Shodex IC SI-52 4E Operation Manual

1 Operation Manual Shodex IC SI-52 4E （Please read this manual carefully to achieve the best and consistent column performance for a long time） Important Handling Instructions Caution! · Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column and understand their proper handling methods to prevent potential health hazards or death from occurring. · Please wear appropriate personal protective equipment such as lab goggles and gloves when handling organic solvents and acid and alkaline reagents. Avoid any direct physical contact to prevent chemical injuries. Before Using the Column (1) Please visually inspect the package and outside of the column for any damage. (2) Please check if product name and serial number written on the column package, column name tag, and enclosed Certificate of Analysis (CoA) are matching and correct. 1. Introduction Thank you for purchasing the Shodex product. Shodex IC SI-52 4E is a suppressor type ion chromatography column for analysis of anions, using sodium carbonate eluents. The column is suitable for the simultaneous analysis of oxyhalides including chlorite, chlorite, and bromate, and standard inorganic anions including fluoride, chloride, nitrite, bromide, nitrate, phosphate, and sulfate. 2. Column Components 3. Column Specifications Product Code Product Name Column Size (mm) Particle Size (μm) Theoretical Plate Number (Per Column) I.D. Length F6995260 IC SI-52 4E 4.0 250 5 ≥ 14,000 F6709626 IC SI-92G 4.6 10 9 (Guard Column) Base Material : Spherical porous particles of polyvinyl alcohol modified with quaternary ammonium group Column Housing : PEEK Screw Type : Internally-threaded type No.10-32 UNF Shipping Solvent : 3.6 mM Sodium Carbonate Solution

2 4. Usable Conditions Usable solvents are listed below. (1) In general, sodium carbonate solution is used. (2) The standard eluent is 3.6 mM sodium carbonate solution. The higher the concentration of sodium carbonate solution, the faster the elution of ions will be. (3) Up to 10 % (v/v) acetonitrile or methanol is usable. Attention! · Use the column within above stated flow rate, pressure, and temperature ranges. Using the column outside the given range may damage the column and lower its performance. · Column pressure is influenced by the eluent composition, flow rate, and column temperature. When changing the eluent compositions, adjust the flow rate and column temperature so that the column pressure remains below the usable maximum pressure. 5. Eluent Preparation (1) Degas the eluent fully to prevent the formation of air bubbles. (2) Presence of small debris or insoluble substances may result in deterioration of the column and/or they appear as noise on the chromatograms. Filter the eluent with a 0.45-μm disposable filter to prevent the problems. Attention! · Whenever water is required, use ultra-pure water freshly generated by a water purification system or water from a newly opened HPLC grade distilled water bottle. Use HPLC grade organic solvent whenever possible. Solvents left in an opened bottle for a long time should not be used. The content may have been changed, absorbed moisture, or has been contaminated. · Always use freshly prepared solvents. Solvents stored for a long time may have changed their compositions and may influence elution patterns and/or damage the column. Note · Use of on-line degasser is recommended. 6. Sample Preparation (1) Filter the sample solution using disposable 0.45-μm filter to prevent the column from clogging or deteriorating. (2) Recommended sample injection volume is less than 20 μL per column. (3) For the samples containing proteins, remove the proteins prior to the sample injection. The use of an ultrafiltration membrane is recommended. (4) For the samples containing hydrophobic impurities, remove the impurities prior to the sample injection. The use of a solid phase extraction is recommended. Note · Use of guard column is recommended to protect the analytical column. 7. Column Usage Procedure 7.1 HPLC System Preparation Wash entire LC system prior to the column installation, including all flow-lines and sample loop by switching the valve, and then replace the washing solution with the eluent to be used. If desired new eluent has low miscibility/solubility to the eluent of previous analysis, first use the eluent that is miscible/soluble to both eluents, and then replace it with the desired eluent. Attention! · If the eluent left in the system is not compatible with the column to be used, it may damage the column. · A drastic change in the eluent compositions may remove substances adsorbed on the system and they may enter and deteriorate the column. Product Name Flow Rate (mL/min) Maximum Pressure (MPa) (Per Column) pH Range Temperature (°C) Recommended Maximum Recommended Range IC SI-52 4E 0.8 1.0 15 3 - 12 45 20 - 60 IC SI-92G - - -

3 7.2 Column Installation (1) Connect the column to LC system by following the “flow direction arrow” (➡) indicated on the column name tag. If guard column is used, position the guard column in front (before the inlet) of the analytical column. (2) Make sure to insert the tubing all the way to the end fitting and secure it with the male nut. It is important that there is no extra space between the tubing and the column side of the end fitting. Presence of an extra space will let the sample to spread out and may result in wide peaks. (3) Set the initial flow rate at less than 0.3 mL/min and start the system. If using the column at an elevated temperature, keep a low flow rate until the temperature of the column reaches to the set temperature, and then gradually increase the flow rate to the desired. Caution! · Verify that there is no solvent leak. It may cause electronic leakage, rust, and/or chemical injury. Attention! · Make sure not to let air bubbles enter the column while installing the column. The air bubbles may damage the column. · When restarting the system after column installation or after holding the eluent flow, start the system at less than 0.3 mL/min. A rapid increase in pressure can damage the column. · If the column was used at an elevated temperature, lower the flow rate to less than 0.3 mL/min at the end of analysis. Then, turn off the column oven, and let the column temperature return to room temperature before stopping the pump. If the pump was stopped while the eluent inside the column was still hot, as the eluent temperature decreases, its volume also decreases. This may result in creating an empty space in the column and deteriorates the column. Note · It is recommended to set the pump limiter to avoid exceeding the maximum pressure. 7.3 Solvent Exchange When replacing the solvent, start the system at less than 0.5 mL/min. Recommended solvent volume to introduce at each step is 3 to 5 times of the column volume. 7.4 Column Cleaning Problems in peak shapes and elution time changes or elevated column pressure etc. are often caused by insoluble or adsorbing components present in the eluent and reagents being deposited inside the column. These problems may be resolved by cleaning the column. If a guard column is used with the analytical column, first remove the guard column and check the performance of the analytical column alone. If the problem is solved, most likely the cause is from the guard column. In this case, clean the guard column. If the problem is not solved by removing the guard column, clean both guard and analytical columns. Make sure to clean the guard and the analytical columns separately. When washing the column, let the washing solution flow from the column outlet go directly into the waste container and not let the solution go through the detector. (1) Insoluble components that block the column inlet may be removed by reversing the flow direction, i.e., introducing the eluent from the column outlet, with flow rate at less than half of the recommended flow rate. (2) Follow below cleaning steps for adsorbing components. For an efficient cleaning, reverse the flow direction and use the flow rate at 0.3 mL/min.

4 Method 1: Adsorption of hydrophilic compounds Washing Solution Cleaning Time 1 H2O 25 min 2 10 x Concentrated eluent 100 min 3 H2O 25 min 4 Eluent (Return the column flow to normal direction) 100 min Method 2: Adsorption of hydrophobic compounds Washing Solution Cleaning Time 1 H2O 25 min 2 H2O/CH3CN = 95/5 20 min 3 CH3CN 100 min 4 H2O 50 min 5 Eluent (Return the column flow to normal direction) 100 min Attention! · Leaving the washing solution in the column for an extended time can damage the column. Do not suspend the column cleaning, but complete all procedures until the last step to replace it with the eluent. 8. Column Storage Remove the column from the system after replacing the in-column solvent with the shipping solvent. Securely tighten the end caps and store the column at a location with stable temperature (a cool and dark space is recommended). Refer to section 7.3 Solvent Exchange for how to replace the eluent. Attention! · Never allow inside of the column to dry. It can damage the column. 9. Column Inspection Inspection method is described in the Certificate of Analysis (CoA). Theoretical Plate Number (N) and Asymmetry Factor (Fas) were calculated using the below equations. (1) Theoretical Plate Number (N) (2) Asymmetry Factor (Fas)

5 10. Additional Warnings (1) Do not remove end fittings. (2) Do not make a strong impact on the column. Do not drop or hit the column on a hard surface. Refer to the Shodex™ website (https://www.shodex.com/) for product details and their applications. For additional assistance, contact the distributor from whom you purchased the column or contact your regional Shodex support office (https://www.shodex.com/en/support_office/list/).

6 11. Warranty (Ver. 4COLUMN) (1) Showa Denko K. K. warrants that the Shodex product, at the time of delivery to the user, will conform to the specification of the attached CERTIFICATE OF ANALYSIS, if the Shodex product is used in accordance with the attached operating manual. The foregoing warranty is exclusive and is in lieu of all other warranties with respect to the Shodex product, whether written, oral, implied, statutory or otherwise. No warranties by Showa Denko K. K. are implied or otherwise created, including, but not limited to, the warranty of merchantability and fitness for particular purposes. (2) Any claim of inconformity to the specification must be notified to Showa Denko K.K. within ten (10) days after delivery to the user. User’s exclusive remedy and Showa Denko K.K.‘s exclusive liability for such claim are limited to the replacement of the Shodex product in question. In no event is Showa Denko K.K. liable for any indirect, incidental or consequential damage arising out of in connection with the Shodex product, whether or not such damage is allegedly based on breach of warranty, negligence or otherwise. (3) No warranty is made in any of the following cases: 3-1 If the Shodex product is not used in accordance with the operating manual 3-2 If the Shodex product is remodeled by anyone other than person or firm designated by Showa Denko K.K. 3-3 If the Shodex product is disposed of 3-4 If the Shodex product is resold by the user without giving prior written notice to Showa Denko K.K. 3-5 If the performance of the Shodex product is not conform to the specification of the attached CERTIFICATE OF ANALYSIS due to any of the reasons below: a. Computer virus b. Impurities contained in the sample, reagent, gas air or cooling water provided by the user c. Breakdown or malfunction of equipment, apparatus or component used in combination with the Shodex product d. Force majeure such as fire, earthquake, flood, other natural disaster, crime, riot, act of terrorism, war or radioactive contamination (4) In no event is Showa Denko K.K. liable for 4-1 the results of analysis or preparations using the Shodex product or any portion of the same, including, but not limited to, the reliability, accuracy, efficacy and safety of said results, and 4-2 the occupational hazard in the use of the Shodex product, whether or not such use is made in accordance with the attached Conditions for use. (5) The Shodex product is for laboratory use only. It must not be used for clinical diagnosis. Showa Denko K.K. is not liable for any use of the Shodex product except laboratory use. Following names are trademarks or registered trademarks of SHOWA DENKO K.K. Shodex, AFpak, Asahipak, AXpak, CLNpak, CXpak, HILICpak, MSpak, ODP, OHpak, ORpak, RSpak, SUGAR, USPpak. Products described in the operation manual are not intended for medical use or medical applications.