Olympus CX22LED Instructions Manual

This instruction manual is for the Olympus Biological Microscopes Model CX22LED. To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of this microscope, we recommend that you study this manual thoroughly before operating the microscope. Retain this instruction manual in an easily accessible place near the work desk for future reference.

pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instruction manual, may cause harmful interference to radio communications. Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense. FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment. In accordance with European Directive 2002/96/EC on Waste Electrical and Electronic Equipment, this symbol indicates that the product must not be disposed of as unsorted municipal waste, but should be collected separately. Refer to your local Olympus distributor in the EU for return and/or collection systems available in your country.

10-1 System Diagram of Optional Accessories............................................................................ 18 10-2 Installation and Operation of Optional Accessories .............................................. 18 1 Intended Use........................................................................................................................................................................................................................... 2 2 Getting Ready........................................................................................................................................................................................................................ 2 3 Maintenance and Storage ......................................................................................................................................................................................................................... 3 1 Turning the LED Illumination ON .......... 7 3 Adjusting the Focus ................................................ 8 5 Adjusting the Diopter ........................................ 10 2 Placing Specimen on the Stage .................... 7 4 Adjusting the Interpupillary Distance..................... 9 6 Adjusting the Condenser Position and Aperture Iris Diaphragm ............................................................. 10 7 Switching the Objectives ............................ 10 8 Using the 100X Immersion Objective.................... 11 1 STANDARD COMPONENT UNITS 2 NOMENCLATURE 3 SUMMARY OF BRIGHTFIELD OBSERVATION PROCEDURE 4 DETAILED OBSERVATION PROCEDURE 5 ONE-POINT ADVICE 6 TROUBLESHOOTING GUIDE 7 SPECIFICATIONS 8 OPTICAL CHARACTERISTICS 9 ASSEMBLY 12 13,14 15 16 17 18-20 10 OPTIONAL ACCESSORIES PROPER SELECTION OF THE POWER CORD .................................................................................. 23,24 1 Filter Holder CH2-FH and Darkfield Ring CH2-DS........................................................................................................ 18 2 Reflection Mirror CH20-MM ........................................................................................................................................................................... 19 3 Eyepieces WHC15X/WHC15X-H ............................................................................................................................................................................................... 19 4 Using the eyepiece micrometer ............................................................................................................................................................................................... 20 5 Cord Hanger CX22LED-CH ................................................................................................................................................................................................................. 20

infection, clean the parts coming in contact with the specimen to prevent infection. · Moving this product is accompanied with the risk of dropping the specimen. Be sure to remove the specimen before moving this product. · In case the specimen is damaged by erroneous operation, promptly take the infection prevention measures. 2. Install the microscope on a sturdy, level table or bench. 3. Always use the AC adapter and power cord provided by Olympus. If no power cord is provided, please select the proper power cord by referring to the section “PROPER SELECTION OF THE POWER CORD” at the end of this instruction manual. If the proper power cord is not used, product safety performance cannot be warranted. 4. Always ensure that the grounding terminal of the microscope and that of the wall outlet are properly connected. If the equipment is not grounded, Olympus can no longer warrant the electrical safety performance of the equipment. 5. After operation or in case of abnormality, be sure to disconnect the power cord of the AC adapter from the connector on the microscope or from the wall power outlet. 6. This product complies with the emission and immunity requirements described in IEC61326 series. 7. The electromagnetic environment should be evaluated prior to operation of this product. Do not use this product in close proximity to the sources of strong electromagnetic radiation to prevent interference with the proper operation. Safety Symbols The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment in the safest possible manner. Symbol Explanation Indicates that the main switch is ON. Indicates that the main switch is OFF. Before use, carefully read the instruction manual. Improper use could result in personal injury to the user and/or damage to the equipment. LED (light emitting diode) safety warning The LED built in this product is basically eye-safe. However, direct viewing the light from the LED unit for extended time may cause damage to the eyes. If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the equipment may also be damaged. Always use the equipment as outlined in this instruction manual. The following indications are used to set off text in this instruction manual. : Indicates a potentially hazardous situation which, if not avoided, may result in minor or moderate injury or damage to the equipment or other property. It may also be used to alert against unsafe practices. } : Indicates commentary (for ease of operation and maintenance). CAUTION Caution

This product has been designed to be used to observe magnified images of specimens in various routine work and research applications. Do not use this product for any purpose other than its intended use. Disconnect the AC adapter before carrying or storing the microscope. 2 Getting Ready 1. A microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact. 2. Do not use the microscope where it is subjected to direct sunlight, high temperature and humidity, dust or vibrations. (For the operating conditions, see chapter 7, “SPECIFICATIONS” on Page 15.) 3. Always use the tension adjustment ring to adjust the rotation tension of the coarse adjustment knob. 4. Be sure to leave enough space around the AC adapter when installing the microscope. 5. When carrying the microscope, first make sure that the AC adapter has been disconnected and hold both sides around the hole of the arm as shown in Fig. 1 and carry it carefully. · To prevent damage, do not hold the microscope by the stage 1 or observation tube 2. Be sure to remove the specimen; otherwise, it may fall. · Do not carry the microscope with the AC adapter connected to prevent damage to the AC adapter or the microscope by hitting the output connector of the AC adapter. · Be sure to disconnect the AC adapter from the microscope when storing the microscope after use. Fig. 1 @ ² CAUTION Fig. 2 This product complies with the requirements of directive 98/79/EC concerning in vitro diagnostic medical devices. CE marking means the conformity to the directive. USA CAUTION: Federal law restricts this device to sale by or on the order of an appropriately licensed healthcare practitioner.

1. To clean the lenses and other glass components, simply blow away dirt using a commercially available blower and wipe the components gently using a piece of cleaning paper (or clean gauze). If a lens is stained with fingerprints or oil smudges, wipe it with gauze slightly moistened with commercially available absolute alcohol. Since the absolute alcohol is highly flammable, it must be handled carefully. Be sure to keep it away from open flames or potential sources of electrical sparks –– for example, electrical equipment that is being switched on or off, since such operation may cause ignition of a fire. Also remember to always use it only in a well-ventilated room. 2. Do not attempt to use organic solvents to clean the microscope components other than the glass components. To clean them, use a lint-free, soft cloth slightly moistened with a diluted neutral detergent. 3. Do not disassemble any part of the microscope as this could result in malfunction or reduced performance. 4. When not using the microscope, store it in a dry locker or cover it with a dust cover. Fig. 3 5. To clean the condenser, fully loosen the securing knob 1, then remove the condenser by lowering it with the condenser height adjustment knob 2, and wipe the front lens of the condenser. The condenser can be attached by reversing the above removal procedure (Fig. 3). 6. When disposing of the microscope, check the regulations and rules of your local government and be sure to observe them. @ ² CAUTION

}After opening the package, make sure that the correct component units for the selected set are included. The 100X objective and immersion oil are provided only with the CX22LEDRFS1 set. The objectives have been screwed in tightly to prevent them from being loosened during transportation. To remove an objective, turn it counterclockwise while holding it with a rubber sheet, etc., so that your fingers don’t slip. Eyepieces (Fixed at 10X) Stage Condenser Microscope frame · 4X, 10X, 40X, 100X (CX22LEDRFS1) · 4X, 10X, 40X (CX22LEDRFS2) Immersion oil · Provided with a set including the 100X objective. Optional Accessories · Filter Holder CH2-FH · Reflection Mirror CH20-MM · Eyepieces WHC15X WHC15X-H · Darkfield Ring CH2-DS AC adapter Power cord Binocular observation tube Objectives Revolving nosepiece · Cord Hanger CX22LED-CH CAUTION

retain them for future use. Then loosen the observation tube clamping knob and correct the orientation of the eyepieces as shown in the illustration below. 1 Revolving nosepiece/observation tube transport band 2 Stage and specimen holder protection sheet 3 Protective pad below the stage }Attach the AC adapter and power cord as described in chapter 9, “ASSEMBLY” on page 17. }For detailed description of each item, refer to the page indicated inside parentheses. NOTE FOR UNPACKING “ ”: Power ON “ ”: Power OFF Diopter adjustment ring (Page 9) Specimen holder (Page 7) Aperture iris diaphragm ring (Page 10) Filter holder Light intensity adjustment knob (Page 7) Revolving nosepiece (Page 10) Observation tube clamping knob Pre-focusing knob (Page 9) Specimen holder Y-axis feed knob (Page 7) Specimen holder X-axis feed knob (Page 7) Fine adjustment knob (Page 8) Coarse adjustment knob (Page 8) Main switch (Page 7) Accommodates a 45-mm diameter filter.

1Main switch (P. 7) 2Light intensity adjustment knob (P. 7) 3Specimen holder (P. 7) 4X-axis/Y-axis feed knobs (P. 7) 5Revolving nosepiece (P. 10) 6Coarse/fine focus adjustment knobs (P. 8) Adjust the interpupillary distance. Adjust the diopter. 7Binocular tube (P. 9) 8Diopter adjustment ring (P. 9) 9Aperture iris diaphragm ring (P. 10) aFilters (Diameter: 45 mm) (P. 5) Engage the required filters. 5Revolving nosepiece (P. 10) 6Coarse/fine adjustment knobs (P. 8) Adjust the brightness. 2Light intensity adjustment knob (P. 7) (Items Used) (Page) 1 2 4 6 a 9 3 5 7 8 Adjust the aperture iris diaphragm. 9Aperture iris diaphragm ring (P. 10) Engage the objective to be used in the light path and bring the specimen in focus. Adjust the aperture iris diaphragm. Set the main switch to “ I " (ON) and adjust the brightness. Place the specimen on the stage. Engage the 10X objective in the light path. Bring the specimen in focus

1 Turning the LED Illumination ON (Fig. 4) 1. Set the main switch 1 to “ I ” (ON). 2. Rotating the light intensity adjustment knob 2 in the direction of the arrow increases brightness and rotating it in the opposite direction de- creases brightness. The figures around the knob indicate the reference brightness. 2 Placing Specimen on the Stage (Figs. 5 & 6) Place the specimen gently. If the bow-shaped lever is returned with a strong force or the knob 1 of the bow- shaped lever is released in the middle, the slide glass may be broken. 1. Rotate the coarse adjustment knob 2 in the direction of the arrow to fully lower the stage. 2. Open the bow-shaped lever 3 outward, place the specimen by sliding the specimen glass plates on the stage from the front toward the rear. 3. After sliding the specimen glass plates all the way, return the bow-shaped lever 3 gently. 4. Rotating the upper knob which is the Y-axis feed knob 4 moves the specimen in the vertical direction. Rotating the lower knob which is the X- axis feed knob 5 moves it in the horizontal direction. · Do not move the specimen holder 6 directly by hand, this will damage the rotary mechanisms of the above knobs. · When the specimen holder reaches the stopper position, the rotation force of the above knobs become heavy. Stop rotating the knob at this time. ² @ ² @ | ³ 5 Cover glass This is the glass plate placed on the specimen. To allow the objective manifest the full performance, the cover glass thickness, which is the distance from its surface to the specimen surface, should ideally be 0.17 mm. Slide glass This glass plate should ideally have a length of 76 mm, width of 26 mm and thickness between 0.9 and 1.4 mm. Fig. 6 Placing Specimen Cover glass Slide glass CAUTION CAUTION 6

}Theses scales allow the position (coordinates) being observed on the specimen to be identified. Even after the specimen is moved, it can be returned easily to the original position. 1. The horizontal coordinate can be read at position 1 on the specimen holder. 2. The vertical coordinate can be read at the position of index line 2. Fig. 8 3 Adjusting the Focus (Fig. 8) Focusing Procedure 1. Rotate the coarse adjustment knob @ in the direction of the arrow so that the objective 3 is as close as possible to the specimen. 2. While observing the specimen through the eyepieces, slowly rotate the coarse adjustment knob 1 in the opposite direction to the arrow to lower the stage. 3. When coarse focusing of the specimen is obtained, rotate the fine adjustment knob 2 to adjust to precise focus. Working Distance (WD) }The WD refers to the distance between each objective and the specimen, when precise focus of the specimen is obtained. Objective Magnification 4X 10X 40X 100X WD (mm) 27.8 8.0 0.6 0.13 Fig. 9 Adjusting the Tension of the Coarse Adjustment Knob (Fig. 9) 1. The tension of the coarse focus adjustment knob has been designed adjustable with a ring. Insert the tip of a large flat-blade screwdriver into the groove 2 on the tension adjustment ring 1 and rotate the ring. Rotating it clockwise (in the direction of the arrow) increases the tension and counterclockwise decreases the tension. 2. If the stage descends on its own or if the specimen gets out of focus quickly even when it is brought into focus using the fine adjustment knob, it means that the tension of the coarse adjustment knob is too low. Turn the ring 1 in the direction of the arrow to increase the tension. ² @ ² @ ³ ² @ ³

}The pre-focusing knob controls the mechanism for preventing collision between the specimen and objective. 1. After bringing the specimen into focus, turn the pre-focusing knob 1 inside the hole of the arm so that the pre-focusing mechanism hits the stage guide. 2. To provide a certain margin for focusing, rotate the knob by about half turn backward from the stopped position. If the function of this mechanism is not required, set the pre-focusing knob 1 at the highest position. If the pre- focusing knob 1 is not set at the highest position, the specimen may not be focused. @ CAUTION 4 Adjusting the Interpupillary Distance (Fig. 11) }The interpupillary distance adjustment is to regulate the two eyepieces according to that between your eyes so that you can observe a single microscopic image through two eyepieces. This helps to reduce fatigue during observation. While looking through the eyepieces, move both eyepieces until the left and right fields of view coincide completely. When the both of eyepieces are lined in the horizontal position, the posi- tion of index ridge on the right side eyepiece sleeve 1 indicates the interpupillary distance value. }Note your interpupillary distance so that it can be quickly duplicated. }You can move the eyepieces upward and downward from the position where both eyepieces are in a straight line with maximum interpupillary distance. Fig. 11 @

(Fig. 14) }The condenser is usually used in the highest position. If the entire observed field of view is not bright enough, brightness may be improved by lowering the condenser slightly. 1. Rotate the condenser height adjustment knob 1 to move the condenser to the highest position. 2. The aperture iris diaphragm ring 2 has an objective magnification scale (4X, 10X, 40X, 100X). Rotate the ring so that the magnification of the objective in use faces frontward. 7 Switching the Objectives (Fig. 15) Fig. 15 Hold and rotate the revolving nosepiece 1 so that the objective to be used comes exactly above the specimen. ² @ @ Fig. 12 Fig. 13 5 Adjusting the Diopter (Fig. 12) }The diopter adjustment is to compensate for the difference in eyesight between your eyes. 1. While looking through the right eyepiece with your right eye, rotate the coarse and fine focus adjustment knobs to bring the specimen into focus. 2. While looking through the left eyepiece with your left eye, rotate only the diopter adjustment ring 1 to focus on the specimen. Using the Eye Shades (Fig. 13) When Wearing Eyeglasses Use the eye shades in the normal, folded-down position. This will prevent the eyeglasses from being scratched. When Not Wearing Eyeglasses Extend the folded eye shades in the direction of the arrow to prevent extraneous light from entering between the eyepieces and eyes. @

(Fig. 16) }The designated immersion oil should be applied to the top lens of the 100X immersion objective. Otherwise, the observed image will be unable to be focused on. Always use the immersion oil supplied by Olympus. 1. Focus on the specimen using all objectives, starting from the lowest- power objective to higher-power objective. 2. Before engaging the immersion objective in the light path, place a drop of the provided immersion oil onto the specimen at the area to be observed. 3. Rotate the revolving nosepiece to engage the immersion objective and rotate the fine adjustment knob to bring the specimen into focus. Since air bubbles in the oil will affect the image quality, make sure that the oil is free of bubbles. To remove bubbles, rotate the revolving nosepiece slightly to move the oil-immersed objective by one reciprocation or two. }The condenser of this microscope manifests the full performance when oil is placed between the slide glass and the front lens of condenser. If oil is not applied there, the observation image may become slightly dark. 4. After use, remove oil from the objective front lens by wiping with gauze slightly moistened with absolute alcohol. Caution in use of immersion oil If immersion oil enters your eyes or contacts your skin, immediately take the following treatment. Eyes: Rinse with fresh water (for 15 minutes or more). Skin : Rinse with water and soap. If the appearance of the eyes or skin is altered or pain persists, immediately see your doctor. CAUTION CAUTION CAUTION

To track an image moving leftwards (or to move the specimen image rightwards): Direction of moving the image Total Magnification The size of the specimen image for observation is obtained by multiplying the eyepiece magnification by the objective magnification. This value is referred to as total magnification. Example: Eyepiece (10X) x Objective (40X) = 400X Resolution Resolution determines how finely a lens is able to distinguish the details of a specimen. The resolution of a microscope is mainly determined by the ability of the objective and is scarcely related to that of the eyepieces. The only function of the eyepieces is to magnify an image already resolved by the objective. Although both configurations above provide the same total magnification, the higher magnification of the objective yields a better specimen resolution. Field Number (FN) The field number is the diameter of the image observed through an eyepiece, represented in millimeters. 10X eyepiece: 20 mm WHC15X: 12 mm Actual Field of View The actual field of view is the size on the specimen actually observed in the microscope. This yields approximate size of the actual specimen. Actual field of view = Field number/Objective magnification When the 10X eyepiece above and 10X objective are used, the actual field of view is equal to: Actual field of view = 20/10 = 2.0 mm Move the slide glass to the left. To move the specimen image upward: Move the slide glass down. }The image observed through the microscope moves in directions opposite to the actual up-down and left-right movements of the specimen. Actual field of view: 2.0 mm Approx. 1.0 mm, about half of actual field of view.

please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list, please contact Olympus for assistance. Problem Cause Remedy Page 10 1. Uneven brightness in observation field. The objective is not engaged in the light path. Engage the objective into position until it clicks. The condenser is too low. Raise it to the upper limit. The objective, eyepiece, condenser and/ or window lens are dirty. Clean them thoroughly. 10 3 2. Dust or stains are visible in obser- vation field. The eyepiece, condenser, window lens and/or specimen glasses are dirty. Clean them thoroughly. 3 3. Observation image glares. The condenser is too low. Raise it. The condenser iris diaphragm ring is stopped down excessively. Adjust the aperture according to the ob- jective magnification. 10 10 4. Observation image is whitish- blurred or unclear. The objective is not engaged in the light path. Engage the objective into position until it clicks. The objective, eyepiece, condenser and/ or specimen glasses are dirty. Clean them thoroughly. 10 3 Immersion oil is not used with an immer- sion objective. Use immersion oil. Bubbles are mixed in the immersion oil. Remove the bubbles. The specified immersion oil is not used. Use the immersion oil supplied by Olympus. 11 11 11 5. Part of image is defocused or image looks like it’s flowing. The objective is not properly engaged in the light path. Engage the objective into position until it clicks. The specimen is not set properly on the stage. Set the specimen correctly on the stage and secure using the specimen holder. 6. High-magnification objective touches specimen just before coming into focus. The specimen is upside down. Set the specimen correctly with the cover glass on the top. 7. The tension of coarse adjustment knob it too high. The coarse adjustment knob tension ad- justment ring is set too tight. Loosen the ring to adjust to proper tension. 10 7 7 8 8. Focusing is impossible (because the stage cannot be raised). The pre-focusing knob is positioned too low. Raise its position. 9 9. The stage lowers by its own weight or focusing is lost due to slippage of the knob. The coarse adjustment knob tension adjustment ring is set too loose. Tighten the ring to adjust to proper ten- sion. 10.Coarse focus adjustment cannot lower the stage low enough. The condenser is too low. Raise it. 8 10 11. Fields of view of two eyes do not match. The interpupillary distance is not adjusted properly. Adjust it properly. 9 Diopter compensation for the two eyes is not set. Adjust it correctly. The left and right eyepieces are different. Replace one of them so that the left and right eyepieces are identical. 10 --

an objective is switched to a higher- magnification objective. The specimen is upside down. Set the specimen correctly with the cover glass on the top. The cover glass is too thick. Use a cover glass with thickness of 0.17 mm. 13. The LED illumination does not light. The AC adapter or power cord is un- plugged. Plug it securely.

Infinity Correction optical system 2. Illumination Built-in 0.5 W LED illumination system. Rated voltage: 5-6 V 0.5 A (AC adapter: 100-240 V 50/60 Hz 0.4A) Power consumption: 1.7 W 3. Focusing mechanism Stage height adjustment mechanism. Fine adjustment scale: 2.5 μm per graduation Fine adjustment stroke: 0.3 mm per turn Total stroke: 20 mm. Pre-focusing knob provided, coarse adjustment knob tension adjustable. 4. Revolving nosepiece Quadruple positions fixed (Front oriented) 5. Binocular observation tube Field number 20 Tube tilting angle 30° Interpupillary distance adjustment range 48 to 75 mm 6. Stage Size 120 x 132 mm (with mechanical stage) Movement range 76 (X-axis) x 30 (Y-axis) mm Specimen holder Holds a single specimen. 7. Condenser Type Abbe condenser N. A . 1.25 (when immersed in oil) Aperture iris diaphragm Built in 8. Dimensions & weight 154(W) x 392(H) x 238(D) mm, approx. 6 kg 9. Operating environment · Indoor use. · Altitude: Max. 2000 meters · Ambient temperature: 5° to 40°C (41° to 104° F) · Maximum relative humidity: 80% for temperatures up to 31°C (88°F), decreasing linearly through 70% at 34°C (93°F), 60% at 37°C (99°F), to 50% relative humidity at 40°C (104°F). · Supply voltage fluctuations; Not to exceed ±10% of the normal voltage. · Pollution degree: 2 (in accordance with IEC60664-1) · Installation/Overvoltage category: II (in accordance with IEC60664-1) 10.Transport and Storage environ- ment · –25 to +65 °C · 0 to 90 %RH

Total Power Focal Depth (μm) Actual Field of View Remark Plan objective (FN 20) 4X 0.10 27.8 – 3.36 40X 175.0 5.0 10X 0.25 8.0 – 1.34 100X 28.0 2.0 40X 0.65 0.6 0.17 0.52 400X 3.04 0.5 100XO* 1.25 0.13 – 0.27 1000X 0.69 0.2 Oil immersed Legend Working distance (WD) : Distance between the top surface of the cover glass and the objective extremity. Number of aperture (NA) : The figure corresponding to the F-number of the camera. This is associated with the resolution and larger NA means higher resolution. Resolution : Ability of an objective for identifying adjacent two lines in the image, which is expressed in terms of the minimum distance between two points on the specimen surface. Focal depth : The depth range of a specimen, in which focusing is obtained at a time. Stopping down the aperture iris diaphragm increases the focal depth and increasing the objective NA decreases it. Field number (FN) : The diameter of the image observed through an eyepiece, represented in millimeters. Actual field of view : Diameter of the field of view, expressed as the size on the specimen surface. Total power : Objective magnification x Eyepiece magnification. (Total magnification) (Object side) : The following table shows the optical characteristics of com- binations of eyepieces and objectives. The figure on the right shows the performance data engraved on the objectives. *100XO (FN 20) Magnification Mechanical tube length Color band Objective type (on the back side) Numerical aperture Cover glass thickness Plan Objective Field number

adapter may cause a malfunction. · The power cord and AC adapter cord are vulnerable when bent or twisted. Never subject them to excessive force. · Make sure that the main switch 1 is set to “ ” (OFF) before connecting the power cord. · Always use the power cord provided by Olympus. If no power cord is provided, please select the proper power cord by referring to section “PROPER SELECTION OF THE POWER CORD” at the end of this instruction manual. 1. Connect the output connector 2 of the AC adapter to the input connec- tor 3 on the rear of the microscope. Do not tilt the microscope to the rear. Doing so could damage the output connector 2. 2. Connect the power cord’s connector 4 to connector 5 firmly. Be sure to supply power from a grounded, 3-conductor power outlet using the proper power cord. If the power outlet is not grounded properly, Olympus can no longer warrant the electrical safety performance of the equipment. 3. Connect the power cord’s plug 6 to a wall power outlet 7. (Fig. 18) ² @ ³ Fig. 17 | 5 Connecting the AC Adapter and Power Cord (Figs. 17 & 18) ASSEMBLY }Each standard set can be assembled by simply attaching the AC adapter and power cord. CAUTION CAUTION CAUTION * Shapes of the power cord’s plugs and power outlets are different by country. 7 6

(Fig. 19) }This holder accommodates the CH2-DS darkfield ring. 1. Insert the darkfield ring @ in the CH2-FH filter holder 2. 2. Push the filter holder 2 containing the darkfield ring @ into the bottom of the condenser until it clicks into place. }The darkfield ring enables darkfield observation using an objective from 4X to 40X. ² @ OPTIONAL ACCESSORIES 10-1 System Diagram of Optional Accessories 10-2 Installation and Operation of Optional Accessories Eyepieces WHC15X WHC15X-H Filter Holder CH2-FH Reflection Mirror CH20-MM 45 mm filter Darkfield Ring CH2-DS Microscope Frame CX22LED Eyepiece micrometer Cord Hanger CX22LED-CH Reticle holder 20.4RH

(Fig. 20) }The reflection mirror is designed to be used in microscopic observation in a location where power supply is not available. It makes it possible to use the natural light in place of the LED illumination. Fig. 20 The microscope should be installed near a window, in a place that is not exposed to direct sunlight. The eyepieces should be oriented toward the rear so that the reflection mirror receives the bright light. Loosen the observation tube clamping knob and rotate the tube by 180°. 1. Fit the reflection mirror in the window lens of the microscope frame by aligning the mounting tab. 2. The reflection mirror 1 should be pointed toward the bright area. While observing the image through eyepieces, adjust the orientation of the reflection mirror. }A planar reflection mirror is used normally. However, if the image bright- ness is uneven or the outside view is visible in the image, use a concave reflection mirror. @ 3 Eyepieces WHC15X/WHC15X-H (Fig. 21) }The standard 10X eyepieces are clamped using screws. 1. Using a small flat-blade screwdriver, loosen the clamping screw 1 of a 10X eyepiece and remove it. 2. Insert the WHC15X or WHC15X-H into the eyepiece sleeve and tighten the clamping screw 1. CAUTION Fig. 21 @

mm) and two 20.4-RH reticle holders (available as 2-piece set). The field number becomes 19.6 when the reticle holders are used. If you are using WHC15X or WHC15X-H, please purchase the micrometer with 19mm diameter and 1mm thickness. }As the helicoid adjustment is not available for the eyepieces other than WHC15X-H, if your eye is poor in eyesight and cannot focus the micrometer, use eyeglasses to correct your vision. 1. Remove both eyepieces by loosening the clamping screw using a small flat-blade screwdriver. 2. Hold the micrometer disk @ with the surface with indications facing down and place it into a reticle holder ². Be careful not to leave dirt on the micrometer disk, as it will be noticeable during observation. 3. Screw the reticle holder ² containing the micrometer disk @ into the bottom of an eyepiece. Be sure to screw in all the way by hooking your nail into the notch 3 on the holder at the end. 4. Screw the other reticle holder, alone, into the other eyepiece in order to align the field number. 5. Attach the eyepieces again, and tighten the clamping screw. 4 Using the Eyepiece Micrometer Disk (Optional) (Fig. 22) Fig. 22 ² @ 3 Attachment }When the cord hanger (CX22LED-CH) is attached on the back of the microscope frame, the power cord and AC adapter can be wound around it for storing. 1. Align the hooks ² of the cord hanger @ with the cord hanger mounting position 3, insert the cord hanger, push it firmly against the microscope frame and slide it down to lock it. 5 Cord Hanger CX22LED-CH (Figs. 23 to 29) Fig. 23 ² @ 3 CAUTION Fig. 24 Storage 1. Insert the connector @ of the power cord firmly into the input connector ² of the AC adapter (Fig. 24). }Be sure to unplug the power cord from the outlet in advance. ² @

3. Bring the cable 5 of the AC adapter into the hole of center part b of cord hanger. Then, bring it down so that it goes around the bottom part c of cord hanger and bring it into the hole b again (Fig. 26). 4. Insert the connector 6 of the AC adapter firmly into the connector 7 of the microscope (Fig. 27). ³ | 5 7 6

hanger. If it is detached during transport, the microscope may drop and human injury may result. · Be sure to disconnect the AC adapter from the microscope when storing the microscope after use. CAUTION Fig. 28 Fig. 29 5. Place the power cord ‡ on the cord hanger 4 as illustlated (Fig. 28). Removal 1. Insert the tool 9 (such as screwdriver) into the space between the pin a of cord hanger 4 and the microscope. 2. Keep the tool 9 in the space and pull it approx. 10 mm (in the direction 1 ). Then take the cord hanger 4 up (in the direction 2 ) to remove from the microscope. | ‡ | a 9

If no power cord is provided, please select the proper power cord for the equipment by referring to “ Specifications ” and “ Certified Cord ” below: CAUTION: In case you use a non-approved power cord for Olympus products, Olympus can no longer warrant the electrical safety of the equipment. Specifications Voltage Rating Current Rating Temperature Rating Length Fittings Configuration 125V AC (for 100-120V AC area) or, 250V AC (for 220-240V AC area) 6A minimum 60°C minimum 3.05 m maximum Grounding type attachment plug cap. Opposite terminates in molded-on IEC con- figuration appliance coupling. Table 1 Certified Cord A power cord should be certified by one of the agencies listed in Table 1 , or comprised of cordage marked with an agency marking per Table 1 or marked per Table 2. The fittings are to be marked with at least one of agencies listed in Table 1. In case you are unable to buy locally in your country the power cord which is approved by one of the agencies mentioned in Table 1, please use replacements approved by any other equivalent and authorized agencies in your country. Country Agency Certification Mark Country Agency Certification Mark Argentina Australia Austria Belgium Canada Denmark Finland France Germany Ireland IRAM SAA ÖVE CEBEC CSA DEMKO FEI UTE VDE NSAI Italy Japan Netherlands Norway Spain Sweden Switzerland United Kingdom U.S.A. IMQ ASTA BSI UL KEMA NEMKO AEE SEMKO SEV JET, JQA, TÜV, UL Japan / METI

APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS Approval Organization Printed or Embossed Harmoniza- tion Marking (May be located on jacket or insulation of internal wir- ing) Alternative Marking Utilizing Black-Red-Yellow Thread (Length of color section in mm) Black Red Yellow Comite Electrotechnique Belge (CEBEC) Verband Deutscher Elektrotechniker (VDE) e.V. Prüfstelle Union Technique de l´Electricite´ (UTE) Instituto Italiano del Marchio di Qualita´ (IMQ) British Approvals Service for Electric Cables (BASEC) N.V. KEMA SEMKO AB Svenska Elektriska Materielkontrollanstalter Österreichischer Verband für Elektrotechnik (ÖVE) Danmarks Elektriske Materialkontroll (DEMKO) National Standards Authority of Ireland (NSAI) Norges Elektriske Materiellkontroll (NEMKO) Asociacion Electrotecnica Y Electronica Espanola (AEE) Hellenic Organization for Standardization (ELOT) Instituto Portages da Qualidade (IPQ) Schweizerischer Elektro Technischer Verein (SEV) Elektriska Inspektoratet CEBEC USE IEMMEQU BASEC KEMA-KEUR SEMKO <ÖVE> NEMKO ELOT np SEV SETI 10 30 10 30 10 10 30 10 30 10 30 50 10 10 30 10 30 30 10 10 50 30 10 50 30 10 30 30 30 50 10 10 70 30 10 70 30 30 70 10 10 90 10 30 90 10 30 90 Underwriters Laboratories Inc. (UL) SV, SVT, SJ or SJT, 3 X 18AWG Canadian Standards Association (CSA) SV, SVT, SJ or SJT, 3 X 18AWG

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