, or unit turns off unexpectedly, they are mainly caused by power off or loose battery; if there is a detector whose battery is in good condition, but it can not work normally, please contact with manufacturer for solution. The followings are the typical cases and their possible causes. √ represent users can solve the problems by themselves × represent the problems may be solved by technicians, please contact with manufacturer for solution.

√Unit not used for more than 10 minutes and automatically turns off into standby mode Keypad button has no effect when pressed √ Some buttons only work when selecting particular unit functions × Keypad or unit damaged or faulty Measurement reading always shows zero RLU, or is much lower or high than expected √Incorrect use of √CariScreen Swab device √CariScreen Swab devices are out-of- date √Unit being used in an unstable environment turn off unit and then back on again √Protective pocket dirty or severely scratched × Unit damaged or faulty USB interface does not appear working condition √inserted unit is incorrect ×PC serial interface or system software error √PC software installation error or select error ×wire or interface damaged × Unit damaged or faulty

Detection precision: 2X10-16mol ATP Lower limit of detection: ≤1.0 CFU/ml Detection range: 0~9999 RLU （relative light unit） Coliforms: ≥1-106CFU Detection time: 15s Detection interference: ±5﹪or ±5 RLUs Background value: ≤2RLU Detection mode: RLU or coliforms screening Number of settable limit: more than 100 Storage size: more than 2000 detection results USB interface: the results can be transferred to PC computer Instrument dimensions（W×H×D）: 192mm×87mm×34mm Instrument weight（Battery included）: 265 g Range of operating temperature: 5℃－40℃ Relative humidity range: 20-85﹪ Battery: AA battery Resting state（20℃）: 6 months

the surface is inspected and the results recorded. The same wiping technique should be used for testing, and five different times should be selected for testing at the same location. This shows the standards that can be achieved after regular cleaning. 1. The limit values of the key points are as follows: key point the first time the second time The third time The forth time The fifth time 1 Pollution 524 857 489 763 615 2 clean 10 15 8 19 10 3 Strict clean 4 3 0 2 1 After the reference data of the key points is collected, the following operations can be used to obtain the RLU standards for the passing, warning, and failure of the routine inspection work: 1. By calculating the RLU average for cleaning or rigorous cleaning, this value is your standard limit.

12 2. It is obtained by multiplying the pass value by 2 by the limit value. For example: not passed = 12.4 × 2 = 24.8 ≈25 3. Alert The limit value is between passing and not passing the limit value. Some users choose not to set a warning value, while others set the non-limit value to be higher than the limit value. Example of setting a warning value: Pass standard = 12 Caution Standard = 13 to 25 Fail standard (===26) Third, the recommended limit value setting When the instrument is first used, we have a set of recommended universal limits. These limit values are determined according to GB/T4789.2-2003 "Food Hygiene Microbiological Test - Total Number of Colonies", which is determined by SAS statistical software analysis to determine the total number of colonies and the regression equation of the ATP instrument for detecting RLU values. 2.Reference limit ATP Reference limit Pass the warning not pass RLU <15 15－29 >30

stainless steel 8 30 Cutter-cheese stainless steel 8 30 Preparation table stainless steel 8 30 Auxiliary tools such as pliers stainless steel 8 30

material pass Fail Chicken fork stainless steel 15 30 Preparation table stainless steel 15 30 Service area - 15 30 oven stainless steel 15 30

stainless steel 15 30 Sandwich preparation area material pass fail Knife stainless steel 15 30 Working surface stainless steel 15 30 Preparation board plastic 15 30

material pass Fail Knife stainless steel 15 30 Mincer stainless steel 15 30 Working surface stainless steel 15 30 Weighing apparatus stainless steel 15 30 Fish material Pass Fail Chopping board plastic 30 100 Preparation table stainless steel 15 30 Knife stainless steel 15 30

Pizza material Pass Fail Spice pot stainless steel 15 30 Working surface stainless steel 15 30 Preparation table - 15 30